Name: Fura-8™, pentapotassium salt
Brand: AAT Bioquest
SKU: 21057
Price: 115 USD
Availability: InStock

Fura-8™, pentapotassium salt

Although Fura-2 has become the preferred excitation-ratioable calcium indicator of choice, it has certain limitations (e.g., lower sensitivity than single wavelength calcium indicators such as Fluo-8® and Cal-520®). To address these concerns, AAT Bioquest has devoted considerable efforts to the development of Fura™ 8, a high-affinity ratiometric calcium indicator with improved sensitivity and higher signal-to-noise ratios. The fluorescence emission of Fura™ 8 is red-shifted to a longer visible wavelength, facilitating the detection of Fura™ 8 by common filter sets. Fura™ 8, pentapotassium salt is membrane-impermeant and is excited at 355 nm and 415 nm and emits at 530 nm.

Fluorescence excitation spectra of Fura-8™ in the presence of 0 to 39 µM free Ca2+.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21057	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fura-8™, pentapotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	122.548 µL	612.738 µL	1.225 mL	6.127 mL	12.255 mL
5 mM	24.51 µL	122.548 µL	245.095 µL	1.225 mL	2.451 mL
10 mM	12.255 µL	61.274 µL	122.548 µL	612.738 µL	1.225 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	816.01 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

Nifedipine stimulates proliferation and migration of different breast cancer cells by distinct pathways

Authors:

Zhao, Tao and Guo, Dongqing and Gu, Yuchun and Ling, Yang

Journal:

Molecular Medicine Reports (2017): 2259--2263

Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to the development of pulmonary arterial hypertension

Authors:

Guo, Dongqing and Gu, Junzhong and Jiang, Hui and Ahmed, Asif and Zhang, Zhiren and Gu, Yuchun

Journal:

Journal of molecular and cellular cardiology (2016): 179--187

Nifedipine promotes the proliferation and migration of breast cancer cells

Authors:

Guo, Dong-Qing and Zhang, Hao and Tan, Sheng-Jiang and Gu, Yu-Chun

Journal:

PloS one (2014): e113649

Bioanalytics/Illumination: Just-right light-Inherently adaptive for application-specific needs

Authors:

JOHNSON, IAIN

References

View all 84 references: Citation Explorer

Measurement of [Ca2+] in cell suspensions using indo-1

Authors:

Nelemans A., undefined

Journal:

Methods Mol Biol (2006): 47

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux

Authors:

Bailey S, Macardle PJ.

Journal:

J Immunol Methods (2006): 220

Ratiometric intracellular calcium imaging in the isolated beating rat heart using indo-1 fluorescence

Authors:

Eerbeek O, Mik EG, Zuurbier CJ, van 't Loo M, Donkersloot C, Ince C.

Journal:

J Appl Physiol (2004): 2042

Negative inotropic effects of angiotensin II, endothelin-1 and phenylephrine in indo-1 loaded adult mouse ventricular myocytes

Authors:

Sakurai K, Norota I, Tanaka H, Kubota I, Tomoike H, Endo M.

Journal:

Life Sci (2002): 1173

Usefulness of the analytic method of intracellular calcium and the problems--aequorin and indo-1 signal

Authors:

Endoh M., undefined

Journal:

Nippon Yakurigaku Zasshi (2000): 361

Physical properties

Dissociation constant (K_d, nM)	260
Molecular weight	816.01
Solvent	Water

Spectral properties

Excitation (nm)	354
Emission (nm)	524

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025