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Helixyte™ Green Fluorimetric dsDNA Quantitation Kit
High Sensitivity
Helixyte™ Green dsDNA Quantitation Assay Kit can be used for selectively detecting as little as 25 pg/ml of dsDNA in the presence of ssDNA, RNA, and free nucleotides. Helixyte™ Green exhibits large fluorescence enhancement upon binding to dsDNA. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA, miniprep DNA, or PCR amplification products. Helixyte™ Green dsDNA Quantitation Assay Kit is a few magnitudes more sensitive than UV absorbance readings. It is specific for dsDNA in the presence of equimolar amounts of RNA. The kit is robust with a mix and read format. It can be used with a bench top fluorometer or a hand-held fluorescence meter (e.g., Qubit fluorometer). This kit is an excellent replacement for Quant-iT™ PicoGreen® dsDNA Assay Kit (Quant-iT™ and PicoGreen® are the trademarks of Invitrogen).
Comparison of dsDNA dose response using the Helixyte Green&trade; (blue) with Invitrogen<sup>TM</sup> Quant-iT&trade; PicoGreen&reg; dsDNA Reagent (red ). dsNDA standards were incubated in cuvettes and measured using varian cary eclipse fluorescence spectrophotometer.&nbsp;
Comparison of dsDNA dose response using the Helixyte Green&trade; (blue) with Invitrogen<sup>TM</sup> Quant-iT&trade; PicoGreen&reg; dsDNA Reagent (red ). dsNDA standards were incubated in cuvettes and measured using varian cary eclipse fluorescence spectrophotometer.&nbsp;
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
176511000 Tests
Price
 
Spectral properties
Excitation (nm)502
Emission (nm)522
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
UNSPSC41116134
Instrument settings
Spectrofluorometer
Excitation490 nm
Emission525 nm
Cutoff515 nm
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Page updated on October 1, 2025