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Gelite™ Red Nucleic Acid Gel Stain *Replaced by #17706*

Comparison of Gelite™ Red and SYBR™ Gold  in post  gel staining. 1 kb Plus DNA Ladder (200ng and 100ng per lane ) were subjected to electrophoresis in 0.9% agarose in 1 x TAE buffer. The gels were subsequently stained with a 1x  Gelite™ Red or SYBR™ Gold in 1x TAE for 30 minutes and photographed using ChemiDoc™ MP Imager with an EtBr filter.
Comparison of Gelite™ Red and SYBR™ Gold  in post  gel staining. 1 kb Plus DNA Ladder (200ng and 100ng per lane ) were subjected to electrophoresis in 0.9% agarose in 1 x TAE buffer. The gels were subsequently stained with a 1x  Gelite™ Red or SYBR™ Gold in 1x TAE for 30 minutes and photographed using ChemiDoc™ MP Imager with an EtBr filter.
Comparison of ethidium bromide (EtBr) and Gelite™ Red in precast gel staining using 1% agarose gel in TBE buffer. 100 ng, 50 ng, 25 ng 1 kb Plus DNA Ladder were loaded from left to right. Gels were imaged using ChemiDoc™ MP Imager with an EtBr filter.
Ordering information
Price ()Discontinued
Catalog Number17600
Unit Size
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Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


Gelite™ Red is the most recent addition to our Gelite™ nucleic acid gel stain family. Now we have a complete family of multicolor gel stains for detecting nucleic acids in gels. Gelite™ Red is an extremely sensitive nucleic acid gel stain for detecting DNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film. Under the same conditions it is more sensitive than the popular SYBR® Gold gel stain. This remarkable sensitivity can be attributed to a combination of unique dye characteristics of Gelite™ Red. Because the nucleic acid"bound Gelite™ Red dye exhibits excitation maxima close to 488 nm and ~300 nm (the emission maximum is ~610 nm), it is compatible with a wide variety of instrumentation, ranging from UV epi- and transilluminators and blue-light transilluminators, to mercury-arc lamp" and argon-ion laser"based gel scanners. Our Gelite™ Red is a superior alternative to SYBR® Gold, SYBR® Safe and Ethidium Bromide. It provides a convenient solution for staining nucleic acid samples in gels.

Example protocol


AT A GLANCE

Spectral Properties of Gelite™ Red Nucleic Acid Gel Stain
Excitation/Emission: 538/609 nm when bound to DNA

PREPARATION OF WORKING SOLUTION

Gelite™ Red working solution (1X):
Make 1X-3X Gelite™ Red working solution by diluting the 10,000X stock reagent into pH 7.5 - 8 buffer (e.g., TAE, TBE or TE, preferably pH 8.0). Note: 1X staining solution can also be used for post gel staining, but the sensitivity will be much improved if staining with 3X staining solution. Note: Staining solutions prepared in water are less stable than those prepared in buffer and must be used within 24 hours to ensure maximal staining sensitivity. Note: In addition, staining solutions prepared in buffers with pH below 7.5 or above 8.0 are less stable and show reduced staining efficacy.

SAMPLE EXPERIMENTAL PROTOCOL

Post-Staining Protocol

  1. Run gels based on your standard protocol.

  2. Place the gel in a suitable polypropylene container. Gently add a sufficient amount of the Gelite™ Red working solution to submerge the gel. Note: Do not use a glass container, as it will adsorb much of the dye in the staining solution.

  3. Agitate the gel gently at room temperature for ~30 minutes, protected from the light. Note: The staining solution can be stored in the dark (preferably refrigerated) for a week and reused up to 2 - 3 times.

  4. Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.

Pre-Casting Protocol

  1. Prepare agarose gel solution using your standard protocol.

  2. Add 1X Gelite™ Red working solutionthe gel and mix thoroughly.

  3. Run gels based on your standard protocol.

  4. Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.

DNA-Staining Before Electrophoresis

  1. Incubate DNA with a 1:1000 to 1:3000 dilution of the dye (in TE, TBE, or TAE) for at least 15 minutes prior to electrophoresis.

  2. Run gels based on your standard protocol.

  3. Image the stained gel with a 254 nm transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.

Citations


View all 14 citations: Citation Explorer
Ethidium DNA agarose gel electrophoresis: how it started
Authors: Borst, P.
Journal: IUBMB Life (2005): 745-7
Comparison of ethidium bromide-stained agarose gel electrophoresis and automated fragment analysis for evaluation of IgH gene products
Authors: Gleissner, B., Maurer, J., Sindram, A., Reinhard, R., Thiel, E.
Journal: Leuk Res (2001): 769-74
An enzymatic procedure for the purification of DNA restriction fragments without gel electrophoresis and ethidium bromide staining
Authors: Ratel, D., Dupre, I., Allamane, S., Berger, F., Verna, J. M., Benabid, A. L., Wion, D.
Journal: C R Acad Sci III (2000): 753-6
Effects of ethidium bromide on DNA loop organisation in human lymphocytes measured by anomalous viscosity time dependence and single cell gel electrophoresis
Authors: Belyaev, I. Y., Eriksson, S., Nygren, J., Torudd, J., Harms-Ringdahl, M.
Journal: Biochim Biophys Acta (1999): 348-56
Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test)
Authors: Singer, V. L., Lawlor, T. E., Yue, S.
Journal: Mutat Res (1999): 37-47
Genotyping microsatellite polymorphisms by agarose gel electrophoresis with ethidium bromide staining: application to quantitative trait loci analysis of seizure susceptibility in mice
Authors: Ferraro, T. N., Schill, J. F., Ballas, C., Mulholl and , N., Golden, G. T., Smith, G. G., Buono, R. J., Berrettini, W. H.
Journal: Psychiatr Genet (1998): 227-33
The effect of ethidium bromide on mobility of DNA fragments in agarose gel electrophoresis
Authors: Sigmon, J., Larcom, L. L.
Journal: Electrophoresis (1996): 1524-7
Fluorescent labeling of DNA with ethidium homodimer without measurable decrease in DNA mobility: application to automated gel electrophoresis apparatus
Authors: Zakharov, S. F., Garner, M. M., Chrambach, A.
Journal: Anal Biochem (1995): 195-8
Ethidium bromide staining during denaturation with glyoxal for sensitive detection of RNA in agarose gel electrophoresis
Authors: Grundemann, D., Koepsell, H.
Journal: Anal Biochem (1994): 459-61
Rapid isolation of plasmid DNA by LiCl-ethidium bromide treatment and gel filtration
Authors: Kondo, T., Mukai, M., Kondo, Y.
Journal: Anal Biochem (1991): 30-5