Name: Helixyte™ iFluor® 555 Nucleic Acid Labeling Dye *Optimized for Labeling 100-300 ug DNA/RNA*
Brand: AAT Bioquest
SKU: 17958
Price: 516 USD
Availability: InStock

Helixyte™ iFluor® 555 Nucleic Acid Labeling Dye

Optimized for Labeling 100-300 ug DNA/RNA

Helixyte™ iFluor® 555 Nucleic Acid Labeling Dye enables rapid, covalent fluorescent labeling of DNA and RNA for nucleic acid detection and hybridization applications.

Nucleic Acid Labeling: Forms stable covalent bonds with the N7 position of guanine.
Spectral Properties: Excitation at 557 nm and emission at 570 nm.
Simple Workflow: Rapid mixing reaction with high labeling yields.
Fluorescence: Bright, stable fluorescence detectable with Cy3 filter sets.
Applications: Suitable for Northern/Southern blotting, FISH, CGH, and microarray analysis.

Direct labeling of nucleic acid using Helixyte™ iFluor® 555 Nucleic Acid Labeling Dye. DNA ladder was labeled with 50 µM of Helixyte™ iFluor® 555 Nucleic Acid Labeling Dye (Lane 4) and analyzed alongside unlabeled DNA (Lane 1) on 1% agarose DNA gel using gel electrophoresis.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17958	1 mg	Price

Overview

Helixyte™ iFluor® 555 Nucleic Acid Labeling Dye is a key member of our enabling Helixyte™ nucleic acid labeling and conjugation technology. The labeling/conjugation of a tag/hapten to nucleic acids has been very challenging due to the lack of reactive moieties in nucleic acid molecules. Thymine and guanosine have been often explored for nucleic acid conjugations, e.g., photo-crosslink (to thymine by psoralens) or bromination/Ulysis labeling of guanosine. However, these existing conjugation techniques are either tedious, ineffective or require stringent conditions with low yields and are thus not suitable for routine lab use. Under the similar conditions, our Helixyte™ nucleic acid labeling and conjugation technology is much easier to use with significantly higher yield. Helixyte™ iFluor® 555 Nucleic Acid Labeling Dye provides a unique method to attach the iFluor® 555 fluorophore to nucleic acids via a simple mixing step. The labeling reagent readily reacts with the N7 of guanine to form a stable covalent bond. The labeling procedure is simple and fast with a high production yield. The separation of the labeled nucleic acids from the unreacted dye can be accomplished with a simple ethanol precipitation, a spin-column or dialysis. The resulting labeled DNA/RNA probes have bright red and stable fluorescence that can be easily detected with Cy3 filter set. They can be used for dot, Northern and Southern blots, RNA and DNA in situ hybridization, multicolor fluorescence in situ hybridization (mFISH), comparative genome hybridization (CGH) or microarray analysis etc.

References

View all 6 references: Citation Explorer

A novel pH/light-triggered surface for DNA adsorption and release.

Authors:

Birlik Demirel, Gökçen

Journal:

Chemphyschem : a European journal of chemical physics and physical chemistry (2014): 1693-9

Probing quenched dye fluorescence of Cy3-DNA-Au-nanoparticle hybrid conjugates using solution and array platforms.

Authors:

Obliosca, Judy M and Wang, Pen-Cheng and Tseng, Fan-Gang

Journal:

Journal of colloid and interface science (2012): 34-41

High-resolution epifluorescence and time-of-flight secondary ion mass spectrometry chemical imaging comparisons of single DNA microarray spots.

Authors:

Rao, Archana N and Vandencasteele, Nicolas and Gamble, Lara J and Grainger, David W

Journal:

Analytical chemistry (2012): 10628-36

Label-free detection of DNA-binding proteins based on microfluidic solid-state molecular beacon sensor.

Authors:

Wang, Jun and Onoshima, Daisuke and Aki, Michihiko and Okamoto, Yukihiro and Kaji, Noritada and Tokeshi, Manabu and Baba, Yoshinobu

Journal:

Analytical chemistry (2011): 3528-32

Rapid transport of plasmid DNA into the nucleolus via actin depolymerization using the HVJ envelope vector.

Authors:

Suvanasuthi, Saroj and Tamai, Katsuto and Kaneda, Yasufumi

Journal:

The journal of gene medicine (2007): 55-62

Physical properties

Solvent

DMSO

Spectral properties

Correction factor (260 nm)	0.23
Correction factor (280 nm)	0.14
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.64 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Email	sales@aatbio.com
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Page updated on October 25, 2026