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Gelite™ Green Nucleic Acid Gel Staining Kit
Gelite™ Green is a sensitive fluorescent nucleic acid gel stain for detecting nucleic acids in agarose and polyacrylamide gels. Gelite™ Green stain exhibits exceptional affinity for DNA and a large fluorescence enhancement upon binding to DNA, at least an order of magnitude greater than that of ethidium bromide when detected by photography. With a standard 300 nm UV transilluminator and photographic detection, as little as 60 pg dsDNA per band can be detected with Gelite™ Green stain. Gelite™ Green nucleic acid gel stain is nearly two orders of magnitude more sensitive than ethidium bromide for staining oligonucleotides in gels. Our Gelite™ Green Nucleic Acid Gel Staining Gel Kit includes our Gelite™ Green nucleic acid stain with an optimized and robust protocol. It provides a convenient solution for staining nucleic acid samples in gels.
Example protocol
PREPARATION OF WORKING SOLUTION
Add 1 μL of Gelite™ Green Stain (Component A) into 200 μL of 5X Gel Loading Buffer (Component B) to make Gelite™ Green working solution. Protect Gelite™ Green working solution from light by covering it with foil or placing it in the dark.
SAMPLE EXPERIMENTAL PROTOCOL
- Prepare DNA samples as you desired.
- Add 4 µL of Gelite™ Green working solution into 16 µL of DNA samples and mix well. Incubate at room temperature for 5 - 15 minutes prior to electrophoresis.
- Run gels based on your standard protocol.
- Image the gel with a 300 nm ultraviolet or 254 nm transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.
References
View all 22 references: Citation Explorer
Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
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