Helix Fluor™ 545, succinimidyl ester
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1173.57 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 526 |
Emission (nm) | 543 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Helix Fluor™ 6-JOE Phosphoramidite |
Overview | SDSProtocol |
See also: Amine Reactive Dyes and Probes for Conjugation, Peptide and Oligonucleotide Labeling, Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Purification & Analysis, PCR Detection of Viral DNA/RNA
Molecular weight 1173.57 | Excitation (nm) 526 | Emission (nm) 543 |
FAM, TET, VIC, JOE, NED, TAMRA and ROX fluorescent dyes are frequently used for labeling oliogs. These fluorescent oligo conjugates are often used for nucleic acid sequencing and nucleic acid amplification. Our Helix Fluor™ 545 is an excellent alternative to the VIC dye for preparing fluorescent oligos as they have almost identical spectral properties. Compared to VIC, Helix Fluor™ 545 is much more water-soluble.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Helix Fluor™ 545, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 85.21 µL | 426.05 µL | 852.101 µL | 4.261 mL | 8.521 mL |
5 mM | 17.042 µL | 85.21 µL | 170.42 µL | 852.101 µL | 1.704 mL |
10 mM | 8.521 µL | 42.605 µL | 85.21 µL | 426.05 µL | 852.101 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Images
Figure 1. Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
Citations
View all 1 citations: Citation Explorer
Synthesis of Phytosulfokine Analogs as Probes for Studying Plant Signaling and Molecular Trafficking
Authors: Ntim, Thomas
Journal: (2021)
Authors: Ntim, Thomas
Journal: (2021)
References
View all 1 references: Citation Explorer
Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058