HEX-dT Phosphoramidite

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1632.23
Solvent	MeCN

Spectral properties

Correction Factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	533
Emission (nm)	559

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1632.23

Correction Factor (280 nm)

0.15

Extinction coefficient (cm ^-1 M ^-1)

73000

Excitation (nm)

533

Emission (nm)

559

HEX-dT phosphoramidite contains a DMT group to allow quantification of coupling. It can be inserted into a target sequence as a replacement for a dT residue. It provides a valuable tool to add the HEX tag to a desired location of an oligo sequence. It is complimentary to the commonly used 6-HEX phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. AAT Bioquest also offer fluorescein-dT phosphoramidite, TET-dT phosphoramidite, biotin-dT phosphoramidite, biotin-dT CPG and Fluorescein-dT CPG.

Calculators

Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of HEX-dT Phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	61.266 µL	306.329 µL	612.659 µL	3.063 mL	6.127 mL
5 mM	12.253 µL	61.266 µL	122.532 µL	612.659 µL	1.225 mL
10 mM	6.127 µL	30.633 µL	61.266 µL	306.329 µL	612.659 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	533
Emission (nm)	559

Images

Figure 1. Chemical structure for HEX-dT Phosphoramidite.

References

View all 5 references: Citation Explorer

Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes.
Authors: Skoblov, Aleksander Yu and Vichuzhanin, Maxim V and Farzan, Valentina M and Veselova, Olga A and Konovalova, Tatiana A and Podkolzin, Alexander T and Shipulin, German A and Zatsepin, Timofei S
Journal: Bioorganic & medicinal chemistry (2015): 6749-56

Acetylated and methylated β-cyclodextrins as viable soluble supports for the synthesis of short 2′-oligodeoxyribo-nucleotides in solution.
Authors: Molina, Alejandro Gimenez and Kungurtsev, Vyacheslav and Virta, Pasi and Lönnberg, Harri
Journal: Molecules (Basel, Switzerland) (2012): 12102-20

Sorting the unique chirality, right handed single wall carbon nanotubes via the dye modified ssDNA.
Authors: Liu, Ru and Chen, Zhong and Zhao, Feng and Chang, Xueling and Zu, Yan and Gao, Xueyun
Journal: Journal of nanoscience and nanotechnology (2011): 7587-92

A new synthetic protocol for labeled oligonucleotides, using a chemically cleavable universal linker.
Authors: Mahajan, Shweta and Patnaik, S and Kumar, P and Gandhi, R P and Gupta, K C
Journal: Bioorganic & medicinal chemistry (2006): 4302-9

Amplified fragment length polymorphism (AFLP) protocol for genotyping the malarial parasite Plasmodium falciparum.
Authors: Rubio, J M and Berzosa, P J and Benito, A
Journal: Parasitology (2001): 331-6