Name: GTIF647 [iFluor® 647 gentamicin conjugate]
Brand: AAT Bioquest
SKU: 24304
Availability: InStock

GTIF647

iFluor® 647 gentamicin conjugate

GTIF647 is a far-red fluorescent gentamicin conjugate for studying cellular uptake, trafficking, and distribution of gentamicin using fluorescence-based techniques.

Fluorescent Gentamicin Analog: Enables visualization of gentamicin uptake and cellular distribution.
Spectral Properties: Excitation at 655 nm and emission at 670 nm.
Instrument Compatibility: Supports 632 nm and 647 nm laser excitation and Cy5 filter sets.
Cochlear Transport Studies: May be used to investigate gentamicin trafficking across the blood-labyrinth barrier into cochlear hair cells.
Applications: Suitable for fluorescence microscopy, fluorescence correlation spectroscopy, and single-molecule spectroscopy.

Gentamicin is widely used for treating tuberculosis and Gram-negative infections and is particularly useful in neonatal intensive care units. Fluorophore-tagged drugs, such as gentamicin- iFluor® 647 (GTIF647) conjugates, might be used to identify drug trafficking routes across the BLB and into hair cells, and specifically in the cochlea.

Datasheet

COA

Catalog	Size	Price	Quantity
24304	100 ug	Price
24305	1 mg	Price

Overview

Gentamicin is widely used for treating tuberculosis and Gram-negative infections and is particularly useful in neonatal intensive care units. Fluorophore-tagged drugs, such as gentamicin- iFluor® 647 (GTIF647) conjugates, might be used to identify drug trafficking routes across the BLB and into hair cells, and specifically in the cochlea - the sensory organ responsible for hearing. Identifying the mechanisms involved in the intracochlear trafficking of ototoxic drugs to hair cells is of fundamental as well as clinical importance. GTIF647 is well excited with the popular red laser at 647 nm or HeNe laser at 632 nm. It provides researchers a valuable tool to study the cellular uptake, kinetics and distribution of gentamicin through fluorescence techniques, such as fluorescence microscopy, fluorescence correlation spectroscopy, single molecule spectroscopy. The iFluor® 647 is well compatible with the common Cy5 filter set.

References

View all 50 references: Citation Explorer

Fluorescence-Based Binding Characterization of Small Molecule Ligands Targeting CUG RNA Repeats.

Authors:

Chang, Zhihua and Zheng, Ya Ying and Mathivanan, Johnsi and Valsangkar, Vibhav A and Du, Jinxi and Abou-Elkhair, Reham A I and Hassan, Abdalla E A and Sheng, Jia

Journal:

International journal of molecular sciences (2022)

Liquid chromatography-tandem mass spectrometry method for quantification of gentamicin and its individual congeners in serum and comparison results with two immunoanalytical methods (fluorescence polarization immunoassay and chemiluminiscent microparticle

Authors:

Brozmanova, Hana and Urinovska, Romana and Safarcik, Kristian and Vsiansky, Frantisek and Kacirova, Ivana and Grundmann, Milan

Journal:

Clinica chimica acta; international journal of clinical chemistry (2021): 191-198

Development of a green fluorescence protein (GFP)-based bioassay for detection of antibiotics and its application in milk.

Authors:

Yazgan Karacaglar, Nazife Nur and Topcu, Ali and Dudak, Fahriye Ceyda and Boyaci, Ismail Hakki

Journal:

Journal of food science (2020): 500-509

Micronomicin/tobramycin binding with DNA: fluorescence studies using of ethidium bromide as a probe and molecular docking analysis.

Authors:

Bi, Shuyun and Zhou, Huifeng and Wu, Jun and Sun, Xiaoyue

Journal:

Journal of biomolecular structure & dynamics (2019): 1464-1476

Label-free and nicking enzyme-assisted fluorescence signal amplification for RNase H determination based on a G-quadruplexe/thioflavin T complex.

Authors:

Wu, Kefeng and Ma, Changbei and Deng, Zhiyi and Fang, Ning and Tang, Zhenwei and Zhu, Xingxing and Wang, Kemin

Journal:

Talanta (2018): 142-147

Physical properties

Molecular weight	~1200
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	655
Emission (nm)	670
Quantum yield	0.25 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 25, 2026