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FMOC-Lys(TF3)-OH
FMOC-Lys(TF3)-OH is a building block for in-sequence Lys labeling by TF3. TF3 has the spectral properties identical to Cy3. Compared to Cy3, TF3 is more stable to acidic and basic deprotection conditions. Our TF3 is also more fluorescent than Cy3 on peptides and oligonucleotides.
Calculators
Common stock solution preparation
Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Lys(TF3)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 123.622 µL | 618.108 µL | 1.236 mL | 6.181 mL | 12.362 mL |
| 5 mM | 24.724 µL | 123.622 µL | 247.243 µL | 1.236 mL | 2.472 mL |
| 10 mM | 12.362 µL | 61.811 µL | 123.622 µL | 618.108 µL | 1.236 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) | Correction Factor (260 nm) |
| FMOC-Asp(TF3)-OH | 554 | 578 | 750001 | 0.179 | - |
| FMOC-Glu(TF3)-OH | 554 | 578 | 750001 | 0.179 | - |
| FMOC-Lys(DABCYL)-OH *CAS 146998-27-8* | - | - | - | 0.516 | - |
| FMOC-Lys(Cy3)-OH | 555 | 569 | 1500001 | 0.073 | 0.07 |
| FMOC-Lys(Cy5)-OH | 651 | 670 | 2500001 | 0.03 | 0.02 |
| FMOC-Lys(TQ2)-OH | - | - | 21000 | 0.12 | 0.100 |
| FMOC-Lys(TQ3)-OH | - | - | 22000 | 0.091 | 0.085 |
Citations
View all 2 citations: Citation Explorer
Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496
Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799
References
View all 16 references: Citation Explorer
Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116
Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109
Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196
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