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Helixyte™ Green *10,000X Aqueous PCR Solution*

Quantitative PCR results targeting GAPDH with an input of 100 ng–0.00001 ng cDNA was performed using Helixyte™ Green *10,000X Aqueous PCR Solution* (Cat No. 17592) and a Fast Advanced Master Mix on an Applied Biosystems® 7500 FAST Real-Time PCR System.
Quantitative PCR results targeting GAPDH with an input of 100 ng–0.00001 ng cDNA was performed using Helixyte™ Green *10,000X Aqueous PCR Solution* (Cat No. 17592) and a Fast Advanced Master Mix on an Applied Biosystems® 7500 FAST Real-Time PCR System.
Ordering information
Price ()
Catalog Number17592
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weightN/A
SolventDMSO
Spectral properties
Excitation (nm)498
Emission (nm)522
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


Molecular weight
N/A
Excitation (nm)
498
Emission (nm)
522
Helixyte™ Green is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Green (SYBR® is the trademark of ThermoFisher). Helixyte™ Green is an excellent nucleic acid sensor. The dye is essentially nonfluorescent, but becomes highly fluorescent upon binding to dsDNA. Helixyte™ Green is much less mutagenic and toxic than ethidium bromide. It has the same spectral properties to those of PicoGreen®, thus a great replacement to PicoGreen® (PicoGreen® is the trademark of Invitrogen). Helixyte™ Green is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA). It has much greater sensitivity for dsDNA, thus especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. Helixyte™ Green dye has excitation and emission spectra identical to that of PicoGreen®, making the dye readily compatible with instrument settings of PicoGreen® or SYBR® Green. This particular Helixyte™ Green formulation is optimized for quantitative real-time PCR (qPCR) applications.

Spectrum


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spectrum

Spectral properties

Excitation (nm)498
Emission (nm)522

References


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Journal: Biomacromolecules (2005): 2703
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Journal: Cell Oncol (2005): 225
TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual-colour FISH on paraffin sections
Authors: Bink K, Walch A, Feuchtinger A, Eisenmann H, Hutzler P, Hofler H, Werner M.
Journal: Histochem Cell Biol (2001): 293
Oxazole yellow homodimer YOYO-1-labeled DNA: a fluorescent complex that can be used to assess structural changes in DNA following formation and cellular delivery of cationic lipid DNA complexes
Authors: Wong M, Kong S, Dragowska WH, Bally MB.
Journal: Biochim Biophys Acta (2001): 61
Photophysical properties of fluorescent DNA-dyes bound to single- and double-stranded DNA in aqueous buffered solution
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Journal: Photochem Photobiol (2001): 585