Name: Fura-2, pentasodium salt
Brand: AAT Bioquest
SKU: 21026
Price: 118 USD
Availability: InStock

Fura-2, pentasodium salt

Among the ratiometric calcium indicators, Fura-2 and Indo-1 are most commonly used. Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. Fura-2 sodium salt is a cell-impermeable fluorescent probe for detection Ca2+ in a ratiometric mode.

Datasheet

SDS

Catalog	Size	Price	Quantity
21026	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fura-2, pentasodium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	133.076 µL	665.38 µL	1.331 mL	6.654 mL	13.308 mL
5 mM	26.615 µL	133.076 µL	266.152 µL	1.331 mL	2.662 mL
10 mM	13.308 µL	66.538 µL	133.076 µL	665.38 µL	1.331 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	751.45 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

An essential role of NAD (P) H oxidase 2 in UVA-induced calcium oscillations in mast cells

Authors:

Li, Zhi Ying and Jiang, Wen Yi and Cui, Zong Jie

Journal:

Photochemical & Photobiological Sciences (2015): 414--428

Lasting inhibition of receptor-mediated calcium oscillations in pancreatic acini by neutrophil respiratory burst--A novel mechanism for secretory blockade in acute pancreatitis?

Authors:

Liang, Hui Yuan and Song, Zhi Min and Cui, Zong Jie

Journal:

Biochemical and biophysical research communications (2013): 361--367

References

View all 119 references: Citation Explorer

Load of calcium probe Fura -2/AM in Escherichia coli cells

Authors:

Shao M, Wang HM, Liu ZH, Shen P, Cai RX.

Journal:

Wei Sheng Wu Xue Bao (2005): 805

An Excel-based model of Ca2+ diffusion and fura 2 measurements in a spherical cell

Authors:

McHugh JM, Kenyon JL.

Journal:

Am J Physiol Cell Physiol (2004): C342

Problems caused by high concentration of ATP on activation of the P2X7 receptor in bone marrow cells loaded with the Ca2+ fluorophore fura-2

Authors:

Paredes-Gamero EJ, Franca JP, Moraes AA, Aguilar MO, Oshiro ME, Ferreira AT.

Journal:

J Fluoresc (2004): 711

Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2

Authors:

McConnell G, Riis E.

Journal:

Phys Med Biol (2004): 4757

Abnormal spectra alteration observed in Triton calibration method for measuring [Ca2+]i with fluorescence indicator, fura-2

Authors:

Xu T, Yang W, Huo XL, Song T.

Journal:

J Biochem Biophys Methods (2004): 219

Physical properties

Dissociation constant (K_d, nM)	145
Molecular weight	751.45
Solvent	Water

Spectral properties

Excitation (nm)	336
Emission (nm)	505

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on July 18, 2026