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Helixyte™ Fluorimetric RNA Quantification Kit *20-1000 ng Broad Range*
Product key features
- Broad range RNA detection: Accurately quantifies RNA in the 20–1000 ng range using a fluorescence-based assay.
- High specificity: Selectively binds to RNA with minimal background from DNA, proteins, or other contaminants.
- High-capacity format: Provides reagents for up to 500 tests—ideal for high-throughput or routine lab workflows.
- Streamlined workflow: Ready-to-use reagents and a simple protocol enable quick, consistent RNA quantification.
Product description
This kit provides reagents sufficient for 500 assays, making it an ideal choice for labs conducting frequent RNA analysis or processing large sample volumes. Compatible with various RNA types, including total RNA and mRNA, Helixyte™ Fluorimetric RNA Quantification Kit simplifies quantification with its ready-to-use format and straightforward protocol. It reduces hands-on time, ensures reproducible results, and supports a wide range of downstream applications such as RT-qPCR, RNA-seq, or cDNA synthesis.
Example protocol
AT A GLANCE
- Add Helixyte™ Red RNA BR reagent working solution (200 µL).
- Add test samples (10 µL).
- Incubate at room temperature for 5 minutes.
- Monitor fluorescence intensity at Ex/Em = 643/675 nm.
Important: The following protocol is provided as an example for quantifying total RNA with Helixyte™ Red RNA BR. Warm all the components to room temperature before opening. No data are available for the mutagenicity or toxicity of Helixyte™ Red RNA BR stain. Because this reagent binds to nucleic acids, it should be treated as a potential mutagen and handled with appropriate care. The DMSO stock solution should be handled with particular caution as DMSO is known to facilitate the entry of organic molecules into tissues.
PREPARATION OF STANDARD SOLUTIONS
https://www.aatbio.com/tools/serial-dilution/17698
PREPARATION OF WORKING SOLUTION
Add 2.5 μL Helixyte™ Red RNA BR (Component A) into 1 mL of RNA Assay Buffer (Component B) and mix well. Protect the working solution from light by covering it with foil or placing it in the dark.
Note: 1 mL of working solution is enough for 5 tests.
Note: We recommend preparing this solution in a plastic container rather than glass, as the dye may adsorb to glass surfaces. For best results, this solution should be used within a few hours of its preparation.
SAMPLE EXPERIMENTAL PROTOCOL
Table 1. Layout of RNA standards and test samples in a solid black 96-well microplate. RS=RNA Standards (RS1-RS8, 1000 to 20 ng/well); BL=Blank Control; TS=Test Samples.
| BL | BL | RS8 | RS8 |
| RS1 | RS1 | TS | TS |
| RS2 | RS2 | TS | TS |
| RS3 | RS3 | ||
| RS4 | RS4 | ||
| RS5 | RS5 | ||
| RS6 | RS6 | ||
| RS7 | RS7 |
Table 2. Reagent composition for each well.
| Well | Volume | Reagent |
| RS1-RS8 | 10 µL | Serial Dilutions (1000 to 20 ng/well) |
| BL | 10 µL | Assay buffer |
| TS | 10 µL | Test Sample |
- Add 200 μL of dye working solution to each well of RNA standard, blank control, and test samples. For a 384-wellplate, add 100 μL of dye working solution into each well instead.
- Prepare RNA standards (RS), blank controls (BL), and test samples (TS) according to the layout provided in Tables 1 and 2. For a 384-well plate, use 5 μL of RNA standards or test samples per well instead of 10 μL.
- Incubate the reaction at room temperature for 5 minutes, protected from light.
- Monitor the fluorescence intensity with a fluorescence plate reader at Ex/Em = 640/675 nm (cut-off at 665 nm).
References
Authors: Tanaka, Ami and Matsubayashi, Keiji and Odajima, Takeshi and Sakata, Hidekatsu and Iida, Juri and Kai, Kazuhiro and Goto, Naoko and Satake, Masahiro
Journal: Transfusion (2024): 335-347
Authors: Dyer, Wayne B and Suzuki, Kazuo and Levert, Angelique and Starr, Mitchell and Lloyd, Andrew R and Zaunders, John J
Journal: Frontiers in immunology (2024): 1382711
Authors: Hosokawa, Kazuo and Ohmori, Hitoshi
Journal: Biochemical and biophysical research communications (2024): 151070
Authors: Qureshi, Huma and Duran, Ana Carrasco and Mahmood, Hassan and Sarwar, Zahida and Mahmood, Khalid and Midde, Krishna and Canchola, Jesse A and Parkin, Neil T and La Brot, Benjamin
Journal: Journal of viral hepatitis (2024): 156-160
Authors: Kang, Brian and Zhang, Jiayu and Schwoerer, Michael P and Nelson, Amy N and Schoeman, Emily and Guo, Andrew and Ploss, Alexander and Myhrvold, Cameron
Journal: bioRxiv : the preprint server for biology (2023)
Safety data sheet