Helixyte™ iFluor® 647 Nucleic Acid Labeling Dye *Optimized for Labeling 100-300 ug DNA/RNA*

Helixyte™ iFluor® 647 Nucleic Acid Labeling Dye

Optimized for Labeling 100-300 ug DNA/RNA

Helixyte™ iFluor® 647 Nucleic Acid Labeling Dye enables rapid, covalent fluorescent labeling of DNA and RNA for nucleic acid detection and hybridization applications.

Nucleic Acid Labeling: Forms stable covalent bonds with the N7 position of guanine.
Spectral Properties: Excitation at 655 nm and emission at 670 nm.
Simple Workflow: Rapid mixing reaction with high labeling yields.
Fluorescence: Bright, stable fluorescence detectable with Cy5 filter sets.
Applications: Suitable for Northern/Southern blotting, FISH, CGH, and microarray analysis.

<b>Gel migration and cellular uptake of Helixyte™ iFluor® 647-labeled siRNA.</b>
(A) Gel pattern for 100 ng each of unlabeled siRNA, 100 µM Helixyte™ iFluor® 647-siRNA, and 200 µM Helixyte™ iFluor® 647-siRNA on a 2% agarose gel stained with CyberOrange. Unlabeled and labeled siRNAs showed similar migration patterns. Notably, labeled siRNAs appeared as red bands under a Cy5 filter, while unlabeled siRNA was visible as blue under a Sypro Ruby filter.
(B) Fluorescence microscopy of HeLa cells transfected with 50 nM Helixyte™ iFluor® 647-labeled siRNA using Transfectamine 7000. Observations at 24, 48, and 72 hours post-transfection under a Cy5 filter demonstrated successful siRNA uptake detected as early as 24 hours, with fluorescence intensity increasing over time, indicating progressive cellular incorporation of labeled siRNA.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17963	1 mg	Price

Overview

Helixyte™ iFluor® 647 Nucleic Acid Labeling Dye is a key member of our enabling Helixyte™ nucleic acid labeling and conjugation technology. The labeling/conjugation of a tag/hapten to nucleic acids has been very challenging due to the lack of reactive moieties in nucleic acid molecules. Thymine and guanosine have been often explored for nucleic acid conjugations, e.g., photo-crosslink (to thymine by psoralens) or bromination/Ulysis labeling of guanosine. However, these existing conjugation techniques are either tedious, ineffective or require stringent conditions with low yields and are thus not suitable for routine lab use. Under the similar conditions, our Helixyte™ nucleic acid labeling and conjugation technology is much easier to use with significantly higher yield. Helixyte™ iFluor® 647 Nucleic Acid Labeling Dye provides a unique method to attach the iFluor® 647 fluorophore to nucleic acids via a simple mixing step. The labeling reagent readily reacts with the N7 of guanine to form a stable covalent bond. The labeling procedure is simple and fast with a high production yield. The separation of the labeled nucleic acids from the unreacted dye can be accomplished with a simple ethanol precipitation, a spin-column or dialysis. The resulting labeled DNA/RNA probes have bright red and stable fluorescence that can be easily detected with Cy5 filter set. They can be used for dot, Northern and Southern blots, RNA and DNA in situ hybridization, multicolor fluorescence in situ hybridization (mFISH), comparative genome hybridization (CGH) or microarray analysis etc.

References

View all 38 references: Citation Explorer

Hydrophobicity Regulates the Cellular Interaction of Cyanine5-Labeled Poly(3-hydroxypropionate)-Based Comb Polymers.

Authors:

Mahmoud, Ayaat M and Nowell, Cameron J and Feeney, Orlagh and van 't Hag, Leonie and Davis, Thomas P and Kempe, Kristian

Journal:

Biomacromolecules (2022): 3560-3571

Bacteria-Mediated Intracellular Click Reaction for Drug Enrichment and Selective Apoptosis of Drug-Resistant Tumor Cells.

Authors:

Gao, Zhiqiang and Zhang, Endong and Zhao, Hao and Xia, Shengpeng and Bai, Haotian and Huang, Yiming and Lv, Fengting and Liu, Libing and Wang, Shu

Journal:

ACS applied materials & interfaces (2022): 12106-12115

A Dendron-Based Fluorescence Turn-On Probe for Tumor Detection.

Authors:

Liu, Changren and Zhang, Ling'e and Zhou, Sensen and Zhang, Xiaoke and Wu, Wei and Jiang, Xiqun

Journal:

Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 13022-13030

Determination of genetic aberrations and novel transcripts involved in the pathogenesis of oligodendroglioma using array comparative genomic hybridization and next generation sequencing.

Authors:

Hassanudin, Siti A and Ponnampalam, Stephen N and Amini, Muhammad N

Journal:

Oncology letters (2019): 1675-1687

Aptamer-Functionalized Activatable DNA Tetrahedron Nanoprobe for PIWI-Interacting RNA Imaging and Regulating in Cancer Cells.

Authors:

Jia, Ruichen and He, Xiaoxiao and Ma, Wenjie and Lei, Yanli and Cheng, Hong and Sun, Huanhuan and Huang, Jin and Wang, Kemin

Journal:

Analytical chemistry (2019): 15107-15113

Physical properties

Solvent

DMSO

Spectral properties

Correction factor (260 nm)	0.03
Correction factor (280 nm)	0.03
Correction factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	220000 ¹
Excitation (nm)	655
Emission (nm)	670
Quantum yield	0.25 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
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Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 25, 2026