Gelite™ Red Nucleic Acid Gel Stain *Replaced by #17706*
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Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
H-phrase | H303, H313, H340 |
Hazard symbol | T |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R68 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41116134 |
Gelite™ Red Nucleic Acid Gel Stain *Replaced by #17707* |
Overview | ![]() ![]() |
Example protocol
AT A GLANCE
Spectral Properties of Gelite™ Red Nucleic Acid Gel Stain
Excitation/Emission: 538/609 nm when bound to DNA
PREPARATION OF WORKING SOLUTION
Gelite™ Red working solution (1X):
Make 1X-3X Gelite™ Red working solution by diluting the 10,000X stock reagent into pH 7.5 - 8 buffer (e.g., TAE, TBE or TE, preferably pH 8.0). Note: 1X staining solution can also be used for post gel staining, but the sensitivity will be much improved if staining with 3X staining solution. Note: Staining solutions prepared in water are less stable than those prepared in buffer and must be used within 24 hours to ensure maximal staining sensitivity. Note: In addition, staining solutions prepared in buffers with pH below 7.5 or above 8.0 are less stable and show reduced staining efficacy.
SAMPLE EXPERIMENTAL PROTOCOL
Post-Staining Protocol
- Run gels based on your standard protocol.
- Place the gel in a suitable polypropylene container. Gently add a sufficient amount of the Gelite™ Red working solution to submerge the gel. Note: Do not use a glass container, as it will adsorb much of the dye in the staining solution.
- Agitate the gel gently at room temperature for ~30 minutes, protected from the light. Note: The staining solution can be stored in the dark (preferably refrigerated) for a week and reused up to 2 - 3 times.
- Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.
Pre-Casting Protocol
- Prepare agarose gel solution using your standard protocol.
- Add 1X Gelite™ Red working solutionthe gel and mix thoroughly.
- Run gels based on your standard protocol.
- Image the stained gel with a 254 nm transilluminator or a laser-based gel scanner using a long path green filter, such as a SYBR® filter or GelStar® filter.
DNA-Staining Before Electrophoresis
- Incubate DNA with a 1:1000 to 1:3000 dilution of the dye (in TE, TBE, or TAE) for at least 15 minutes prior to electrophoresis.
- Run gels based on your standard protocol.
- Image the stained gel with a 254 nm transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR® filter or GelStar® filter.
Images

Citations
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