Gelite™ Red Nucleic Acid Gel Stain *Replaced by #17707*

Gelite™ Red Nucleic Acid Gel Stain

Replaced by #17707

Gelite™ Red is the most recent addition to our Gelite™ nucleic acid gel stain family. Now we have a complete family of multicolor gel stains for detecting nucleic acids in gels. Gelite™ Red is an extremely sensitive nucleic acid gel stain for detecting DNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film. Under the same conditions it is more sensitive than the popular SYBR® Gold gel stain. This remarkable sensitivity can be attributed to a combination of unique dye characteristics of Gelite™ Red. Because the nucleic acid“bound Gelite™ Red dye exhibits excitation maxima close to 488 nm and ~300 nm (the emission maximum is ~610 nm), it is compatible with a wide variety of instrumentation, ranging from UV epi- and transilluminators and blue-light transilluminators, to mercury-arc lamp“ and argon-ion laser“based gel scanners. Our Gelite™ Red is a superior alternative to SYBR® Gold, SYBR® Safe and Ethidium Bromide. It provides a convenient solution for staining nucleic acid samples in gels.

Comparison of ethidium bromide (EtBr) and Gelite™ Red in precast gel staining using 1% agarose gel in TBE buffer. 100 ng, 50 ng, 25 ng 1 kb Plus DNA Ladder (Invitrogen) were loaded from left to right. Gels were imaged using ChemiDoc™ MP Imager with an EtBr filter.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17603	10 mL	Price

Citations

View all 14 citations: Citation Explorer

Ethidium DNA agarose gel electrophoresis: how it started

Authors:

Borst, P.

Journal:

IUBMB Life (2005): 745-7

Comparison of ethidium bromide-stained agarose gel electrophoresis and automated fragment analysis for evaluation of IgH gene products

Authors:

Gleissner, B., Maurer, J., Sindram, A., Reinhard, R., Thiel, E.

Journal:

Leuk Res (2001): 769-74

An enzymatic procedure for the purification of DNA restriction fragments without gel electrophoresis and ethidium bromide staining

Authors:

Ratel, D., Dupre, I., Allamane, S., Berger, F., Verna, J. M., Benabid, A. L., Wion, D.

Journal:

C R Acad Sci III (2000): 753-6

Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test)

Authors:

Singer, V. L., Lawlor, T. E., Yue, S.

Journal:

Mutat Res (1999): 37-47

Effects of ethidium bromide on DNA loop organisation in human lymphocytes measured by anomalous viscosity time dependence and single cell gel electrophoresis

Authors:

Belyaev, I. Y., Eriksson, S., Nygren, J., Torudd, J., Harms-Ringdahl, M.

Journal:

Biochim Biophys Acta (1999): 348-56

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

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Page updated on February 1, 2023