HIS Lite™ iFluor® 568 Tris NTA-Ni Complex

Fluorescent tris-NTA compounds provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA conjugated fluorophores form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enable the selective labeling of proteins in cell lysates and on the surface of live cells. Fluorescent tris-NTA conjugates can be applied for the analysis of a ternary protein complex in solution and on surfaces. The transition metal ions (e.g., Ni ion)-mediated complexation of polyhistidine-labeled proteins with fluorescent tris-NTA conjugates provides a sensitive reporter for detecting and monitoring protein-protein interactions in real time. This iFluor® 568 Tris NTA compound is used as an sensitive fluorescent probe for detecting polyhistidine-labeled proteins in cells, solution and solid surfaces. In combination with OG488-tris-NTA compound it can be used for multicolor analysis of polyhistidine-tagged proteins.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
12617	100 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of HIS Lite™ iFluor® 568 Tris NTA-Ni Complex to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	51.643 µL	258.215 µL	516.43 µL	2.582 mL	5.164 mL
5 mM	10.329 µL	51.643 µL	103.286 µL	516.43 µL	1.033 mL
10 mM	5.164 µL	25.822 µL	51.643 µL	258.215 µL	516.43 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1936.37 g/mol	=	mL	x	mM	=	mmol

Citations

View all 24 citations: Citation Explorer

Selective binding, magnetic separation and purification of histidine-tagged protein using biopolymer magnetic core-shell nanoparticles

Authors:

Zhou, Y., Yan, D., Yuan, S., Chen, Y., Fletcher, E. E., Shi, H., Han, B.

Journal:

Protein Expr Purif (2018): 5-11

Quantification of Histidine-Rich Protein 3 of Plasmodium falciparum

Authors:

Palani, B.

Journal:

Monoclon Antib Immunodiagn Immunother (2018): 87-90

A novel reverse micellar purification strategy for histidine tagged human interferon gamma (hIFN-gamma) protein from Pichia pastoris

Authors:

Prabhu, A. A., Purkayastha, A., M and al, B., Kumar, J. P., M and al, B. B., Veeranki, V. D.

Journal:

Int J Biol Macromol (2018): 2512-2524

Label-Free Direct Electrical Detection of a Histidine-Rich Protein with Sub-Femtomolar Sensitivity using an Organic Field-Effect Transistor

Authors:

Minamiki, T., Sasaki, Y., Tokito, S., Minami, T.

Journal:

ChemistryOpen (2017): 472-475

Controlling Protein Surface Orientation by Strategic Placement of Oligo-Histidine Tags

Authors:

Wasserberg, D., Cabanas-Danes, J., Prangsma, J., O'Mahony, S., Cazade, P. A., Tromp, E., Blum, C., Thompson, D., Huskens, J., Subramaniam, V., Jonkheijm, P.

Journal:

ACS Nano (2017): 9068-9083

Physical properties

Molecular weight	1936.37
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.34
Correction factor (280 nm)	0.15
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	568
Emission (nm)	587
Quantum yield	0.57 ¹

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Gel Imager
Excitation	Green laser
Emission	602/50 nm

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Page updated on October 13, 2025