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Helix Fluor™ 545, succinimidyl ester

Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
Ordering information
Price ()
Catalog Number250
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1173.57
SolventDMSO
Spectral properties
Excitation (nm)526
Emission (nm)543
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
1173.57
Excitation (nm)
526
Emission (nm)
543
FAM, TET, VIC, JOE, NED, TAMRA and ROX fluorescent dyes are frequently used for labeling oliogs. These fluorescent oligo conjugates are often used for nucleic acid sequencing and nucleic acid amplification. Our Helix Fluor™ 545 is an excellent alternative to the VIC dye for preparing fluorescent oligos as they have almost identical spectral properties. Compared to VIC, Helix Fluor™ 545 is much more water-soluble.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Helix Fluor™ 545, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM85.21 µL426.05 µL852.101 µL4.261 mL8.521 mL
5 mM17.042 µL85.21 µL170.42 µL852.101 µL1.704 mL
10 mM8.521 µL42.605 µL85.21 µL426.05 µL852.101 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Excitation (nm)526
Emission (nm)543

Product family


NameExcitation (nm)Emission (nm)
Helix Fluor™ 575, succinimidyl ester553570

References


View all 1 references: Citation Explorer
Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058