Name: Helixyte™ iFluor® 488 Nucleic Acid Labeling Dye *Optimized for Labeling 100-300 ug DNA/RNA*
Brand: AAT Bioquest
SKU: 17955
Price: 516 USD
Availability: InStock

Helixyte™ iFluor® 488 Nucleic Acid Labeling Dye

Optimized for Labeling 100-300 ug DNA/RNA

Helixyte™ iFluor® 488 Nucleic Acid Labeling Dye enables rapid, covalent fluorescent labeling of DNA and RNA for nucleic acid detection and hybridization applications.

Nucleic Acid Labeling: Forms stable covalent bonds with the N7 position of guanine.
Spectral Properties: Excitation at 491 nm and emission at 516 nm.
Simple Workflow: Rapid mixing reaction with high labeling yields.
Fluorescence: Bright, stable fluorescence detectable with FITC filter sets.
Applications: Suitable for Northern/Southern blotting, FISH, CGH, and microarray analysis.

Direct labeling of nucleic acid using Helixyte™ iFluor® 488 Nucleic Acid Labeling Dye. The DNA ladder was labeled with 100 µM of Helixyte™ iFluor® 488 Nucleic Acid Labeling Dye (Lane 7), with ThermoFisher's Ulysis™ Alexa Fluor™ 488 Nucleic acid labeling kit (Lane 1) and analyzed alongside unlabeled DNA (Lane 2) on 1% agarose DNA gel using gel electrophoresis.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17955	1 mg	Price

Overview

Helixyte™ iFluor® 488 Nucleic Acid Labeling Dye is a key member of our enabling Helixyte™ nucleic acid labeling and conjugation technology. The labeling/conjugation of a tag/hapten to nucleic acids has been very challenging due to the lack of reactive moieties in nucleic acid molecules. Thymine and guanosine have been often explored for nucleic acid conjugations, e.g., photo-crosslink (to thymine by psoralens) or bromination/Ulysis labeling of guanosine. However, these existing conjugations are either tedious or require stringent conditions with low yields and are thus not suitable for routine lab use. Under the similar conditions, our Helixyte™ nucleic acid labeling and conjugation technology is much easier to use with significantly higher yield. Helixyte™ iFluor® 488 Nucleic Acid Labeling Dye provides a unique method to attach the iFluor® 488 fluorophore to nucleic acids via a simple mixing step. The labeling reagent readily reacts with the N7 of guanine to form a stable covalent bond. The labeling procedure is simple and fast with a high production yield. The separation of the labeled nucleic acids from the unreacted dye can be accomplished with a simple ethanol precipitation, a spin-column or dialysis. The resulting labeled DNA/RNA probes have bright green and stable fluorescence that can be easily detected with FITC filter set. They can be used for dot, Northern and Southern blots, RNA and DNA in situ hybridization, multicolor fluorescence in situ hybridization (mFISH), comparative genome hybridization (CGH) or microarray analysis etc.

References

View all 50 references: Citation Explorer

Proximity Estimation and Quantification of Ionizing Radiation-induced DNA Lesions in Aqueous Media using Fluorescence Spectroscopy.

Authors:

Akamatsu, Ken and Satoh, Katsuya and Shikazono, Naoya and Saito, Takeshi

Journal:

Radiation research (2023)

Possible frequent multiple mitochondrial DNA copies in a single nucleoid in HeLa cells.

Authors:

Pavluch, Vojtěch and Špaček, Tomáš and Engstová, Hana and Dlasková, Andrea and Ježek, Petr

Journal:

Scientific reports (2023): 5788

Exclusion of Anchor-Matched Peptide Nucleic Acid from Liquid-Ordered Domains by Hybridization with Complementary Flavin-Labeled DNA.

Authors:

Oka, Yoshimi

Journal:

ACS omega (2023): 1109-1113

1,4-Naphthoquinone (CNN1) Induces Apoptosis through DNA Damage and Promotes Upregulation of H2AFX in Leukemia Multidrug Resistant Cell Line.

Authors:

de Sousa Portilho, Adrhyann Jullyanne and da Silva, Emerson Lucena and Bezerra, Emanuel Cintra Austregésilo and Moraes Rego Gomes, Carinne Borges de Souza and Ferreira, Vitor and de Moraes, Maria Elisabete Amaral and da Rocha, David Rodrigues and Burbano, Rommel Mário Rodriguez and Moreira-Nunes, Caroline Aquino and Montenegro, Raquel Carvalho

Journal:

International journal of molecular sciences (2022)

Capturing a Pentacyclic Fragment-Based Library Derived from Perophoramidine: Their Design, Synthesis and Evaluation as Anticancer Compounds by DNA Double-Strand Breaks (DSB) and PARP-1 Inhibition.

Authors:

Guha, Souvik and Yussif El-Deeb, Ibrahim and Yadav, Shalini and Das, Ranajit and Dutta Dubey, Kshatresh and Baruah, Mousumi and Gremaud, Ludovic and Sen, Subhabrata

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2022): e202202405

Physical properties

Solvent

DMSO

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Page updated on October 25, 2026