FMOC-Lys(Cy5)-OH

FMOC-Lys(Cy5)-OH is a building block for in-sequence Lys labeling by Cy5. Cy5 is one of the most common fluorescent dyes for preparing FRET peptides (often paired with Cy3 or TQ5).

Protocol

SDS

Catalog	Size	Price	Quantity
5055	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(Cy5)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	89.187 µL	445.935 µL	891.87 µL	4.459 mL	8.919 mL
5 mM	17.837 µL	89.187 µL	178.374 µL	891.87 µL	1.784 mL
10 mM	8.919 µL	44.593 µL	89.187 µL	445.935 µL	891.87 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1121.24 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7

Authors:

Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi

Journal:

Molecules (2014): 2481--2496

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

Authors:

Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L

Journal:

Nature protocols (2013): 1787--1799

References

View all 16 references: Citation Explorer

Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach

Authors:

Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.

Journal:

Biochemistry (2009): 5753

A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization

Authors:

Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.

Journal:

Nucleic Acids Res (2009): e116

Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds

Authors:

Nettels D, Hoffmann A, Schuler B.

Journal:

J Phys Chem B (2008): 6137

Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection

Authors:

Bruno JG, Carrillo MP, Phillips T.

Journal:

J Biomol Tech (2008): 109

Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil

Authors:

Oquare BY, Taylor JS.

Journal:

Bioconjug Chem (2008): 2196

Physical properties

Molecular weight	1121.24
Solvent	DMF

Spectral properties

Correction factor (260 nm)	0.02
Correction factor (280 nm)	0.03
Correction factor (482 nm)	0.009
Correction factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27 ¹ , 0.4 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	312961-84-5

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Peptide Labeling Reagents

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Page updated on October 31, 2025