Name: GTTR [Texas Red gentamicin conjugate]
Brand: AAT Bioquest
SKU: 24300
Availability: InStock

GTTR

Texas Red gentamicin conjugate

Gentamicin is widely used for treating tuberculosis and Gram-negative infections and is particularly useful in neonatal intensive care units. Fluorophore-tagged drugs, such as gentamicin-Texas Red (GTTR) conjugates, have been used to identify drug trafficking routes across the BLB and into hair cells, and specifically in the cochlea - the sensory organ responsible for hearing. Identifying the mechanisms involved in the intracochlear trafficking of ototoxic drugs to hair cells is of fundamental as well as clinical importance. Texas Red-gentamicin conjugate (GTTR) provides researchers a valuable tool to study the cellular uptake, kinetics and distribution of gentamicin through fluorescence techniques, such as fluorescence microscopy, fluorescence correlation spectroscopy, single molecule spectroscopy. The TR fluorophore can be conveniently excited from 500-600 nm, with an emission maximum centered around 610 nm. Acute nephrotoxicity and permanent ototoxicity are serious side-effects that increase patient morbidity, or induce permanent auditory and vestibular deficits. Several approaches have been used to study the intracellular mechanisms induced by ototoxic drugs. However, ototoxic drugs must first cross the blood-labyrinth barrier (BLB) before entering sensory hair cells to exert their cytotoxic effect that leads to hearing loss and deafness. The BLB is similar to the blood-brain barrier (BBB), and is composed of tight junction-coupled endothelial and epithelial barrier layers that transport nutrients and ions from one side of the barrier to the other.

Protocol

COA

Catalog	Size	Price	Quantity
24300	100 ug	Price
24301	1 mg	Price

Citations

View all 5 citations: Citation Explorer

Bioelectronic drug-free control of opportunistic pathogens through selective excitability

Authors:

Kim, Saehyun and Eig, Ethan and Yue, Jiping and Yang, Albert and Comerci, Colin J and Laune, Megan and Yang, Chuanwang and Kamath, Ananth and Shi, Jiuyun and Li, Pengju and others,

Journal:

Device (2024)

Genetics of cystogenesis in base-edited human organoids reveal therapeutic strategies for polycystic kidney disease

Authors:

Vishy, Courtney E and Thomas, Chardai and Vincent, Thomas and Crawford, Daniel K and Goddeeris, Matthew M and Freedman, Benjamin S

Journal:

Cell Stem Cell (2024): 537--553

Fluid Shear Stress-Induced Changes in Megalin Trafficking Enhance Endocytic Capacity in Proximal Tubule Cells

Authors:

Lackner, Emily M and Cowan, Isabella A and Long, Kimberly R and Weisz, Ora A and Shipman, Katherine E

Journal:

bioRxiv (2024): 2024--02

Gentamicin administration leads to synaptic dysfunction in inner hair cells

Authors:

Li, Gen and Gao, Yunge and Wu, Hao and Zhao, Ting

Journal:

Toxicology Letters (2023)

Susceptibility of immature spiral ganglion neurons to aminoglycoside-induced ototoxicity is mediated by the TRPV1 channel in mice

Authors:

Bai, Yijiang and Liu, Jing and Wu, Xuewen and Pang, Bo and Zhang, Shuai and Jiang, Mengzhu and Chen, Anhai and Huang, Huping and Chen, Yongjia and Zeng, Yuan and others,

Journal:

Hearing Research (2023): 108910

References

View all 50 references: Citation Explorer

Development of a green fluorescence protein (GFP)-based bioassay for detection of antibiotics and its application in milk.

Authors:

Yazgan Karacaglar, Nazife Nur and Topcu, Ali and Dudak, Fahriye Ceyda and Boyaci, Ismail Hakki

Journal:

Journal of food science (2020): 500-509

Micronomicin/tobramycin binding with DNA: fluorescence studies using of ethidium bromide as a probe and molecular docking analysis.

Authors:

Bi, Shuyun and Zhou, Huifeng and Wu, Jun and Sun, Xiaoyue

Journal:

Journal of biomolecular structure & dynamics (2019): 1464-1476

Label-free and nicking enzyme-assisted fluorescence signal amplification for RNase H determination based on a G-quadruplexe/thioflavin T complex.

Authors:

Wu, Kefeng and Ma, Changbei and Deng, Zhiyi and Fang, Ning and Tang, Zhenwei and Zhu, Xingxing and Wang, Kemin

Journal:

Talanta (2018): 142-147

Gentamicin differentially alters cellular metabolism of cochlear hair cells as revealed by NAD(P)H fluorescence lifetime imaging.

Authors:

Zholudeva, Lyandysha V and Ward, Kristina G and Nichols, Michael G and Smith, Heather Jensen

Journal:

Journal of biomedical optics (2015): 051032

Fluorescence microscopy methods for determining the viability of bacteria in association with mammalian cells.

Authors:

Johnson, M Brittany and Criss, Alison K

Journal:

Journal of visualized experiments : JoVE (2013)

Physical properties

Molecular weight	~1200
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.23
Correction factor (280 nm)	0.36
Correction factor (565 nm)	0.4472
Extinction coefficient (cm ^-1 M ^-1)	116000 ¹
Excitation (nm)	586
Emission (nm)	603
Quantum yield	0.97 ¹ , 0.93 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet (Catalog 24300)
Safety Data Sheet (Catalog 24301)
Certificate of Analysis

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Page updated on October 22, 2025