Helixyte Green (SYBR Green I)

Helixyte™ Green

10,000X Aqueous PCR Solution

Helixyte™ Green is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Green (SYBR® is the trademark of ThermoFisher). Helixyte™ Green is an excellent nucleic acid sensor. The dye is essentially nonfluorescent, but becomes highly fluorescent upon binding to dsDNA. Helixyte™ Green is much less mutagenic and toxic than ethidium bromide. It has the same spectral properties to those of PicoGreen®, thus a great replacement to PicoGreen® (PicoGreen® is the trademark of Invitrogen). Helixyte™ Green is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA). It has much greater sensitivity for dsDNA, thus especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. Helixyte™ Green dye has excitation and emission spectra identical to that of PicoGreen®, making the dye readily compatible with instrument settings of PicoGreen® or SYBR® Green. This particular Helixyte™ Green formulation is optimized for quantitative real-time PCR (qPCR) applications.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17592	1 ml	Price

References

View all 22 references: Citation Explorer

Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice

Authors:

Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.

Journal:

Stroke (2012): 524

Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence

Authors:

Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.

Journal:

Lasers Surg Med (2010): 630

Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques

Authors:

van 't Hoff M, Reuter M, Dryden DT, Oheim M.

Journal:

Phys Chem Chem Phys (2009): 7713

Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians

Authors:

Lee BC, Kim S, Soh KS.

Journal:

J Acupunct Meridian Stud (2008): 29

Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy

Authors:

Shimizu M, Sasaki S, Kinjo M.

Journal:

Anal Biochem (2007): 87

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Excitation (nm)	498
Emission (nm)	522

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

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Page updated on February 12, 2025