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Helixyte™ Green Azide

Helixyte™ Green is an excellent nucleic acid sensor that exhibits large fluorescence enhancement upon binding to dsDNA. It has the same spectral and DNA-binding properties as SYBR Green® (SYBR Green® is the trademark of ThermoFisher). AAT Bioquest’s scientists developed Helixyte™ Green azide to enable the development of site-specific DNA probes. Helixyte™ Green azide is an excellent building block that readily reacts with alkyne-containing biological molecules such as alkyne-modified peptides, antibodies and oligos. It also readily reacts with DBCO-modified biomolecules. Thus, it can be used to develop a variety of site-directed DNA probes. Helixyte™ Green is an excellent fluorogenic probe for quantifying DNAs with greatly improved sensitivity and selectivity. Helixyte™ Green is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biological procedures such as cDNA synthesis for library production and DNA fragment purification for subcloning, as well as diagnostic applications, such as quantitating DNA amplification products and primer extension assays.

Spectrum

References

View all 50 references: Citation Explorer
In Migratio Noncovalent Fluorophore Labeling of Proteins by Propidium Iodide in Sodium Dodecyl Sulfate Capillary Gel Electrophoresis.
Authors: Auer, Felicia and Guttman, Andras
Journal: Analytical chemistry (2024): 10969-10977
Rapid determination of antibiotic susceptibility of clinical isolates of Escherichia coli by SYBR green I/Propidium iodide assay.
Authors: Cui, Xianglun and Liu, Shuyue and Jin, Yan and Li, Mingyu and Shao, Chunhong and Yu, Hong and Zhang, Ying and Liu, Yun and Wang, Yong
Journal: Scientific reports (2024): 18782
Point-of-Care Diagnostic System for Viable Salmonella Species via Improved Propidium Monoazide and Recombinase Polymerase Amplification Based Nucleic Acid Lateral Flow.
Authors: Lee, So-Young and Oh, Se-Wook
Journal: Diagnostics (Basel, Switzerland) (2024)
Selective Elucidation of Living Microbial Communities in Fermented Grains of Chinese Baijiu: Development of a Technique Integrating Propidium Monoazide Probe Pretreatment and Amplicon Sequencing.
Authors: Bo, Tao and Zhang, Jiaojiao and Zong, Enxiang and Lv, Na and Bai, Baoqing and Yang, Yukun and Zhang, Jinhua and Fan, Sanhong
Journal: Foods (Basel, Switzerland) (2024)
Development of a Long-Amplicon Propidium Monoazide-Quantitative PCR Assay for Detection of Viable Xanthomonas arboricola pv. pruni Cells in Peach Trees.
Authors: Panth, Milan and Noh, Enoch and Schnabel, Guido and Wang, Hehe
Journal: Plant disease (2024): PDIS01240012RE
Page updated on May 19, 2025

Ordering information

Price
Unit size
Catalog Number17616
Quantity
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Additional ordering information

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Physical properties

Molecular weight

1032.02

Solvent

Water

Spectral properties

Excitation (nm)

498

Emission (nm)

522

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure