Glucose-UDP-(PEG)6-Fluorescein Conjugate

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Physical properties

Molecular weight	1530.54
Solvent	Water

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1530.54

Absorbance (nm)

487

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.35

Extinction coefficient (cm ^-1 M ^-1)

80000¹

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.7900¹, 0.95²

Uridine-5'-diphospho-1-alpha-D-glucose (UDP-Glc) is a key intermediate in carbohydrate metabolism. UDP-Glc serves as a precursor of glycogen, can be metabolized into UDP-galactose and UDP-glucuronic acid which can then be incorporated into polysaccharides as galactose and glucuronic acid. UDP-Glc serves as a precursor of sucrose lipopolysaccharides and glycosphingolipids. UDP-Glc is a common substrate used by glucosyltransferases, including certain bacterial toxins such as Toxins A and B from Clostridium di?cile. Fluorescent analogs of UDP-Glc might be used for studying glucosyltransferases and clostridial toxins. This fluorescein UDP-Glc derivative has the spectral properties identical to those of FITC conjugates. Compared to #11705, this UDP-Glc derivative has a much longer spacer between the fluorophore and UDP moiety.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Glucose-UDP-(PEG)6-Fluorescein Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	65.336 µL	326.682 µL	653.364 µL	3.267 mL	6.534 mL
5 mM	13.067 µL	65.336 µL	130.673 µL	653.364 µL	1.307 mL
10 mM	6.534 µL	32.668 µL	65.336 µL	326.682 µL	653.364 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Images

Figure 1. Chemical structure for Glucose-UDP-(PEG)6-Fluorescein Conjugate

References

View all 161 references: Citation Explorer

cDNA cloning and characterization of UDP-glucose: anthocyanidin 3-O-glucosyltransferase in Freesia hybrida
Authors: Sui X, Gao X, Ao M, Wang Q, Yang D, Wang M, Fu Y, Wang L.
Journal: Plant Cell Rep (2011): 1209

Downregulation of putative UDP-glucose: flavonoid 3-O-glucosyltransferase gene alters flower coloring in Phalaenopsis
Authors: Chen WH, Hsu CY, Cheng HY, Chang H, Chen HH, Ger MJ.
Journal: Plant Cell Rep (2011): 1007

Molecular analysis of a UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) gene from purple potato (Solanum tuberosum)
Authors: Hu C, Gong Y, Jin S, Zhu Q.
Journal: Mol Biol Rep (2011): 561

Identification of a UDP-glucose pyrophosphorylase from cotton (Gossypium hirsutum L.) involved in cellulose biosynthesis in Arabidopsis thaliana
Authors: Wang Q, Zhang X, Li F, Hou Y, Liu X.
Journal: Plant Cell Rep (2011): 1303

Molecular cloning and analysis of the UDP-Glucose Pyrophosphorylase in Streptococcus equi subsp. zooepidemicus
Authors: Ma Z, Fan HJ, Lu CP.
Journal: Mol Biol Rep (2011): 2751

Regulation of UDP-glucose dehydrogenase is sufficient to modulate hyaluronan production and release, control sulfated GAG synthesis, and promote chondrogenesis
Authors: Clarkin CE, Allen S, Kuiper NJ, Wheeler BT, Wheeler-Jones CP, Pitsillides AA.
Journal: J Cell Physiol (2011): 749

Functional analysis of a rice late pollen-abundant UDP-glucose pyrophosphorylase (OsUgp2) promoter
Authors: Huang Z, Gan Z, He Y, Li Y, Liu X, Mu H.
Journal: Mol Biol Rep (2011): 4291

An enzymatic method to distinguish tetrahydrobiopterin from oxidized biopterins using UDP-glucose:tetrahydrobiopterin glucosyltransferase
Authors: Kim HL, Kim do H, Lee YK, Park SO, Lee YW, Kwon OS, Park YS.
Journal: Anal Biochem (2010): 79

Functional characterization of a UDP-glucose:flavonoid 3-O-glucosyltransferase from the seed coat of black soybean (Glycine max (L.) Merr.)
Authors: Kovinich N, Saleem A, Arnason JT, Miki B.
Journal: Phytochemistry (2010): 1253

Trypanosoma brucei UDP-glucose:glycoprotein glucosyltransferase has unusual substrate specificity and protects the parasite from stress
Authors: Izquierdo L, Atrih A, Rodrigues JA, Jones DC, Ferguson MA.
Journal: Eukaryot Cell (2009): 230