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Fura-10™, AM
Among ratiometric calcium ion indicators, Fura-2 and Indo-1 are the two most popular ones. However, there are still a few challenges for using these two calcium ion indicators, in particular, for live cells. UV-excitation of Fura 2 caused fast photobleaching. Fura-8™ was introduced a few years ago to shift the excitation closer to visible light. Although Fura-8 demonstrated significant improvement in the ratio of signal/background, it is not well retained in live cells just like Fura-2. Fura-10 have recently been introduced to address this cellular retention issue. Fura 10 demonstrated dramatic improvement in the ratio of signal/background in the absence of probenecid.
<strong>ATP-stimulated calcium response of endogenous P2Y receptor in CHO-K1 cells measured with </strong><strong>Fura-2 AM, Fura-8&trade; AM and Fura-10&trade; AM in the absence of Probenecid. </strong>CHO-K1cells were seeded overnight in 50,000 cells per 100 &micro;L per well in a 96-well black wall/clear bottom costar plate. 100 &micro;L of 5&nbsp;uM&nbsp;Fura-2 AM or Fura-8&trade; AM or Fura-10&trade; AM without probenecid was added into the cells, and the cells were incubated at 37 <sup>o</sup>C for 45 minutes and RT for 30 minutes. &nbsp;ATP (50&micro;L/well) was added by FlexStation (Molecular Devices) to achieve the final indicated concentrations.
<strong>ATP-stimulated calcium response of endogenous P2Y receptor in CHO-K1 cells measured with </strong><strong>Fura-2 AM, Fura-8&trade; AM and Fura-10&trade; AM in the absence of Probenecid. </strong>CHO-K1cells were seeded overnight in 50,000 cells per 100 &micro;L per well in a 96-well black wall/clear bottom costar plate. 100 &micro;L of 5&nbsp;uM&nbsp;Fura-2 AM or Fura-8&trade; AM or Fura-10&trade; AM without probenecid was added into the cells, and the cells were incubated at 37 <sup>o</sup>C for 45 minutes and RT for 30 minutes. &nbsp;ATP (50&micro;L/well) was added by FlexStation (Molecular Devices) to achieve the final indicated concentrations.
protocol
Protocol
COA
CatalogSize
Price
Quantity
211145x50 ug
Price
211151 mg
Price
 
Physical properties
Dissociation constant (Kd, nM)260
Molecular weight1194.14
SolventDMSO
Spectral properties
Excitation (nm)354
Emission (nm)524
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Fluorescence microplate reader
Excitation354 nm and 415 nm
Emission524 nm
Cutoff475 nm
Recommended plateBlack wall/Clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
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Page updated on October 8, 2025