Name: GTIF488 [iFluor® 488 gentamicin conjugate]
Brand: AAT Bioquest
SKU: 24302
Availability: InStock

GTIF488

iFluor® 488 gentamicin conjugate

GTIF488 is a green fluorescent gentamicin conjugate for studying cellular uptake, trafficking, and distribution of gentamicin using fluorescence-based techniques.

Fluorescent Gentamicin Analog: Enables visualization of gentamicin uptake and intracellular distribution.
Spectral Properties: Excitation at 491 nm and emission at 516 nm.
Instrument Compatibility: Supports 488 nm laser excitation and FITC filter sets.
Cochlear Transport Studies: May be used to investigate gentamicin trafficking across the blood-labyrinth barrier into hair cells.
Applications: Suitable for fluorescence microscopy, fluorescence correlation spectroscopy, and single-molecule spectroscopy.

Fluorophore-tagged drugs, such as gentamicin- iFluor® 488 (GTIF488) conjugates, might be used to identify drug trafficking routes across the BLB and into hair cells, and specifically in the cochlea - the sensory organ responsible for hearing.

Datasheet

COA

Catalog	Size	Price	Quantity
24302	100 ug	Price
24303	1 mg	Price

Overview

Gentamicin is widely used for treating tuberculosis and Gram-negative infections and is particularly useful in neonatal intensive care units. Fluorophore-tagged drugs, such as gentamicin- iFluor® 488 (GTIF488) conjugates, might be used to identify drug trafficking routes across the BLB and into hair cells, and specifically in the cochlea - the sensory organ responsible for hearing. Identifying the mechanisms involved in the intracochlear trafficking of ototoxic drugs to hair cells is of fundamental as well as clinical importance. GTIF488 is well excited with the common Argon laser at 488 nm. It provides researchers a valuable tool to study the cellular uptake, kinetics and distribution of gentamicin through fluorescence techniques, such as fluorescence microscopy, fluorescence correlation spectroscopy, single molecule spectroscopy. The iFluor® 488 is well compatible with the common FITC filter set.

References

View all 50 references: Citation Explorer

In vivo real-time imaging reveals megalin as the aminoglycoside gentamicin transporter into cochlea whose inhibition is otoprotective.

Authors:

Kim, Jinkyung and Ricci, Anthony J

Journal:

Proceedings of the National Academy of Sciences of the United States of America (2022)

Identification of a series of hair-cell MET channel blockers that protect against aminoglycoside-induced ototoxicity.

Authors:

Kenyon, Emma J and Kirkwood, Nerissa K and Kitcher, Siân R and Goodyear, Richard J and Derudas, Marco and Cantillon, Daire M and Baxendale, Sarah and de la Vega de León, Antonio and Mahieu, Virginia N and Osgood, Richard T and Wilson, Charlotte Donald and Bull, James C and Waddell, Simon J and Whitfield, Tanya T and Ward, Simon E and Kros, Corné J and Richardson, Guy P

Journal:

JCI insight (2021)

Hair cell uptake of gentamicin in the developing mouse utricle.

Authors:

Qian, Xiaoqing and He, Ziyu and Wang, Yanmei and Chen, Binjun and Hetrick, Alisa and Dai, Chunfu and Chi, Fanglu and Li, Hongzhe and Ren, Dongdong

Journal:

Journal of cellular physiology (2020)

Systemic Fluorescent Gentamicin Enters Neonatal Mouse Hair Cells Predominantly Through Sensory Mechanoelectrical Transduction Channels.

Authors:

Makabe, Ayane and Kawashima, Yoshiyuki and Sakamaki, Yuriko and Maruyama, Ayako and Fujikawa, Taro and Ito, Taku and Kurima, Kiyoto and Griffith, Andrew J and Tsutsumi, Takeshi

Journal:

Journal of the Association for Research in Otolaryngology : JARO (2020): 137-149

A new aminoglycoside etimicin shows low nephrotoxicity and ototoxicity in zebrafish embryos.

Authors:

Shao, Weihao and Zhong, Dan and Jiang, Haowei and Han, Yujie and Yin, Yu and Li, Ruining and Qian, Xiuping and Chen, Daijie and Jing, Lili

Journal:

Journal of applied toxicology : JAT (2020)

Physical properties

Molecular weight	~1100
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 25, 2026