HIS Lite™ Cy3 Tris NTA Chelator
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1661.81 |
Solvent | Water |
Spectral properties
Correction Factor (260 nm) | 0.07 |
Correction Factor (280 nm) | 0.073 |
Extinction coefficient (cm -1 M -1) | 1500001 |
Excitation (nm) | 555 |
Emission (nm) | 569 |
Quantum yield | 0.151 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
Overview | SDSProtocol |
Molecular weight 1661.81 | Correction Factor (260 nm) 0.07 | Correction Factor (280 nm) 0.073 | Extinction coefficient (cm -1 M -1) 1500001 | Excitation (nm) 555 | Emission (nm) 569 | Quantum yield 0.151 |
Cy3-Tris NTA compound is a sensitive fluorescent chelator for detecting polyhistidine-labeled proteins in cells, solution, and solid surfaces with the addition of certain metal ions (such as Ni2+, Co2+ etc.) as a mediator. It is the chelator of our HIS Lite™ Cy3 Tris NTA-Ni Complex (#12620). Fluorescent tris-NTA compounds provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA conjugated fluorophores form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enables the selective labeling of proteins in cell lysates and on the surface of live cells. Fluorescent tris-NTA conjugates can be applied for the analysis of a ternary protein complex in solution and on surfaces. In combination with other color tris-NTA compounds (such as #12655 and #12657), it can be used for multicolor analysis of polyhistidine-tagged proteins. The transition metal ions (e.g., Ni2+)-mediated complexation of polyhistidine-labeled proteins with fluorescent tris-NTA conjugates provides a sensitive reporter for detecting and monitoring protein-protein interactions in real time.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of HIS Lite™ Cy3 Tris NTA Chelator to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 60.175 µL | 300.877 µL | 601.754 µL | 3.009 mL | 6.018 mL |
5 mM | 12.035 µL | 60.175 µL | 120.351 µL | 601.754 µL | 1.204 mL |
10 mM | 6.018 µL | 30.088 µL | 60.175 µL | 300.877 µL | 601.754 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.07 |
Correction Factor (280 nm) | 0.073 |
Extinction coefficient (cm -1 M -1) | 1500001 |
Excitation (nm) | 555 |
Emission (nm) | 569 |
Quantum yield | 0.151 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
HIS Lite™ Cy5 Tris NTA Chelator | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
HIS Lite™ Cy3 Bis NTA Chelator | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
HIS Lite™ OG488-Tris NTA Chelator | 498 | 526 | 76000 | - | 0.31 | 0.12 |
Images
References
View all 50 references: Citation Explorer
A poly-histidine motif of HOXA1 is involved in regulatory interactions with cysteine-rich proteins.
Authors: Marchese, Damien and Guislain, Florent and Pringels, Tamara and Bridoux, Laure and Rezsohazy, René
Journal: Biochimica et biophysica acta. Gene regulatory mechanisms (2024): 194993
Authors: Marchese, Damien and Guislain, Florent and Pringels, Tamara and Bridoux, Laure and Rezsohazy, René
Journal: Biochimica et biophysica acta. Gene regulatory mechanisms (2024): 194993
Improving the Performance of Selective Solid-State Nanopore Sensing Using a Polyhistidine-Tagged Monovalent Streptavidin.
Authors: Abu Jalboush, Sara and Wadsworth, Ian D and Sethi, Komal and Rogers, LeAnn C and Hollis, Thomas and Hall, Adam R
Journal: ACS sensors (2024): 1602-1610
Authors: Abu Jalboush, Sara and Wadsworth, Ian D and Sethi, Komal and Rogers, LeAnn C and Hollis, Thomas and Hall, Adam R
Journal: ACS sensors (2024): 1602-1610
Film-forming, stable, conductive composites of polyhistidine/graphene oxide for electrochemical quantification of trace Pb2.
Authors: Yang, Zhe-Han and Lei, Xin and Jiang, Guangming and Zhang, Xianming
Journal: RSC advances (2023): 15274-15279
Authors: Yang, Zhe-Han and Lei, Xin and Jiang, Guangming and Zhang, Xianming
Journal: RSC advances (2023): 15274-15279
Efficient Purification of Polyhistidine-Tagged Recombinant Proteins Using Functionalized Corundum Particles.
Authors: Völzke, Jule L and Smatty, Sarah and Döring, Sarah and Ewald, Shireen and Oelze, Marcus and Fratzke, Franziska and Flemig, Sabine and Konthur, Zoltán and Weller, Michael G
Journal: Biotech (Basel (Switzerland)) (2023)
Authors: Völzke, Jule L and Smatty, Sarah and Döring, Sarah and Ewald, Shireen and Oelze, Marcus and Fratzke, Franziska and Flemig, Sabine and Konthur, Zoltán and Weller, Michael G
Journal: Biotech (Basel (Switzerland)) (2023)
Variations in the poly-histidine repeat motif of HOXA1 contribute to bicuspid aortic valve in mouse and zebrafish.
Authors: Odelin, Gaëlle and Faucherre, Adèle and Marchese, Damien and Pinard, Amélie and Jaouadi, Hager and Le Scouarnec, Solena and , and Chiarelli, Raphaël and Achouri, Younes and Faure, Emilie and Herbane, Marine and Théron, Alexis and Avierinos, Jean-François and Jopling, Chris and Collod-Béroud, Gwenaëlle and Rezsohazy, René and Zaffran, Stéphane
Journal: Nature communications (2023): 1543
Authors: Odelin, Gaëlle and Faucherre, Adèle and Marchese, Damien and Pinard, Amélie and Jaouadi, Hager and Le Scouarnec, Solena and , and Chiarelli, Raphaël and Achouri, Younes and Faure, Emilie and Herbane, Marine and Théron, Alexis and Avierinos, Jean-François and Jopling, Chris and Collod-Béroud, Gwenaëlle and Rezsohazy, René and Zaffran, Stéphane
Journal: Nature communications (2023): 1543
Structure, dynamics and transferability of the metal-dependent polyhistidine tetramerization motif TetrHis for single-chain Fv antibodies.
Authors: Healey, Robert D and Couillaud, Louise and Hoh, François and Mouhand, Assia and Fouillen, Aurelien and Couvineau, Pierre and Granier, Sébastien and Leyrat, Cédric
Journal: Communications chemistry (2023): 160
Authors: Healey, Robert D and Couillaud, Louise and Hoh, François and Mouhand, Assia and Fouillen, Aurelien and Couvineau, Pierre and Granier, Sébastien and Leyrat, Cédric
Journal: Communications chemistry (2023): 160
Integrated tandem affinity protein purification using the polyhistidine plus extra 4 amino acids (HiP4) tag system.
Authors: Ino, Yoko and Yamaoka, Yutaro and Tanaka, Kiho and Miyakawa, Kei and Nishi, Mayuko and Hatayama, Yasuyoshi and Kimura, Hirokazu and Kimura, Yayoi and Ryo, Akihide
Journal: Proteomics (2023): e2200334
Authors: Ino, Yoko and Yamaoka, Yutaro and Tanaka, Kiho and Miyakawa, Kei and Nishi, Mayuko and Hatayama, Yasuyoshi and Kimura, Hirokazu and Kimura, Yayoi and Ryo, Akihide
Journal: Proteomics (2023): e2200334
Investigation of metal interactions with YrpE protein of Bacillus subtilis by a polyhistidine peptide model.
Authors: Bellotti, Denise and Leveraro, Silvia and Hecel, Aleksandra and Remelli, Maurizio
Journal: Analytical biochemistry (2023): 115315
Authors: Bellotti, Denise and Leveraro, Silvia and Hecel, Aleksandra and Remelli, Maurizio
Journal: Analytical biochemistry (2023): 115315
Efficient Delivery of Globotriaosylceramide Synthase siRNA using Polyhistidine-Incorporated Lipid Nanoparticles.
Authors: Kim, In Gyu and Jung, Won Ho and You, Gayeon and Lee, Hyukjin and Shin, Yoo Jin and Lim, Sun Woo and Chung, Byung Ha and Mok, Hyejung
Journal: Macromolecular bioscience (2023): e2200423
Authors: Kim, In Gyu and Jung, Won Ho and You, Gayeon and Lee, Hyukjin and Shin, Yoo Jin and Lim, Sun Woo and Chung, Byung Ha and Mok, Hyejung
Journal: Macromolecular bioscience (2023): e2200423
Effects of adding poly-histidine tag on stability, antimicrobial activity and safety of recombinant buforin I expressed in periplasmic space of Escherichia coli.
Authors: Roshanak, Sahar and Yarabbi, Hanieh and Shahidi, Fakhri and Tabatabaei Yazdi, Farideh and Movaffagh, Jebraeil and Javadmanesh, Ali
Journal: Scientific reports (2023): 5508
Authors: Roshanak, Sahar and Yarabbi, Hanieh and Shahidi, Fakhri and Tabatabaei Yazdi, Farideh and Movaffagh, Jebraeil and Javadmanesh, Ali
Journal: Scientific reports (2023): 5508