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Gelite™ Green Nucleic Acid Gel Staining Kit


Gelite™ Green is a sensitive fluorescent nucleic acid gel stain for detecting nucleic acids in agarose and polyacrylamide gels. Gelite™ Green stain exhibits exceptional affinity for DNA and a large fluorescence enhancement upon binding to DNA, at least an order of magnitude greater than that of ethidium bromide when detected by photography. With a standard 300 nm UV transilluminator and photographic detection, as little as 60 pg dsDNA per band can be detected with Gelite™ Green stain. Gelite™ Green nucleic acid gel stain is nearly two orders of magnitude more sensitive than ethidium bromide for staining oligonucleotides in gels. Our Gelite™ Green Nucleic Acid Gel Staining Gel Kit includes our Gelite™ Green nucleic acid stain with an optimized and robust protocol. It provides a convenient solution for staining nucleic acid samples in gels.



Excitation254 nm or 300 nm
EmissionLong path green filter (ex. SYBR or GelStar)


Example protocol


Add 1 μL of Gelite™ Green Stain (Component A) into 200 μL of 5X Gel Loading Buffer (Component B) to make Gelite™ Green working solution. Protect Gelite™ Green working solution from light by covering it with foil or placing it in the dark.


  1. Prepare DNA samples as you desired.
  2. Add 4 µL of Gelite™ Green working solution into 16 µL of DNA samples and mix well. Incubate at room temperature for 5 - 15 minutes prior to electrophoresis.
  3. Run gels based on your standard protocol.
  4. Image the gel with a 300 nm ultraviolet or 254 nm transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter. 



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Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
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Journal: Anal Biochem (2007): 87
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Journal: Biomacromolecules (2005): 2703
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Journal: Cell Oncol (2005): 225
Green-light transilluminator for the detection without photodamage of proteins and DNA labeled with different fluorescent dyes
Authors: Alba FJ, Bermudez A, Daban JR.
Journal: Electrophoresis (2001): 399
TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual-colour FISH on paraffin sections
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Oxazole yellow homodimer YOYO-1-labeled DNA: a fluorescent complex that can be used to assess structural changes in DNA following formation and cellular delivery of cationic lipid DNA complexes
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