FMOC-Lys(TF2-Boc)-OH

FMOC-Lys(TF2-Boc)-OH is a building block for in-sequence Lys labeling by TF2, an excellent and more affordable replacement for the least affordable Alexa Fluor 488. TF2 is one of the most common donor dyes for preparing FRET peptides (often paired with TAMRA or TQ2) since TF2 and Alexa Fluor 488 share the same R110 fluorophore.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5050	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(TF2-Boc)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	108.109 µL	540.546 µL	1.081 mL	5.405 mL	10.811 mL
5 mM	21.622 µL	108.109 µL	216.219 µL	1.081 mL	2.162 mL
10 mM	10.811 µL	54.055 µL	108.109 µL	540.546 µL	1.081 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	924.99 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7

Authors:

Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi

Journal:

Molecules (2014): 2481--2496

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

Authors:

Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L

Journal:

Nature protocols (2013): 1787--1799

References

View all 16 references: Citation Explorer

Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach

Authors:

Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.

Journal:

Biochemistry (2009): 5753

A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization

Authors:

Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.

Journal:

Nucleic Acids Res (2009): e116

Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds

Authors:

Nettels D, Hoffmann A, Schuler B.

Journal:

J Phys Chem B (2008): 6137

Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection

Authors:

Bruno JG, Carrillo MP, Phillips T.

Journal:

J Biomol Tech (2008): 109

Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil

Authors:

Oquare BY, Taylor JS.

Journal:

Bioconjug Chem (2008): 2196

Physical properties

Molecular weight	924.99
Solvent	DMF

Spectral properties

Correction factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	75000
Excitation (nm)	503
Emission (nm)	525
Quantum yield	0.9 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on April 6, 2022