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Gelite™ Green Nucleic Acid Gel Staining Kit

160 ng of 1 kb Plus DNA Ladder (ThermoFisher 10787018) in 0.9% agarose/TBE electrophoresis gel were stained with Gelite™ Green (A) and SYBR® Green (B), and imaged with 254-nm UV transilluminator using UVP Bioimaging System.
160 ng of 1 kb Plus DNA Ladder (ThermoFisher 10787018) in 0.9% agarose/TBE electrophoresis gel were stained with Gelite™ Green (A) and SYBR® Green (B), and imaged with 254-nm UV transilluminator using UVP Bioimaging System.
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Catalog Number17589
Unit Size
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Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
UNSPSC41116134

OverviewpdfSDSpdfProtocol


Gelite™ Green is a sensitive fluorescent nucleic acid gel stain for detecting nucleic acids in agarose and polyacrylamide gels. Gelite™ Green stain exhibits exceptional affinity for DNA and a large fluorescence enhancement upon binding to DNA, at least an order of magnitude greater than that of ethidium bromide when detected by photography. With a standard 300 nm UV transilluminator and photographic detection, as little as 60 pg dsDNA per band can be detected with Gelite™ Green stain. Gelite™ Green nucleic acid gel stain is nearly two orders of magnitude more sensitive than ethidium bromide for staining oligonucleotides in gels. Our Gelite™ Green Nucleic Acid Gel Staining Gel Kit includes our Gelite™ Green nucleic acid stain with an optimized and robust protocol. It provides a convenient solution for staining nucleic acid samples in gels.

Platform


Transilluminator

Excitation254 nm or 300 nm
EmissionLong path green filter (ex. SYBR or GelStar)

Components


Component A: Gelite™ Green Stain1 vial (20 µL)
Component B: 5X Gel Loading Buffer3 x 1 mL

Example protocol


PREPARATION OF WORKING SOLUTION

Add 1 μL of Gelite™ Green Stain (Component A) into 200 μL of 5X Gel Loading Buffer (Component B) to make Gelite™ Green working solution. Protect Gelite™ Green working solution from light by covering it with foil or placing it in the dark.

SAMPLE EXPERIMENTAL PROTOCOL

  1. Prepare DNA samples as you desired.
  2. Add 4 µL of Gelite™ Green working solution into 16 µL of DNA samples and mix well. Incubate at room temperature for 5 - 15 minutes prior to electrophoresis.
  3. Run gels based on your standard protocol.
  4. Image the gel with a 300 nm ultraviolet or 254 nm transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter. 

References


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Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
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Journal: J Acupunct Meridian Stud (2008): 29
Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
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Journal: Anal Biochem (2007): 87
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Journal: Biomacromolecules (2005): 2703
Implementation of accurate and fast DNA cytometry by confocal microscopy in 3D
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Journal: Cell Oncol (2005): 225
TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual-colour FISH on paraffin sections
Authors: Bink K, Walch A, Feuchtinger A, Eisenmann H, Hutzler P, Hofler H, Werner M.
Journal: Histochem Cell Biol (2001): 293
Oxazole yellow homodimer YOYO-1-labeled DNA: a fluorescent complex that can be used to assess structural changes in DNA following formation and cellular delivery of cationic lipid DNA complexes
Authors: Wong M, Kong S, Dragowska WH, Bally MB.
Journal: Biochim Biophys Acta (2001): 61
Photophysical properties of fluorescent DNA-dyes bound to single- and double-stranded DNA in aqueous buffered solution
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Journal: Photochem Photobiol (2001): 585