Hexidium Iodide *CAS 211566-66-4*

Hexidium Iodide

CAS 211566-66-4

The nucleic acid stain, hexidium iodide is permeant to mammalian cells and selectively stains almost all gram-positive bacteria in the presence of gram-negative bacteria. Hexidium iodide exhibits excitation/emission maxima of ~518/600 nm upon binding DNA. Generally, both the cytoplasm and nuclei of eukaryotic cells show staining with hexidium iodide. We do not recommend its use as a nuclear stain since it also stains other subcelluar compartments.

Protocol

SDS

Catalog	Size	Price	Quantity
17562	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Hexidium Iodide *CAS 211566-66-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	201.041 µL	1.005 mL	2.01 mL	10.052 mL	20.104 mL
5 mM	40.208 µL	201.041 µL	402.083 µL	2.01 mL	4.021 mL
10 mM	20.104 µL	100.521 µL	201.041 µL	1.005 mL	2.01 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	497.41 g/mol	=	mL	x	mM	=	mmol

References

View all 13 references: Citation Explorer

Novel model for multispecies biofilms that uses rigid gas-permeable lenses

Authors:

Peyyala R, Kirakodu SS, Ebersole JL, Novak KF.

Journal:

Appl Environ Microbiol (2011): 3413

Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum

Authors:

Linhova M, Patakova P, Lipovsky J, Fribert P, Paulova L, Rychtera M, Melzoch K.

Journal:

Folia Microbiol (Praha) (2010): 340

A flow cytometric technique for quantification and differentiation of bacteria in bulk tank milk

Authors:

Holm C, Mathiasen T, Jespersen L.

Journal:

J Appl Microbiol (2004): 935

Metabolic activity in filamentous fungi can be analysed by flow cytometry

Authors:

Bradner JR, Nevalainen KM.

Journal:

J Microbiol Methods (2003): 193

Potential for broad applications of flow cytometry and fluorescence techniques in microbiological and somatic cell analyses of milk

Authors:

Gunasekera TS, Veal DA, Attfield PV.

Journal:

Int J Food Microbiol (2003): 269

Physical properties

Molecular weight	497.41
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134
CAS	211566-66-4

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Nucleic Acid Detection Probes & Assay Kits

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Page updated on October 28, 2025