Helix Fluor™ 575, succinimidyl ester

FAM, TET, VIC, JOE, NED, TAMRA and ROX fluorescent dyes are frequently used for labeling oliogs. These fluorescent oligo conjugates are often used for nucleic acid sequencing and nucleic acid amplification. Our Helix Fluor™ 575 is an excellent alternative to the NED dye for preparing fluorescent oligos.

<p>Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
251	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Helix Fluor™ 575, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	129.818 µL	649.089 µL	1.298 mL	6.491 mL	12.982 mL
5 mM	25.964 µL	129.818 µL	259.636 µL	1.298 mL	2.596 mL
10 mM	12.982 µL	64.909 µL	129.818 µL	649.089 µL	1.298 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	770.31 g/mol	=	mL	x	mM	=	mmol

Physical properties

Molecular weight	770.31
Solvent	DMSO

Spectral properties

Excitation (nm)	553
Emission (nm)	570

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025