HIS Lite™ OG488-Tris NTA Chelator
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1443.34 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.31 |
Correction Factor (280 nm) | 0.12 |
Extinction coefficient (cm -1 M -1) | 76000 |
Excitation (nm) | 498 |
Emission (nm) | 526 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
Overview | SDSProtocol |
Molecular weight 1443.34 | Correction Factor (260 nm) 0.31 | Correction Factor (280 nm) 0.12 | Extinction coefficient (cm -1 M -1) 76000 | Excitation (nm) 498 | Emission (nm) 526 |
HIS Lite™ OG488-Tris NTA Chelator is used as a sensitive green fluorescent probe for detecting polyhistidine-labeled proteins in combination with the addition of a heavy metal ion such as Ni2+ in cells, solution and solid surfaces. Fluorescent tris-NTA compounds provide an efficient method for site-specific and stable noncovalent fluorescence labeling of polyhistidine-tagged proteins. In contrast to the transient binding of conventional mono-NTA, the multivalent interaction of tris-NTA conjugated fluorophores form a much more stable complex with polyhistidine-tagged proteins. The high selectivity of tris-NTA compounds toward cumulated histidines enables the selective labeling of proteins in cell lysates and on the surface of live cells. Fluorescent tris-NTA conjugates can be applied for the analysis of a ternary protein complex in solution and on surfaces. AAT Bioquest also offers HIS Lite™ OG488-Tris NTA Complex that can be directly used for the specific and highly sensitive detection of His-tagged fusion proteins without the addition of a heavy metal ion. The transition metal ions (e.g., Ni ion)-mediated complexation of polyhistidine-labeled proteins with fluorescent tris-NTA conjugates provides a sensitive reporter for detecting and monitoring protein-protein interactions in real time.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of HIS Lite™ OG488-Tris NTA Chelator to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 69.284 µL | 346.419 µL | 692.837 µL | 3.464 mL | 6.928 mL |
5 mM | 13.857 µL | 69.284 µL | 138.567 µL | 692.837 µL | 1.386 mL |
10 mM | 6.928 µL | 34.642 µL | 69.284 µL | 346.419 µL | 692.837 µL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.31 |
Correction Factor (280 nm) | 0.12 |
Extinction coefficient (cm -1 M -1) | 76000 |
Excitation (nm) | 498 |
Emission (nm) | 526 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) |
HIS Lite™ Cy5 Tris NTA Chelator | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 | 0.009 | 0.09 |
HIS Lite™ Cy3 Tris NTA Chelator | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 | - | - |
Images
References
View all 6 references: Citation Explorer
Heterologous overexpression of active hexokinases from microsporidia Nosema bombycis and Nosema ceranae confirms their ability to phosphorylate host glucose.
Authors: Dolgikh, Viacheslav V and Tsarev, Alexander A and Timofeev, Sergey A and Zhuravlyov, Vladimir S
Journal: Parasitology research (2019): 1511-1518
Authors: Dolgikh, Viacheslav V and Tsarev, Alexander A and Timofeev, Sergey A and Zhuravlyov, Vladimir S
Journal: Parasitology research (2019): 1511-1518
Systematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags.
Authors: Bernier, Sarah C and Cantin, Line and Salesse, Christian
Journal: Protein expression and purification (2018): 92-106
Authors: Bernier, Sarah C and Cantin, Line and Salesse, Christian
Journal: Protein expression and purification (2018): 92-106
Expression, purification, and functional characterisation of flagellin, a TLR5-ligand.
Authors: Hajam, Irshad Ahmed and Dar, Pervaiz Ahmed and Sekar, Shanmugam Chandra and Nanda, Rajakishore and Kishore, Subodh and Bhanuprakash, Veerakyathappa and Ganesh, Kondabattula
Journal: Veterinaria italiana (2013): 181-6
Authors: Hajam, Irshad Ahmed and Dar, Pervaiz Ahmed and Sekar, Shanmugam Chandra and Nanda, Rajakishore and Kishore, Subodh and Bhanuprakash, Veerakyathappa and Ganesh, Kondabattula
Journal: Veterinaria italiana (2013): 181-6
Identification of the sequence encoding N-acetylneuraminate-9-phosphate phosphatase.
Authors: Maliekal, Pushpa and Vertommen, Didier and Delpierre, Ghislain and Van Schaftingen, Emile
Journal: Glycobiology (2006): 165-72
Authors: Maliekal, Pushpa and Vertommen, Didier and Delpierre, Ghislain and Van Schaftingen, Emile
Journal: Glycobiology (2006): 165-72
Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the filamentous fungus, Talaromyces emersonii.
Authors: Grassick, Alice and Murray, Patrick G and Thompson, Roisin and Collins, Catherine M and Byrnes, Lucy and Birrane, Gabriel and Higgins, Timothy M and Tuohy, Maria G
Journal: European journal of biochemistry (2004): 4495-506
Authors: Grassick, Alice and Murray, Patrick G and Thompson, Roisin and Collins, Catherine M and Byrnes, Lucy and Birrane, Gabriel and Higgins, Timothy M and Tuohy, Maria G
Journal: European journal of biochemistry (2004): 4495-506
Expression of functional melanocortin 1 receptors in insect cells.
Authors: Schiöth, H B and Kuusinen, A and Muceniece, R and Szardenings, M and Keinänen, K and Wikberg, J E
Journal: Biochemical and biophysical research communications (1996): 807-14
Authors: Schiöth, H B and Kuusinen, A and Muceniece, R and Szardenings, M and Keinänen, K and Wikberg, J E
Journal: Biochemical and biophysical research communications (1996): 807-14