Helixyte™ Fluorimetric RNA Quantification Kit 5-100 ng High Sensitivity

Product key features

Ultra-sensitive detection: Precisely quantifies RNA in the 5–100 ng range using a fluorescence-based assay.
High specificity: Selectively binds to RNA with minimal interference from DNA, proteins, or free nucleotides.
High-capacity format: Includes reagents for up to 500 tests—ideal for high-throughput and routine workflows.
User-friendly format: Ready-to-use reagents and a simple, fast workflow reduce hands-on time and improve consistency.

Product description

The Helixyte™ Fluorimetric RNA Quantification Kit – High Sensitivity is designed for accurate and ultra-sensitive measurement of RNA at very low concentrations. Ideal for precious or limited RNA samples, this kit uses an advanced RNA-selective fluorescent dye to achieve high specificity and minimal background noise, offering a clear advantage over traditional UV absorbance methods.

Helixyte™ Fluorimetric RNA Quantification Kit is a high-capacity format providing reagents sufficient for 500 tests. It provides reliable quantification across a variety of sample types like total RNA, mRNA, or small RNA fragments. Its streamlined protocol and ready-to-use format make it easy to integrate into existing workflows, saving time and giving consistent results. This kit is compatible with many downstream applications of RNA like RT-qPCR, RNA-seq, or cDNA synthesis.

Example protocol

AT A GLANCE

Protocol summary

Add Helixyte™ Red RNA HS reagent working solution (200 µL).
Add test samples (10 µL).
Incubate at room temperature for 5 minutes.
Monitor fluorescence intensity at Ex/Em = 643/675 nm.

Important: The following protocol is provided as an example for quantifying total RNA with Helixyte™ Red RNA HS. Warm all the components to room temperature before opening. No data are available for the mutagenicity or toxicity of Helixyte™ Red RNA HS stain. Because this reagent binds to nucleic acids, it should be treated as a potential mutagen and handled with appropriate care. The DMSO stock solution should be handled with particular caution as DMSO is known to facilitate the entry of organic molecules into tissues.

PREPARATION OF STANDARD SOLUTIONS

For convenience, use the Serial Dilution Planner:
https://www.aatbio.com/tools/serial-dilution/17696

RNA Standard

Dilute 2 mg/mL RNA standard (Component C) to 10, 8, 6, 4, 2, 1, 0.5 ng/uL in RNA Assay Buffer (Component B).

PREPARATION OF WORKING SOLUTION

Add 2.5 μL Helixyte™ Red RNA HS (Component A) into 1 mL of RNA Assay Buffer (Component B) and mix well. Protect the working solution from light by covering it with foil or placing it in the dark.

Note: 1 mL of working solution is enough for 5 tests.

Note: We recommend preparing this solution in a plastic container rather than glass, as the dye may adsorb to glass surfaces. For best results, this solution should be used within a few hours of its preparation.

SAMPLE EXPERIMENTAL PROTOCOL

Table 1: Layout of RNA standards and test samples in a solid black 96-well microplate. RS=RNA Standards (RS1-RS7, 100 to 5 ng/well); BL=Blank Control; TS=Test Samples

BL	BL	TS	TS
RS1	RS1	TS	TS
RS2	RS2	TS	TS
RS3	RS3
RS4	RS4
RS5	RS5
RS6	RS6
RS7	RS7

Table 2: Reagent composition for each well.

Well	Volume	Reagent
RS1-RS7	10 µL	Serial Dilutions (100 to 5 ng/well)
BL	10 µL	Assay buffer
TS	10 µL	Test Sample

Add 200 μL of dye working solution to each well of RNA Standard, blank control, and test samples. For a 384-wellplate, add 100 μL of dye working solution into each well instead.
Prepare RNA Standards (RS), blank controls (BL), and test samples (TS) according to the layout provided in Tables 1 and 2. For a 384-well plate, use 5 μL of RNA standards or test samples per well instead of 10 μL.
Incubate the reaction at room temperature for 5 minutes, protected from light.
Monitor the fluorescence intensity with a fluorescence plate reader at Ex/Em = 640/675 nm (cut off at 665 nm).

References

View all 50 references: Citation Explorer

Universal nucleic acid donor screening revealed epidemiological features of hepatitis E and prevented transfusion-transmitted infection in Japan.
Authors: Tanaka, Ami and Matsubayashi, Keiji and Odajima, Takeshi and Sakata, Hidekatsu and Iida, Juri and Kai, Kazuhiro and Goto, Naoko and Satake, Masahiro
Journal: Transfusion (2024): 335-347

Preservation of functionality, immunophenotype, and recovery of HIV RNA from PBMCs cryopreserved for more than 20 years.
Authors: Dyer, Wayne B and Suzuki, Kazuo and Levert, Angelique and Starr, Mitchell and Lloyd, Andrew R and Zaunders, John J
Journal: Frontiers in immunology (2024): 1382711

Digital reverse transcription PCR using a simple poly(dimethylsiloxane) microwell array chip for detection of SARS-CoV-2.
Authors: Hosokawa, Kazuo and Ohmori, Hitoshi
Journal: Biochemical and biophysical research communications (2024): 151070

Context-dependent accuracy of the cobas plasma separation card for HCV RNA viral load measurement.
Authors: Qureshi, Huma and Duran, Ana Carrasco and Mahmood, Hassan and Sarwar, Zahida and Mahmood, Khalid and Midde, Krishna and Canchola, Jesse A and Parkin, Neil T and La Brot, Benjamin
Journal: Journal of viral hepatitis (2024): 156-160

Highly multiplexed mRNA quantitation with CRISPR-Cas13.
Authors: Kang, Brian and Zhang, Jiayu and Schwoerer, Michael P and Nelson, Amy N and Schoeman, Emily and Guo, Andrew and Ploss, Alexander and Myhrvold, Cameron
Journal: bioRxiv : the preprint server for biology (2023)

Page updated on April 25, 2025

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