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Fura-2, AM
CAS 108964-32-5
Fura-2 AM is a UV-excitable ratiometric calcium indicator that shifts excitation wavelength upon calcium binding, enabling quantitative measurement of intracellular calcium concentrations independent of dye loading and cell thickness.
  • Ratiometric Calcium Measurement: Excitation shift from 380 nm (free) to 340 nm (Ca²⁺-bound)
  • Quantitative [Ca²⁺] Determination: Kd = 145 nM, independent of dye concentration or cell thickness
  • UV-Excitable Design: Emission at 510 nm allows multicolor experiments with visible dyes
  • Calibration-Friendly: Rmax/Rmin determination enables absolute calcium quantification
Fluorescence excitation spectra of Fura-2 in solutions conctaining 0 to 39uM free Ca2+.
Fluorescence excitation spectra of Fura-2 in solutions conctaining 0 to 39uM free Ca2+.
CatalogSize
Price
Quantity
210201 mg
Price
2102250 mg
Price
 
Physical properties

Dissociation constant (Kd, nM)145
Molecular weight1001.86
SolventDMSO
Spectral properties

Excitation (nm)336
Emission (nm)505
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
CAS108964-32-5
Instrument settings

Fluorescence microscope
ExcitationFura 2 filter set
EmissionFura 2 filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation340, 380
Emission510
Cutoff475
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
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Page updated on October 12, 2025