FMOC-Lys(DABCYL)-OH *CAS 146998-27-8*

FMOC-Lys(DABCYL)-OH

CAS 146998-27-8

FMOC-Lys(DABCYL)-OH is a building block for in-sequence Lys labeling by DABCYL. DABCYL is one of the most common acceptor dyes for preparing FRET peptides (often paired with EDANS or TF1).

Protocol

COA

Catalog	Size	Price	Quantity
5040	100 mg	Price
5041	1 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(DABCYL)-OH *CAS 146998-27-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	161.366 µL	806.829 µL	1.614 mL	8.068 mL	16.137 mL
5 mM	32.273 µL	161.366 µL	322.732 µL	1.614 mL	3.227 mL
10 mM	16.137 µL	80.683 µL	161.366 µL	806.829 µL	1.614 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	619.71 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Adjusting the Structure of a Peptide Nucleic Acid (PNA) Molecular Beacon and Promoting Its DNA Detection by a Hybrid with Quencher-Modified DNA

Authors:

Shigeto, Hajime and Kishi, Takamasa and Ishii, Koki and Ohtsuki, Takashi and Yamamura, Shohei and Kitamatsu, Mizuki

Journal:

Processes (2022): 722

Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7

Authors:

Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi

Journal:

Molecules (2014): 2481--2496

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

Authors:

Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L

Journal:

Nature protocols (2013): 1787--1799

References

View all 16 references: Citation Explorer

Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach

Authors:

Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.

Journal:

Biochemistry (2009): 5753

A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization

Authors:

Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.

Journal:

Nucleic Acids Res (2009): e116

Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds

Authors:

Nettels D, Hoffmann A, Schuler B.

Journal:

J Phys Chem B (2008): 6137

Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection

Authors:

Bruno JG, Carrillo MP, Phillips T.

Journal:

J Biomol Tech (2008): 109

Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil

Authors:

Oquare BY, Taylor JS.

Journal:

Bioconjug Chem (2008): 2196

Physical properties

Molecular weight	619.71
Solvent	DMF

Spectral properties

Absorbance (nm)	454
Correction factor (280 nm)	0.516

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	146998-27-8

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