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Helix Fluor™ 545, succinimidyl ester
FAM, TET, VIC, JOE, NED, TAMRA and ROX fluorescent dyes are frequently used for labeling oliogs. These fluorescent oligo conjugates are often used for nucleic acid sequencing and nucleic acid amplification. Our Helix Fluor™ 545 is an excellent alternative to the VIC dye for preparing fluorescent oligos as they have almost identical spectral properties. Compared to VIC, Helix Fluor™ 545 is much more water-soluble.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Helix Fluor™ 545, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 85.21 µL | 426.05 µL | 852.101 µL | 4.261 mL | 8.521 mL |
| 5 mM | 17.042 µL | 85.21 µL | 170.42 µL | 852.101 µL | 1.704 mL |
| 10 mM | 8.521 µL | 42.605 µL | 85.21 µL | 426.05 µL | 852.101 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Helix Fluor™ 575, succinimidyl ester | 553 | 570 |
Citations
View all 1 citations: Citation Explorer
Synthesis of Phytosulfokine Analogs as Probes for Studying Plant Signaling and Molecular Trafficking
Authors: Ntim, Thomas
Journal: (2021)
Authors: Ntim, Thomas
Journal: (2021)
References
View all 1 references: Citation Explorer
Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058
Page updated on October 22, 2024
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