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Glucose-UDP-(PEG)6-Fluorescein Conjugate

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Physical properties
Molecular weight1530.54
SolventWater
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
1530.54
Absorbance (nm)
487
Correction Factor (260 nm)
0.32
Correction Factor (280 nm)
0.35
Extinction coefficient (cm -1 M -1)
800001
Excitation (nm)
498
Emission (nm)
517
Quantum yield
0.79001, 0.952
Uridine-5'-diphospho-1-alpha-D-glucose (UDP-Glc) is a key intermediate in carbohydrate metabolism. UDP-Glc serves as a precursor of glycogen, can be metabolized into UDP-galactose and UDP-glucuronic acid which can then be incorporated into polysaccharides as galactose and glucuronic acid. UDP-Glc serves as a precursor of sucrose lipopolysaccharides and glycosphingolipids. UDP-Glc is a common substrate used by glucosyltransferases, including certain bacterial toxins such as Toxins A and B from Clostridium di?cile. Fluorescent analogs of UDP-Glc might be used for studying glucosyltransferases and clostridial toxins. This fluorescein UDP-Glc derivative has the spectral properties identical to those of FITC conjugates. Compared to #11705, this UDP-Glc derivative has a much longer spacer between the fluorophore and UDP moiety.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Glucose-UDP-(PEG)6-Fluorescein Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM65.336 µL326.682 µL653.364 µL3.267 mL6.534 mL
5 mM13.067 µL65.336 µL130.673 µL653.364 µL1.307 mL
10 mM6.534 µL32.668 µL65.336 µL326.682 µL653.364 µL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952

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References


View all 161 references: Citation Explorer
cDNA cloning and characterization of UDP-glucose: anthocyanidin 3-O-glucosyltransferase in Freesia hybrida
Authors: Sui X, Gao X, Ao M, Wang Q, Yang D, Wang M, Fu Y, Wang L.
Journal: Plant Cell Rep (2011): 1209
Downregulation of putative UDP-glucose: flavonoid 3-O-glucosyltransferase gene alters flower coloring in Phalaenopsis
Authors: Chen WH, Hsu CY, Cheng HY, Chang H, Chen HH, Ger MJ.
Journal: Plant Cell Rep (2011): 1007
Molecular analysis of a UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) gene from purple potato (Solanum tuberosum)
Authors: Hu C, Gong Y, Jin S, Zhu Q.
Journal: Mol Biol Rep (2011): 561
Identification of a UDP-glucose pyrophosphorylase from cotton (Gossypium hirsutum L.) involved in cellulose biosynthesis in Arabidopsis thaliana
Authors: Wang Q, Zhang X, Li F, Hou Y, Liu X.
Journal: Plant Cell Rep (2011): 1303
Molecular cloning and analysis of the UDP-Glucose Pyrophosphorylase in Streptococcus equi subsp. zooepidemicus
Authors: Ma Z, Fan HJ, Lu CP.
Journal: Mol Biol Rep (2011): 2751
Regulation of UDP-glucose dehydrogenase is sufficient to modulate hyaluronan production and release, control sulfated GAG synthesis, and promote chondrogenesis
Authors: Clarkin CE, Allen S, Kuiper NJ, Wheeler BT, Wheeler-Jones CP, Pitsillides AA.
Journal: J Cell Physiol (2011): 749
Functional analysis of a rice late pollen-abundant UDP-glucose pyrophosphorylase (OsUgp2) promoter
Authors: Huang Z, Gan Z, He Y, Li Y, Liu X, Mu H.
Journal: Mol Biol Rep (2011): 4291
An enzymatic method to distinguish tetrahydrobiopterin from oxidized biopterins using UDP-glucose:tetrahydrobiopterin glucosyltransferase
Authors: Kim HL, Kim do H, Lee YK, Park SO, Lee YW, Kwon OS, Park YS.
Journal: Anal Biochem (2010): 79
Functional characterization of a UDP-glucose:flavonoid 3-O-glucosyltransferase from the seed coat of black soybean (Glycine max (L.) Merr.)
Authors: Kovinich N, Saleem A, Arnason JT, Miki B.
Journal: Phytochemistry (2010): 1253
Trypanosoma brucei UDP-glucose:glycoprotein glucosyltransferase has unusual substrate specificity and protects the parasite from stress
Authors: Izquierdo L, Atrih A, Rodrigues JA, Jones DC, Ferguson MA.
Journal: Eukaryot Cell (2009): 230