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FMOC-Lys(DABCYL)-OH *CAS 146998-27-8*

FMOC-Lys(DABCYL)-OH is a building block for in-sequence Lys labeling by DABCYL. DABCYL is one of the most common acceptor dyes for preparing FRET peptides (often paired with EDANS or TF1).

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(DABCYL)-OH *CAS 146998-27-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM161.366 µL806.829 µL1.614 mL8.068 mL16.137 mL
5 mM32.273 µL161.366 µL322.732 µL1.614 mL3.227 mL
10 mM16.137 µL80.683 µL161.366 µL806.829 µL1.614 mL

Molarity calculator

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Spectrum

Product family

NameExtinction coefficient (cm -1 M -1)Correction Factor (280 nm)Correction Factor (260 nm)
FMOC-Lys(TF3)-OH7500010.179-
FMOC-Lys(Cy3)-OH15000010.0730.07
FMOC-Lys(Cy5)-OH25000010.030.02
FMOC-Asp(DABCYL)-OH-0.516-
FMOC-Glu(DABCYL)-OH-0.516-
FMOC-Lys(TQ2)-OH210000.120.100
FMOC-Lys(TQ3)-OH220000.0910.085

Citations

View all 3 citations: Citation Explorer
Adjusting the Structure of a Peptide Nucleic Acid (PNA) Molecular Beacon and Promoting Its DNA Detection by a Hybrid with Quencher-Modified DNA
Authors: Shigeto, Hajime and Kishi, Takamasa and Ishii, Koki and Ohtsuki, Takashi and Yamamura, Shohei and Kitamatsu, Mizuki
Journal: Processes (2022): 722
Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799

References

View all 16 references: Citation Explorer
Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
Page updated on November 1, 2024

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Physical properties

Molecular weight

619.71

Solvent

DMF

Spectral properties

Absorbance (nm)

454

Correction Factor (280 nm)

0.516

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

146998-27-8
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