Helixyte™ iFluor® 594 Nucleic Acid Labeling Dye *Optimized for Labeling 100-300 ug DNA/RNA*

Helixyte™ iFluor® 594 Nucleic Acid Labeling Dye

Optimized for Labeling 100-300 ug DNA/RNA

Helixyte™ iFluor® 594 Nucleic Acid Labeling Dye enables rapid, covalent fluorescent labeling of DNA and RNA for nucleic acid detection and hybridization applications.

Nucleic Acid Labeling: Forms stable covalent bonds with the N7 position of guanine.
Spectral Properties: Excitation at 587 nm and emission at 603 nm.
Simple Workflow: Rapid mixing reaction with high labeling yields.
Fluorescence: Bright, stable fluorescence detectable with Texas Red filter sets.
Applications: Suitable for Northern/Southern blotting, FISH, CGH, and microarray analysis.

Direct labeling of nucleic acid using Helixyte™ iFluor® 594 Nucleic Acid Labeling Dye. DNA ladder was labeled with 50 µM of Helixyte™ iFluor® 594 Nucleic Acid Labeling Dye (Lane 3) and analyzed alongside unlabeled DNA (Lane 2) on 1% agarose DNA gel using gel electrophoresis.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17960	1 mg	Price

Overview

Helixyte™ iFluor® 594 Nucleic Acid Labeling Dye is a key member of our enabling Helixyte™ nucleic acid labeling and conjugation technology. The labeling/conjugation of a tag/hapten to nucleic acids has been very challenging due to the lack of reactive moieties in nucleic acid molecules. Thymine and guanosine have been often explored for nucleic acid conjugations, e.g., photo-crosslink (to thymine by psoralens) or bromination/Ulysis labeling of guanosine. However, these existing conjugation techniques are either tedious, ineffective or require stringent conditions with low yields and are thus not suitable for routine lab use. Under the similar conditions, our Helixyte™ nucleic acid labeling and conjugation technology is much easier to use with significantly higher yield. Helixyte™ iFluor® 594 Nucleic Acid Labeling Dye provides a unique method to attach the iFluor® 594 fluorophore to nucleic acids via a simple mixing step. The labeling reagent readily reacts with the N7 of guanine to form a stable covalent bond. The labeling procedure is simple and fast with a high production yield. The separation of the labeled nucleic acids from the unreacted dye can be accomplished with a simple ethanol precipitation, a spin-column or dialysis. The resulting labeled DNA/RNA probes have bright red and stable fluorescence that can be easily detected with Texas Red filter set. They can be used for dot, Northern and Southern blots, RNA and DNA in situ hybridization, multicolor fluorescence in situ hybridization (mFISH), comparative genome hybridization (CGH) or microarray analysis etc.

References

View all 50 references: Citation Explorer

Label-free virtual staining of neutrophil extracellular traps (NETs) in microfluidics.

Authors:

Petchakup, Chayakorn and Wong, Siong Onn and Dalan, Rinkoo and Hou, Han Wei

Journal:

Lab on a chip (2023): 3936-3944

Alkaline Comet Assay to Detect DNA Damage.

Authors:

Walsh, Kade D and Kato, Takamitsu A

Journal:

Methods in molecular biology (Clifton, N.J.) (2023): 65-72

Synthesis of Substituted Cy5 Phosphoramidite Derivatives and Their Incorporation into Oligonucleotides Using Automated DNA Synthesis.

Authors:

Meares, Adam and Susumu, Kimihiro and Mathur, Divita and Lee, Sang Ho and Mass, Olga A and Lee, Jeunghoon and Pensack, Ryan D and Yurke, Bernard and Knowlton, William B and Melinger, Joseph S and Medintz, Igor L

Journal:

ACS omega (2022): 11002-11016

Photocaged Hoechst Enables Subnuclear Visualization and Cell Selective Staining of DNA in vivo.

Authors:

Lämmle, Carina A and Varady, Adam and Müller, Thorsten G and Sturtzel, Caterina and Riepl, Michael and Mathes, Bettina and Eichhorst, Jenny and Sporbert, Anje and Lehmann, Martin and Kräusslich, Hans-Georg and Distel, Martin and Broichhagen, Johannes

Journal:

Chembiochem : a European journal of chemical biology (2021): 548-556

A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice.

Authors:

Simonetti, Sonia and Natalini, Ambra and Peruzzi, Giovanna and Nicosia, Alfredo and Folgori, Antonella and Capone, Stefania and Santoni, Angela and Di Rosa, Francesca

Journal:

Journal of visualized experiments : JoVE (2021)

Physical properties

Solvent

DMSO

Spectral properties

Absorbance (nm)	587
Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	200000 ¹
Excitation (nm)	587
Emission (nm)	603
Quantum yield	0.53 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 25, 2026