Helix Fluor™ 545, succinimidyl ester

FAM, TET, VIC, JOE, NED, TAMRA and ROX fluorescent dyes are frequently used for labeling oliogs. These fluorescent oligo conjugates are often used for nucleic acid sequencing and nucleic acid amplification. Our Helix Fluor™ 545 is an excellent alternative to the VIC dye for preparing fluorescent oligos as they have almost identical spectral properties. Compared to VIC, Helix Fluor™ 545 is much more water-soluble.

<p>Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
250	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Helix Fluor™ 545, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	85.21 µL	426.05 µL	852.101 µL	4.261 mL	8.521 mL
5 mM	17.042 µL	85.21 µL	170.42 µL	852.101 µL	1.704 mL
10 mM	8.521 µL	42.605 µL	85.21 µL	426.05 µL	852.101 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1173.57 g/mol	=	mL	x	mM	=	mmol

Physical properties

Molecular weight	1173.57
Solvent	DMSO

Spectral properties

Excitation (nm)	526
Emission (nm)	543

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 12, 2025