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Helixyte™ Green
10,000X Aqueous PCR Solution
Helixyte™ Green is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Green (SYBR® is the trademark of ThermoFisher). Helixyte™ Green is an excellent nucleic acid sensor. The dye is essentially nonfluorescent, but becomes highly fluorescent upon binding to dsDNA. Helixyte™ Green is much less mutagenic and toxic than ethidium bromide. It has the same spectral properties to those of PicoGreen®, thus a great replacement to PicoGreen® (PicoGreen® is the trademark of Invitrogen). Helixyte™ Green is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA). It has much greater sensitivity for dsDNA, thus especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. Helixyte™ Green dye has excitation and emission spectra identical to that of PicoGreen®, making the dye readily compatible with instrument settings of PicoGreen® or SYBR® Green. This particular Helixyte™ Green formulation is optimized for quantitative real-time PCR (qPCR) applications.
Quantitative PCR results targeting GAPDH with an input of 100 ng–0.00001 ng cDNA was performed using Helixyte™ Green *10,000X Aqueous PCR Solution* (Cat No. 17592) and a Fast Advanced Master Mix on an Applied Biosystems® 7500 FAST Real-Time PCR System.
Quantitative PCR results targeting GAPDH with an input of 100 ng–0.00001 ng cDNA was performed using Helixyte™ Green *10,000X Aqueous PCR Solution* (Cat No. 17592) and a Fast Advanced Master Mix on an Applied Biosystems® 7500 FAST Real-Time PCR System.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
175921 ml
Price
 
Physical properties
Molecular weightN/A
SolventDMSO
Spectral properties
Excitation (nm)498
Emission (nm)522
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on February 12, 2025