Fura-10™, AM
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Catalog Number | |
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Dissociation constant (Kd, nM) | 260 |
Molecular weight | 1194.14 |
Solvent | DMSO |
Excitation (nm) | 354 |
Emission (nm) | 524 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Fura-10™, potassium salt |
Overview | SDSProtocol |
Molecular weight 1194.14 | Dissociation constant (Kd, nM) 260 | Excitation (nm) 354 | Emission (nm) 524 |
Platform
Fluorescence microplate reader
Excitation | 354 nm and 415 nm |
Emission | 524 nm |
Cutoff | 475 nm |
Recommended plate | Black wall/Clear bottom |
Instrument specification(s) | Bottom read mode/Programmable liquid handling |
Example protocol
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Prepare a 2 to 5 mM Fura-10™ AM stock solution in high-quality, anhydrous DMSO.
PREPARATION OF WORKING SOLUTION
On the day of the experiment, either dissolve Fura-10™ AM in DMSO or thaw an aliquot of the indicator stock solution to room temperature.
Prepare a 2 to 20 µM Fura-10™ AM working solution in a buffer of your choice (e.g., Hanks and Hepes buffer) with 0.04% Pluronic® F-127. For most cell lines, Fura-10™ AM at a final concentration of 4-5 μM is recommended. The exact concentration of indicators required for cell loading must be determined empirically.
Note: The nonionic detergent Pluronic® F-127 is sometimes used to increase the aqueous solubility of Fura-10™ AM. A variety of Pluronic® F-127 solutions can be purchased from AAT Bioquest.
Note: If your cells contain organic anion-transporters, probenecid (1-2 mM) may be added to the dye working solution (final in well concentration will be 0.5-1 mM) to reduce leakage of the de-esterified indicators. A variety of ReadiUse™ Probenecid products, including water-soluble, sodium salt, and stabilized solutions, can be purchased from AAT Bioquest.
SAMPLE EXPERIMENTAL PROTOCOL
Following is our recommended protocol for loading AM esters into live cells. This protocol only provides a guideline and should be modified according to your specific needs.
- Prepare cells in growth medium overnight.
On the next day, add 1X Fura-10™ AM working solution to your cell plate.
Note: If your compound(s) interfere with the serum, replace the growth medium with fresh HHBS buffer before dye-loading.
Incubate the dye-loaded plate in a cell incubator at 37 °C for 30 to 60 minutes.
Note: Incubating the dye for longer than 1 hour can improve signal intensities in certain cell lines.
- Replace the dye working solution with HHBS or buffer of your choice (containing an anion transporter inhibitor, such as 1 mM probenecid, if applicable) to remove any excess probes.
- Add the stimulant as desired and simultaneously monitor fluorescence intensity using a fluorescence plate reader containing a programmable liquid handling system such as a FlexStation, at Ex/Em1 = 354/524 nm cutoff 475 nm and Ex/Em2 = 415/524 nm cutoff 475 nm.
Calculators
Common stock solution preparation
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 83.742 µL | 418.711 µL | 837.423 µL | 4.187 mL | 8.374 mL |
5 mM | 16.748 µL | 83.742 µL | 167.485 µL | 837.423 µL | 1.675 mL |
10 mM | 8.374 µL | 41.871 µL | 83.742 µL | 418.711 µL | 837.423 µL |
Molarity calculator
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) |
Fura-8FF™, AM | 354 | 524 |
Fura-2, AM *CAS 108964-32-5* | 336 | 505 |
Fura-2, AM *UltraPure Grade* *CAS 108964-32-5* | 336 | 505 |
Fura-FF, AM [Fura-2FF, AM] *CAS 348079-12-9* | 336 | 505 |
Fura Red, AM *CAS 149732-62-7* | 435 | 639 |
Fura-8™, AM | 354 | 524 |
Images
Citations
Authors: Sullivan, Matthew Ryan
Journal: (2023)
References
Authors: Jeong, Dahye and Irfan, Muhammad and Lee, Dong-Ha and Hong, Seung-Bok and Oh, Jae-Wook and Rhee, Man Hee
Journal: BMC complementary medicine and therapies (2020): 98
Authors: Hu, Yingru and Xia, Weijie and Li, Yingsha and Wang, Qianran and Lin, Shaoyang and Wang, Bin and Zhou, Cui and Cui, Yuanting and Jiang, Yanli and Pu, Xiaona and Wei, Xiao and Wu, Hao and Zhang, Hengshu and Zhu, Zhiming and Liu, Daoyan and Li, Zhiyong
Journal: Hypertension research : official journal of the Japanese Society of Hypertension (2020)
Authors: Schach, Christian and Wester, Michael and Leibl, Florian and Redel, Andreas and Gruber, Michael and Maier, Lars S and Endemann, Dierk and Wagner, Stefan
Journal: Clinical and experimental pharmacology & physiology (2020)
Authors: Zhang, Yi and Du, Ning-Yu and Chen, Chen and Wang, Tong and Wang, Li-Juan and Shi, Xiao-Lu and Li, Shu-Ming and Guo, Chang-Qing
Journal: Evidence-based complementary and alternative medicine : eCAM (2020): 5129562
Authors: Sophocleous, Reece A and Berg, Tracey and Finol-Urdaneta, Rocio K and Sluyter, Vanessa and Keshiya, Shikara and Bell, Lachlan and Curtis, Stephen J and Curtis, Belinda L and Seavers, Aine and Bartlett, Rachael and Dowton, Mark and Stokes, Leanne and Ooi, Lezanne and Sluyter, Ronald
Journal: British journal of pharmacology (2020)
Authors: Berra-Romani, Roberto and Faris, Pawan and Pellavio, Giorgia and Orgiu, Matteo and Negri, Sharon and Forcaia, Greta and Var-Gaz-Guadarrama, Verónica and Garcia-Carrasco, Mario and Botta, Laura and Sancini, Giulio and Laforenza, Umberto and Moccia, Francesco
Journal: Journal of cellular physiology (2020): 1515-1530
Authors: Amini, K and Zhaleh, H and Tahvilian, R and Farnia, V
Journal: Bratislavske lekarske listy (2019): 256-262
Authors: Wiebe, Shane A and Plain, Allein and Pan, Wanling and O'Neill, Debbie and Braam, Branko and Alexander, R Todd
Journal: American journal of physiology. Renal physiology (2019): F240-F253
Authors: Keyvanloo Shahrestanaki, Mohammad and Aghaei, Mahmoud
Journal: Research in pharmaceutical sciences (2019): 107-114
Authors: Huang, Xing and Hu, Yanxin and Zhao, Li and Gu, Boya and Zhu, Rongxin and Li, Yan and Yang, Yun and Han, Tianyu and Yu, Jiabei and Mu, Lianwei and Han, Peng and Li, Cui and Zhang, Weijia and Hu, Yang
Journal: Saudi journal of biological sciences (2019): 1194-1206
Application notes
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
Novel Red Fluorescent Calcium Probes for Functional Analysis of GPCRs and Calcium Channel Targets
FAQ
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
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