Name: Helixyte™ Fluorimetric RNA Quantification Kit *5-100 ng High Sensitivity*
Brand: AAT Bioquest
SKU: 17696
Price: 301 USD
Availability: InStock

Helixyte™ Fluorimetric RNA Quantification Kit

5-100 ng High Sensitivity

Helixyte™ Fluorimetric RNA Quantification Kit enables sensitive and specific RNA measurement at low concentrations for advanced molecular biology workflows.

Ultra-sensitive detection: Accurately quantifies RNA in the 5–100 ng range using a fluorescence-based assay
High specificity: Selectively binds RNA with minimal interference from DNA, proteins, or free nucleotides
Applications: Suitable for RT-qPCR, RNA-seq, cDNA synthesis, and low-input RNA studies
Comparable alternative: Comparable to Qubit RNA High Sensitivity Assay Kits while enabling use with standard fluorescence microplate readers

RNA dose response was measured with Helixyte™ Fluorimetric RNA Quantitation Assay Kit *High Sensitivity* in a 96-well solid black plate.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17696	500 Test	Price

Overview

The Helixyte™ Fluorimetric RNA Quantification Kit (High Sensitivity) is designed for accurate and ultra-sensitive measurement of RNA at very low concentrations. Ideal for precious or limited RNA samples, this kit uses an advanced RNA-selective fluorescent dye to achieve high specificity and minimal background noise, offering a clear advantage over traditional UV absorbance methods.Helixyte™ Fluorimetric RNA Quantification Kit is a high-capacity format providing reagents sufficient for 500 tests. It provides reliable quantification across a variety of sample types like total RNA, mRNA, or small RNA fragments. Its streamlined protocol and ready-to-use format make it easy to integrate into existing workflows, saving time and giving consistent results. This kit is compatible with many downstream applications of RNA like RT-qPCR, RNA-seq, or cDNA synthesis.

References

View all 50 references: Citation Explorer

Universal nucleic acid donor screening revealed epidemiological features of hepatitis E and prevented transfusion-transmitted infection in Japan.

Authors:

Tanaka, Ami and Matsubayashi, Keiji and Odajima, Takeshi and Sakata, Hidekatsu and Iida, Juri and Kai, Kazuhiro and Goto, Naoko and Satake, Masahiro

Journal:

Transfusion (2024): 335-347

Preservation of functionality, immunophenotype, and recovery of HIV RNA from PBMCs cryopreserved for more than 20 years.

Authors:

Dyer, Wayne B and Suzuki, Kazuo and Levert, Angelique and Starr, Mitchell and Lloyd, Andrew R and Zaunders, John J

Journal:

Frontiers in immunology (2024): 1382711

Digital reverse transcription PCR using a simple poly(dimethylsiloxane) microwell array chip for detection of SARS-CoV-2.

Authors:

Hosokawa, Kazuo and Ohmori, Hitoshi

Journal:

Biochemical and biophysical research communications (2024): 151070

Context-dependent accuracy of the cobas plasma separation card for HCV RNA viral load measurement.

Authors:

Qureshi, Huma and Duran, Ana Carrasco and Mahmood, Hassan and Sarwar, Zahida and Mahmood, Khalid and Midde, Krishna and Canchola, Jesse A and Parkin, Neil T and La Brot, Benjamin

Journal:

Journal of viral hepatitis (2024): 156-160

Highly multiplexed mRNA quantitation with CRISPR-Cas13.

Authors:

Kang, Brian and Zhang, Jiayu and Schwoerer, Michael P and Nelson, Amy N and Schoeman, Emily and Guo, Andrew and Ploss, Alexander and Myhrvold, Cameron

Journal:

bioRxiv : the preprint server for biology (2023)

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	640 nm
Emission	675 nm
Cutoff	665 nm
Recommended plate	Solid black

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Page updated on August 30, 2026

Component A: Helixyte™ Red RNA HS	1 vial (0.25 mL,100X in DMSO)
Component B: RNA Assay Buffer	1 bottle (100 mL)
Component C: RNA Standard	1 vial (RNA: 2 mg/mL)