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FMOC-Lys(Cy5)-OH

FMOC-Lys(Cy5)-OH is a building block for in-sequence Lys labeling by Cy5. Cy5 is one of the most common fluorescent dyes for preparing FRET peptides (often paired with Cy3 or TQ5).

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(Cy5)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM89.187 µL445.935 µL891.87 µL4.459 mL8.919 mL
5 mM17.837 µL89.187 µL178.374 µL891.87 µL1.784 mL
10 mM8.919 µL44.593 µL89.187 µL445.935 µL891.87 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Product family

NameExtinction coefficient (cm -1 M -1)Correction Factor (280 nm)Correction Factor (260 nm)
FMOC-Lys(DABCYL)-OH *CAS 146998-27-8*-0.516-
FMOC-Lys(TF3)-OH7500010.179-
FMOC-Lys(Cy3)-OH15000010.0730.07
FMOC-Lys(TQ2)-OH210000.120.100
FMOC-Lys(TQ3)-OH220000.0910.085

Citations

View all 2 citations: Citation Explorer
Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799

References

View all 16 references: Citation Explorer
Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
Page updated on October 8, 2024

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Catalog Number5055
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Physical properties

Molecular weight

1121.24

Solvent

DMF

Spectral properties

Correction Factor (260 nm)

0.02

Correction Factor (280 nm)

0.03

Correction Factor (482 nm)

0.009

Correction Factor (565 nm)

0.09

Extinction coefficient (cm -1 M -1)

2500001

Excitation (nm)

651

Emission (nm)

670

Quantum yield

0.271, 0.42

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

312961-84-5
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