Fura-8FF™, AM

The cell-permeant Fura-8FF AM is an analog of Fura-8 AM with much lower calcium binding affinity, Kd ~10 µM. Fura-8FF has its emission shifted into longer visible wavelength that is compatible with the common filter sets. Fura-8FF™ AM is more sensitive to calcium than Fura-2FF AM with higher signal/background ratio than that of Fura-2FF AM. e., calculating the excitation intensity ratios at 354 nm and 415 nm by monitoring emission intensity at 530 nm.

Fluorescence excitation spectra of Fura-8™ in the presence of 0 to 39 µM free Ca2+.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20620	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fura-8FF™, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	102.684 µL	513.421 µL	1.027 mL	5.134 mL	10.268 mL
5 mM	20.537 µL	102.684 µL	205.368 µL	1.027 mL	2.054 mL
10 mM	10.268 µL	51.342 µL	102.684 µL	513.421 µL	1.027 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	973.86 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

An essential role of NAD (P) H oxidase 2 in UVA-induced calcium oscillations in mast cells

Authors:

Li, Zhi Ying and Jiang, Wen Yi and Cui, Zong Jie

Journal:

Photochemical & Photobiological Sciences (2015): 414--428

Lasting inhibition of receptor-mediated calcium oscillations in pancreatic acini by neutrophil respiratory burst--A novel mechanism for secretory blockade in acute pancreatitis?

Authors:

Liang, Hui Yuan and Song, Zhi Min and Cui, Zong Jie

Journal:

Biochemical and biophysical research communications (2013): 361--367

References

View all 119 references: Citation Explorer

Load of calcium probe Fura -2/AM in Escherichia coli cells

Authors:

Shao M, Wang HM, Liu ZH, Shen P, Cai RX.

Journal:

Wei Sheng Wu Xue Bao (2005): 805

An Excel-based model of Ca2+ diffusion and fura 2 measurements in a spherical cell

Authors:

McHugh JM, Kenyon JL.

Journal:

Am J Physiol Cell Physiol (2004): C342

Problems caused by high concentration of ATP on activation of the P2X7 receptor in bone marrow cells loaded with the Ca2+ fluorophore fura-2

Authors:

Paredes-Gamero EJ, Franca JP, Moraes AA, Aguilar MO, Oshiro ME, Ferreira AT.

Journal:

J Fluoresc (2004): 711

Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2

Authors:

McConnell G, Riis E.

Journal:

Phys Med Biol (2004): 4757

Abnormal spectra alteration observed in Triton calibration method for measuring [Ca2+]i with fluorescence indicator, fura-2

Authors:

Xu T, Yang W, Huo XL, Song T.

Journal:

J Biochem Biophys Methods (2004): 219

Physical properties

Dissociation constant (K_d, nM)	6000
Molecular weight	973.86
Solvent	DMSO

Spectral properties

Excitation (nm)	354
Emission (nm)	524

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Excitation	Fura 2 filter set
Emission	Fura 2 filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	355, 415
Emission	530
Cutoff	475
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

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Page updated on October 12, 2025