Gly-Pro-AMC *CAS 115035-46-6*

Gly-Pro-AMC

CAS 115035-46-6

Gly-Pro-AMC is a sensitive fluorogenic substrate for detecting dipeptidyl peptidase IV (DPPIV), a serine protease that cleaves N-terminal dipeptides from polypeptides with L-proline or L-alanine at the penultimate position. DPPIV is a multifunctional protein expressed on the surface of several cell types including epithelial, endothelial and lymphoid cells. It is identical to the T cell activation antigen CD26 and the adenosine deaminase binding protein, and it is also released as a soluble form in plasma. The substrates of CD26/DPPIV include a wide variety of proline-containing peptides such as growth factors, chemokines, neuropeptides, and vasoactive peptides. DPPIV is involved in immune regulation, signal transduction, and apoptosis, and appears to play an important role in tumor progression. Importantly, DPPIV is a therapeutic target for type II diabetes due to its role as a serum protease that cleaves incretin hormones of the glucagon family of peptides and thus regulates glucose homeostasis. Studies indicate that a DPPIV inhibitor improves impaired glucose tolerance. The assay of DPPIV with Gly-Pro-AMC is a simple, fast and flexible assay that is ideal for high-through put screening. The assay demonstrates improved sensitivity compared to colorimetric DPPIV assays, allowing the researcher to use less enzyme while still achieving appropriate Z' values. The homogeneous coupled-enzyme format is convenient, requiring only a single reagent addition to the test samples. Maximal sensitivity is achieved in 20-30 minutes, and the signal generated is very stable.

Catalog	Size	Price	Quantity
13450	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Gly-Pro-AMC *CAS 115035-46-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	225.545 µL	1.128 mL	2.255 mL	11.277 mL	22.555 mL
5 mM	45.109 µL	225.545 µL	451.091 µL	2.255 mL	4.511 mL
10 mM	22.555 µL	112.773 µL	225.545 µL	1.128 mL	2.255 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	443.37 g/mol	=	mL	x	mM	=	mmol

Citations

View all 9 citations: Citation Explorer

Synthesis, Radiolabeling, and Evaluation of a Dimeric Fibroblast Activation Protein Inhibitor with a Triazine Linker

Authors:

Zhang, Xuran and Kang, Choong Mo and Choi, Joon Young and Choe, Yearn Seong

Journal:

Molecular Pharmaceutics (2025)

Activation of the NLRP1 Inflammasome and Its Role in Transmissible Gastroenteritis Coronavirus Infection

Authors:

He, Haojie and Wang, Wenzhe and Li, Liang and Zhang, Xin and Shi, Hongyan and Chen, Jianfei and Shi, Da and Xue, Mei and Feng, Li

Journal:

Journal of Virology (2023): e00589--23

Manipulation of saliva-derived microcosm biofilms to resemble dysbiotic subgingival microbiota

Authors:

Jiang, Yaling and Brandt, Bernd W and Buijs, Mark J and Cheng, Lei and Exterkate, Rob AM and Crielaard, Wim and Deng, Dong Mei

Journal:

Applied and Environmental Microbiology (2021): e02371--20

Collagen peptides with DPP-IV inhibitory activity from sheep skin and their stability to in vitro gastrointestinal digestion

Authors:

Wang, Beibei and Yu, Zhe and Yokoyama, Wallace and Chiou, Bor-Sen and Chen, Maoshen and Liu, Fei and Zhong, Fang

Journal:

Food Bioscience (2021): 101161

Characterization of DPP-IV Inhibitory Peptides Using an In Vitro Cell Culture Model of the Intestine

Authors:

Jin, Ritian and Shang, Jiaqi and Teng, Xiangyu and Zhang, Ligang and Liao, Minhe and Kang, Jiaxin and Meng, Ran and Wang, Dangfeng and Ren, Haowei and Liu, Ning

Journal:

Journal of Agricultural and Food Chemistry (2021): 2711--2718

References

View all 7 references: Citation Explorer

Slow-binding inhibition of peptide deformylase by cyclic peptidomimetics as revealed by a new spectrophotometric assay

Authors:

Nguyen KT, Hu X, Pei D.

Journal:

Bioorg Chem (2004): 178

Activity of DPP III in human cerebrospinal fluid derived from patients with pain

Authors:

Sato H, Kimura K, Yamamoto Y, Hazato T.

Journal:

Masui (2003): 257

Extracellular peptidases of imaginal discs of Drosophila melanogaster

Authors:

Wilson CL, Shirras AD, Isaac RE.

Journal:

Peptides (2002): 2007

Dipeptidyl peptidase I: importance of progranzyme activation sequences, other dipeptide sequences, and the N-terminal amino group of synthetic substrates for enzyme activity

Authors:

Tran TV, Ellis KA, Kam CM, Hudig D, Powers JC.

Journal:

Arch Biochem Biophys (2002): 160

Purification and characterization of barley dipeptidyl peptidase IV

Authors:

Davy A, Thomsen KK, Juliano MA, Alves LC, Svendsen I, Simpson DJ.

Journal:

Plant Physiol (2000): 425

Physical properties

Molecular weight	443.37
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	115035-46-6

Instrument settings

Fluorescence microplate reader
Excitation	380 nm
Emission	500 nm
Cutoff	435 nm
Recommended plate	Solid black

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Page updated on October 7, 2025