Name: Gelite™ Orange Nucleic Acid Gel Staining Kit
Brand: AAT Bioquest
SKU: 17594
Price: 232 USD
Availability: InStock

Gelite™ Orange Nucleic Acid Gel Staining Kit

Gelite™ Orange is an extremely sensitive nucleic acid gel stain for detecting DNA or RNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film. As with Helixyte™ Green stain, this remarkable sensitivity can be attributed to a combination of unique dye characteristics. Because the nucleic acid-bound Gelite™ Orange dye exhibits excitation maxima at both ~495 nm and ~300 nm (the emission maximum is ~537 nm), it is compatible with a wide variety of instrumentation, ranging from UV epi- and transilluminators and blue-light transilluminators, to mercury-arc lamp- and argon-ion laser-based gel scanners. Our Gelite™ Orange Nucleic Acid Gel Staining Gel Kit includes our Gelite™ Orange nucleic acid stain with an optimized and robust protocol. It provides a convenient solution for staining nucleic acid samples in gels.

160 ng of 1 kb Plus DNA Ladder  (ThermoFisher 10787018) in 0.9% agarose/TBE electrophoresis gel were stained with Gelite™ Orange and SYBR® Gold, and imaged with 254-nm UV transilluminator using UVP Bioimaging System.

Protocol

SDS

Catalog	Size	Price	Quantity
17594	1 Kit	Price

References

View all 79 references: Citation Explorer

SYBR green real-time PCR for the detection of all enterovirus-A71 genogroups

Authors:

Dubot-Peres A, Tan CY, de Chesse R, Sibounheuang B, Vongsouvath M, Phommasone K, Bessaud M, Gazin C, Thirion L, Phetsouvanh R, Newton PN, de Lamballerie X.

Journal:

PLoS One (2014): e89963

Development of loop-mediated isothermal amplification and SYBR green real-time PCR methods for the detection of Citrus yellow mosaic badnavirus in citrus species

Authors:

Anthony Johnson AM, Dasgupta I, Sai Gopal DV.

Journal:

J Virol Methods (2014): 9

Development of both multiple PCR and real-time SYBR green PCR for the detection of Vibrio cholerae non-O1/O139 serogroups

Authors:

Li F, Kan B, Wang D.

Journal:

Zhonghua Liu Xing Bing Xue Za Zhi (2014): 66

Detection of Cardamom mosaic virus and Banana bract mosaic virus in cardamom using SYBR Green based reverse transcription-quantitative PCR

Authors:

Siljo A, Bhat AI, Biju CN.

Journal:

Virusdisease (2014): 137

Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes

Authors:

Tajadini M, Panjehpour M, Javanmard SH.

Journal:

Adv Biomed Res (2014): 85

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
UNSPSC	41116134

Instrument settings

Transilluminator
Excitation	254 nm or 300 nm
Emission	Long path green filter (ex. SYBR or GelStar)

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Nucleic Acid Detection Probes & Assay Kits

Telephone
Fax
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Page updated on October 31, 2025

Component A: Gelite™ Orange Stain	1 vial (20 μL)
Component B: 5X Gel Loading Buffer	3 x 1 mL