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AAT Bioquest

Helixyte™ Green Alkyne

Helixyte™ Green is an excellent nucleic acid sensor that exhibits large fluorescence enhancement upon binding to dsDNA. It has the same spectral and DNA-binding properties as SYBR Green® (SYBR Green® is the trademark of ThermoFisher). AAT Bioquest’s scientists developed Helixyte™ Green alkyne to enable the development of site-specific DNA probes. Helixyte™ Green alkyne is an excellent building block that readily reacts with azido-containing biological molecules such as azido-modified peptides, antibodies and oligos. Thus, it can be used to develop a variety of site-directed DNA probes. Helixyte™ Green is an excellent fluorogenic probe for quantifying DNAs with greatly improved sensitivity and selectivity. Helixyte™ Green is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biological procedures such as cDNA synthesis for library production and DNA fragment purification for subcloning, as well as diagnostic applications, such as quantitating DNA amplification products and primer extension assays.

Spectrum

References

View all 50 references: Citation Explorer
A method for selective and efficient isolation of gray matter astrocytes from the spinal cord of adult mice.
Authors: Iwasaki, Ryoma and Kohro, Yuta and Tsuda, Makoto
Journal: Molecular brain (2024): 25
A multi-biomarker micronucleus assay using imaging flow cytometry.
Authors: Harte, Danielle S G and Lynch, Anthony M and Verma, Jatin and Rees, Paul and Filby, Andrew and Wills, John W and Johnson, George E
Journal: Archives of toxicology (2024): 3137-3153
Clickable APEX2 Probes for Enhanced RNA Proximity Labeling in Live Cells.
Authors: Liang, Jiying and Han, Jinghua and Gao, Xutao and Jia, Han and Li, Ran and Tse, Edmund C M and Li, Ying
Journal: Analytical chemistry (2024): 685-693
Purification of Planarian Stem Cells Using a Draq5-Based FACS Approach.
Authors: Wang, Kuang-Tse and Tapper, Justin and Adler, Carolyn E
Journal: Methods in molecular biology (Clifton, N.J.) (2024): 203-212
A flow cytometry method for safe detection of bacterial viability.
Authors: Servain-Viel, S and Aknin, M-L and Domenichini, S and Perlemuter, G and Cassard, A-M and Schlecht-Louf, G and Moal, V Lievin-Le
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2024): 146-156
Page updated on May 19, 2025

Ordering information

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Unit size
Catalog Number17615
Quantity
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Additional ordering information

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Physical properties

Molecular weight

1001.00

Solvent

Water

Spectral properties

Excitation (nm)

498

Emission (nm)

522

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure