AAT Bioquest

HEX-dT Phosphoramidite

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Physical properties
Molecular weight1632.23
Spectral properties
Correction Factor (280 nm)0.15
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)533
Emission (nm)559
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
HEX-dT phosphoramidite contains a DMT group to allow quantification of coupling. It can be inserted into a target sequence as a replacement for a dT residue. It provides a valuable tool to add the HEX tag to a desired location of an oligo sequence. It is complimentary to the commonly used 6-HEX phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. AAT Bioquest also offer fluorescein-dT phosphoramidite, TET-dT phosphoramidite, biotin-dT phosphoramidite, biotin-dT CPG and Fluorescein-dT CPG.


Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of HEX-dT Phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM61.266 µL306.329 µL612.659 µL3.063 mL6.127 mL
5 mM12.253 µL61.266 µL122.532 µL612.659 µL1.225 mL
10 mM6.127 µL30.633 µL61.266 µL306.329 µL612.659 µL

Molarity calculator

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Spectral properties

Correction Factor (280 nm)0.15
Extinction coefficient (cm -1 M -1)73000
Excitation (nm)533
Emission (nm)559



View all 5 references: Citation Explorer
Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes.
Authors: Skoblov, Aleksander Yu and Vichuzhanin, Maxim V and Farzan, Valentina M and Veselova, Olga A and Konovalova, Tatiana A and Podkolzin, Alexander T and Shipulin, German A and Zatsepin, Timofei S
Journal: Bioorganic & medicinal chemistry (2015): 6749-56
Acetylated and methylated β-cyclodextrins as viable soluble supports for the synthesis of short 2′-oligodeoxyribo-nucleotides in solution.
Authors: Molina, Alejandro Gimenez and Kungurtsev, Vyacheslav and Virta, Pasi and Lönnberg, Harri
Journal: Molecules (Basel, Switzerland) (2012): 12102-20
Sorting the unique chirality, right handed single wall carbon nanotubes via the dye modified ssDNA.
Authors: Liu, Ru and Chen, Zhong and Zhao, Feng and Chang, Xueling and Zu, Yan and Gao, Xueyun
Journal: Journal of nanoscience and nanotechnology (2011): 7587-92
A new synthetic protocol for labeled oligonucleotides, using a chemically cleavable universal linker.
Authors: Mahajan, Shweta and Patnaik, S and Kumar, P and Gandhi, R P and Gupta, K C
Journal: Bioorganic & medicinal chemistry (2006): 4302-9
Amplified fragment length polymorphism (AFLP) protocol for genotyping the malarial parasite Plasmodium falciparum.
Authors: Rubio, J M and Berzosa, P J and Benito, A
Journal: Parasitology (2001): 331-6