Gelite™ Green Nucleic Acid Gel Staining Kit
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
UNSPSC | 41116134 |
Alternative formats
Gelite™ Orange Nucleic Acid Gel Staining Kit |
Related products
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See also: Cloning, DNA Fragmentation, DNA & RNA Markers and Ladders, Gel Electrophoresis, Loop-Mediated Isothermal Amplification (LAMP), Polymerase Chain Reaction (PCR), Reverse Transcription PCR (RT-PCR), RNA Purification & Analysis, PCR Detection of Viral DNA/RNA
Gelite™ Green is a sensitive fluorescent nucleic acid gel stain for detecting nucleic acids in agarose and polyacrylamide gels. Gelite™ Green stain exhibits exceptional affinity for DNA and a large fluorescence enhancement upon binding to DNA, at least an order of magnitude greater than that of ethidium bromide when detected by photography. With a standard 300 nm UV transilluminator and photographic detection, as little as 60 pg dsDNA per band can be detected with Gelite™ Green stain. Gelite™ Green nucleic acid gel stain is nearly two orders of magnitude more sensitive than ethidium bromide for staining oligonucleotides in gels. Our Gelite™ Green Nucleic Acid Gel Staining Gel Kit includes our Gelite™ Green nucleic acid stain with an optimized and robust protocol. It provides a convenient solution for staining nucleic acid samples in gels.
Platform
Transilluminator
Excitation | 254 nm or 300 nm |
Emission | Long path green filter (ex. SYBR or GelStar) |
Components
Example protocol
PREPARATION OF WORKING SOLUTION
Add 1 μL of Gelite™ Green Stain (Component A) into 200 μL of 5X Gel Loading Buffer (Component B) to make Gelite™ Green working solution. Protect Gelite™ Green working solution from light by covering it with foil or placing it in the dark.
SAMPLE EXPERIMENTAL PROTOCOL
- Prepare DNA samples as you desired.
- Add 4 µL of Gelite™ Green working solution into 16 µL of DNA samples and mix well. Incubate at room temperature for 5 - 15 minutes prior to electrophoresis.
- Run gels based on your standard protocol.
- Image the gel with a 300 nm ultraviolet or 254 nm transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.
Images
References
View all 22 references: Citation Explorer
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Journal: Lasers Surg Med (2010): 630
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Journal: Phys Chem Chem Phys (2009): 7713
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
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Journal: J Acupunct Meridian Stud (2008): 29
Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
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Journal: Anal Biochem (2007): 87
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Journal: Anal Biochem (2007): 87
DNA length evaluation using cyanine dye and fluorescence correlation spectroscopy
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Journal: Biomacromolecules (2005): 2703
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Journal: Biomacromolecules (2005): 2703
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Journal: Histochem Cell Biol (2001): 293
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Journal: Histochem Cell Biol (2001): 293
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Journal: Biochim Biophys Acta (2001): 61
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