Name: Gelite™ Orange Nucleic Acid Gel Staining Kit
Brand: AAT Bioquest
SKU: 17594
Price: 237 USD
Availability: InStock

Gelite™ Orange Nucleic Acid Gel Staining Kit

Gelite™ Orange is an extremely sensitive nucleic acid gel stain for detecting DNA or RNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film. As with Helixyte™ Green stain, this remarkable sensitivity can be attributed to a combination of unique dye characteristics. Because the nucleic acid-bound Gelite™ Orange dye exhibits excitation maxima at both ~495 nm and ~300 nm (the emission maximum is ~537 nm), it is compatible with a wide variety of instrumentation, ranging from UV epi- and transilluminators and blue-light transilluminators, to mercury-arc lamp- and argon-ion laser-based gel scanners. Our Gelite™ Orange Nucleic Acid Gel Staining Gel Kit includes our Gelite™ Orange nucleic acid stain with an optimized and robust protocol. It provides a convenient solution for staining nucleic acid samples in gels.

160 ng of 1 kb Plus DNA Ladder  (ThermoFisher 10787018) in 0.9% agarose/TBE electrophoresis gel were stained with Gelite™ Orange and SYBR® Gold, and imaged with 254-nm UV transilluminator using UVP Bioimaging System.

Protocol

SDS

Catalog	Size	Price	Quantity
17594	1 Kit	Price

References

View all 79 references: Citation Explorer

SYBR green real-time PCR for the detection of all enterovirus-A71 genogroups

Authors:

Dubot-Peres A, Tan CY, de Chesse R, Sibounheuang B, Vongsouvath M, Phommasone K, Bessaud M, Gazin C, Thirion L, Phetsouvanh R, Newton PN, de Lamballerie X.

Journal:

PLoS One (2014): e89963

Development of loop-mediated isothermal amplification and SYBR green real-time PCR methods for the detection of Citrus yellow mosaic badnavirus in citrus species

Authors:

Anthony Johnson AM, Dasgupta I, Sai Gopal DV.

Journal:

J Virol Methods (2014): 9

Development of both multiple PCR and real-time SYBR green PCR for the detection of Vibrio cholerae non-O1/O139 serogroups

Authors:

Li F, Kan B, Wang D.

Journal:

Zhonghua Liu Xing Bing Xue Za Zhi (2014): 66

Detection of Cardamom mosaic virus and Banana bract mosaic virus in cardamom using SYBR Green based reverse transcription-quantitative PCR

Authors:

Siljo A, Bhat AI, Biju CN.

Journal:

Virusdisease (2014): 137

Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes

Authors:

Tajadini M, Panjehpour M, Javanmard SH.

Journal:

Adv Biomed Res (2014): 85

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Transilluminator
Excitation	254 nm or 300 nm
Emission	Long path green filter (ex. SYBR or GelStar)

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Page updated on July 27, 2026

Component A: Gelite™ Orange Stain	1 vial (20 μL)
Component B: 5X Gel Loading Buffer	3 x 1 mL