Gelite™ Red Nucleic Acid Gel Stain *Replaced by #17706*

Spectral Properties of Gelite™ Red Nucleic Acid Gel Stain
Excitation/Emission: 538/609 nm when bound to DNA

Gelite™ Red working solution (1X):
Make 1X-3X Gelite™ Red working solution by diluting the 10,000X stock reagent into pH 7.5 - 8 buffer (e.g., TAE, TBE or TE, preferably pH 8.0). Note: 1X staining solution can also be used for post gel staining, but the sensitivity will be much improved if staining with 3X staining solution. Note: Staining solutions prepared in water are less stable than those prepared in buffer and must be used within 24 hours to ensure maximal staining sensitivity. Note: In addition, staining solutions prepared in buffers with pH below 7.5 or above 8.0 are less stable and show reduced staining efficacy.

Post-Staining Protocol

1. Run gels based on your standard protocol.
   
   
2. Place the gel in a suitable polypropylene container. Gently add a sufficient amount of the Gelite™ Red working solution to submerge the gel. Note: Do not use a glass container, as it will adsorb much of the dye in the staining solution.
   
   
3. Agitate the gel gently at room temperature for ~30 minutes, protected from the light. Note: The staining solution can be stored in the dark (preferably refrigerated) for a week and reused up to 2 - 3 times.
   
   
4. Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.

Pre-Casting Protocol

1. Prepare agarose gel solution using your standard protocol.
   
   
2. Add 1X Gelite™ Red working solutionthe gel and mix thoroughly.
   
   
3. Run gels based on your standard protocol.
   
   
4. Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.

DNA-Staining Before Electrophoresis

1. Incubate DNA with a 1:1000 to 1:3000 dilution of the dye (in TE, TBE, or TAE) for at least 15 minutes prior to electrophoresis.
   
   
2. Run gels based on your standard protocol.
   
   
3. Image the stained gel with a 254 nm transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.