Name: Gly-Phe-2-naphthylamide
Brand: AAT Bioquest
SKU: 20054
Price: 77 USD
Availability: InStock

Gly-Phe-2-naphthylamide

Gly-Phe-2-naphthylamide is a dipeptide substrate for Cathepsin C that releases fluorescent 2-naphthylamine upon enzymatic cleavage, used for measuring lysosomal cysteine protease activity in biochemical assays.

Selective for Cathepsin C: Targeted substrate for studying Cathepsin C activity
Lysosomal Dynamics: Enables research on lysosomal membrane integrity and function
Apoptosis Tool: Inhibits Cathepsin C-dependent caspase-8 activation, useful for studying protease-mediated cell death
Lysosomal Membrane Disruption: Cleavage products compromise lysosomal membrane integrity
Broad Application: Suitable for assays on protease activity, lysosomal function, and apoptosis

Catalog	Size	Price	Quantity
20054	10 mg	Price

Overview

Gly-Phe-2-naphthylamide is a selective substrate for Cathepsin C, a lysosomal cysteine protease that catalyzes the sequential removal of dipeptides from various proteins and polypeptides. This compound is widely employed in biochemical assays to investigate intralysosomal hydrolysis, assess lysosomal membrane integrity, and explore the functional role of Cathepsin C in cellular processes. Upon hydrolysis by Cathepsin C, Gly-Phe-2-naphthylamide is cleaved into products that are retained within the lysosome, leading to disruption of lysosomal membrane integrity. Gly-Phe-2-naphthylamide is particularly useful in studies of proteolytic activity and lysosomal dynamics, as well as apoptosis research. At a concentration of 50 μM, Gly-Phe-2-naphthylamide has been demonstrated to inhibit the Cathepsin C-mediated activation of caspase-8, making it a critical tool for dissecting the molecular mechanisms involved in programmed cell death. Its ability to impair lysosomal membrane integrity and modulate caspase-8 activity underscores its utility in studies of lysosomal function, protease activity, and apoptotic signaling pathways, particularly in the context of protease-mediated apoptosis.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Gly-Phe-2-naphthylamide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	287.836 µL	1.439 mL	2.878 mL	14.392 mL	28.784 mL
5 mM	57.567 µL	287.836 µL	575.672 µL	2.878 mL	5.757 mL
10 mM	28.784 µL	143.918 µL	287.836 µL	1.439 mL	2.878 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	347.42 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Two-pore channel 1 and Ca2+ release-activated Ca2+ channels contribute to the acrosomal pH-dependent intracellular Ca2+ increase in mouse sperm.

Authors:

Oliver, Enrique I and Jabloñski, Martina and Buffone, Mariano G and Darszon, Alberto

Journal:

The Journal of physiology (2023): 2935-2958

LRRK2 is required for CD38-mediated NAADP-Ca2+ signaling and the downstream activation of TFEB (transcription factor EB) in immune cells.

Authors:

Nabar, Neel R and Heijjer, Christopher N and Shi, Chong-Shan and Hwang, Il-Young and Ganesan, Sundar and Karlsson, Mikael C I and Kehrl, John H

Journal:

Autophagy (2022): 204-222

Nicotinic Acid Adenine Dinucleotide Phosphate Induces Intracellular Ca2+ Signalling and Stimulates Proliferation in Human Cardiac Mesenchymal Stromal Cells.

Authors:

Faris, Pawan and Casali, Claudio and Negri, Sharon and Iengo, Lara and Biggiogera, Marco and Maione, Angela Serena and Moccia, Francesco

Journal:

Frontiers in cell and developmental biology (2022): 874043

Nicotinic acid adenine dinucleotide phosphate activates two-pore channel TPC1 to mediate lysosomal Ca2+ release in endothelial colony-forming cells.

Authors:

Moccia, Francesco and Zuccolo, Estella and Di Nezza, Francesca and Pellavio, Giorgia and Faris, Pawan S and Negri, Sharon and De Luca, Antonio and Laforenza, Umberto and Ambrosone, Luigi and Rosti, Vittorio and Guerra, Germano

Journal:

Journal of cellular physiology (2021): 688-705

Vitamin D3 activates the autolysosomal degradation function against Helicobacter pylori through the PDIA3 receptor in gastric epithelial cells.

Authors:

Hu, Wei and Zhang, Lin and Li, Ming Xing and Shen, Jing and Liu, Xiao Dong and Xiao, Zhan Gang and Wu, Ding Lan and Ho, Idy H T and Wu, Justin C Y and Cheung, Cynthia K Y and Zhang, Yu Chen and Lau, Alaster H Y and Ashktorab, Hassan and Smoot, Duane T and Fang, Evandro F and Chan, Matthew T V and Gin, Tony and Gong, Wei and Wu, William K K and Cho, Chi Hin

Journal:

Autophagy (2019): 707-725

Physical properties

Molecular weight	347.42
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	21438-66-4

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis

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Page updated on December 30, 2025