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Forte Blue™ DHPE

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Physical properties
Molecular weight944.13
SolventDMSO
Spectral properties
Excitation (nm)356
Emission (nm)456
Storage, safety and handling
H-phraseH301, H311, H331
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR23, R24, R25
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
944.13
Excitation (nm)
356
Emission (nm)
456
Forte Blue™ DHPE is the same molecule to Marina Blue® 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (Marina Blue® DHPE, Marina Blue® is the trademark of ThermoFisher). It is a phospholipid that is labeled on the head group with the bright, blue-fluorescent Forte Blue™ dye. Forte Blue™ DHPE is optimally excited by the intense 365 nm spectral line of the mercury-arc lamp/UV lasers, and exhibits bright blue fluorescence emission near 460 nm. Significantly, the low pKa value of this newly developed 7-hydroxycoumarin derivative is 2"3 log units lower than that of the parent 7-hydroxycoumarin analogs. Forte Blue™ DHPE is expected to be strongly fluorescent in membranes, even at neutral pH.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Forte Blue™ DHPE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM105.918 µL529.588 µL1.059 mL5.296 mL10.592 mL
5 mM21.184 µL105.918 µL211.835 µL1.059 mL2.118 mL
10 mM10.592 µL52.959 µL105.918 µL529.588 µL1.059 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)356
Emission (nm)456

Images


Citations


View all 1 citations: Citation Explorer
Development of PEGylated aspartic acid-modified liposome as a bone-targeting carrier for the delivery of paclitaxel and treatment of bone metastasis
Authors: Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira
Journal: Biomaterials (2017)

References


View all 27 references: Citation Explorer
Permeabilization assay for antimicrobial peptides based on pore-spanning lipid membranes on nanoporous alumina
Authors: Neubacher H, Mey I, Carnarius C, Lazzara TD, Steinem C.
Journal: Langmuir (2014): 4767
Membrane order parameters for interdigitated lipid bilayers measured via polarized total-internal-reflection fluorescence microscopy
Authors: Ngo AT, Jakubek ZJ, Lu Z, Joos B, Morris CE, Johnston LJ.
Journal: Biochim Biophys Acta (2014): 2861
Mesoporous gold and palladium nanoleaves from liquid-liquid interface: enhanced catalytic activity of the palladium analogue toward hydrazine-assisted room-temperature 4-nitrophenol reduction
Authors: Dutta S, Sarkar S, Ray C, Roy A, Sahoo R, Pal T.
Journal: ACS Appl Mater Interfaces (2014): 9134
Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species
Authors: Hu SK, Chen YM, Chao L.
Journal: Biomicrofluidics (2014): 52005
Lipid reassembly in asymmetric Langmuir-Blodgett/Langmuir-Schaeffer bilayers
Authors: Yuan J, Hao C, Chen M, Berini P, Zou S.
Journal: Langmuir (2013): 221
Coupling supported lipid bilayer electrophoresis with matrix-assisted laser desorption/ionization-mass spectrometry imaging
Authors: Pace HP, Sherrod SD, Monson CF, Russell DH, Cremer PS.
Journal: Anal Chem (2013): 6047
Calcein release behavior from liposomal bilayer; influence of physicochemical/mechanical/structural properties of lipids
Authors: Maherani B, Arab-Tehrany E, Kheirolomoom A, Geny D, Linder M.
Journal: Biochimie (2013): 2018
In vivo digestion of bovine milk fat globules: effect of processing and interfacial structural changes. I. Gastric digestion
Authors: Gallier S, Cui J, Olson TD, Rutherfurd SM, Ye A, Moughan PJ, Singh H.
Journal: Food Chem (2013): 3273
Rapid detection of several foodborne pathogens by F0F1-ATPase molecular motor biosensor
Authors: Zhang J, Li Z, Zhang H, Wang J, Liu Y, Chen G.
Journal: J Microbiol Methods (2013): 37
Formation and diffusivity characterization of supported lipid bilayers with complex lipid compositions
Authors: Simonsson L, Hook F.
Journal: Langmuir (2012): 10528