Hoechst 33342 alkyne

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Physical properties

Molecular weight	547.66
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

Overview SDS Protocol

See also: Click Chemistry

Molecular weight

547.66

Hoechst 33342 alkyne is an excellent building block for developing site-specific nucleic acid probes via the well-known click chemistry, i.e., Copper-Catalyzed Azide–Alkyne Cycloaddition (CuAAC). It can be readily conjugated to azido-containing biomolecules (such as azido-modified antibodies, peptide azides and azido-modified oligos). Hoechst 33342 is a DNA-specific dye that binds to the minor groove of double-stranded DNA. Its ability to readily penetrate live cell membranes makes it suitable for staining live cells. This property allows researchers to monitor nuclear morphology and DNA content in living cells. Hoechst 33342 is commonly used in fluorescence microscopy to visualize the nucleus of cells. It selectively stains the DNA in the cell nucleus and is often used in combination with other fluorochromes to study cell structure and dynamics. In addition, Hoechst 33342 has also been used in the identification and isolation of stem cells based on their ability to efflux the dye. This property is exploited in a technique called side population analysis, commonly used in stem cell research.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Hoechst 33342 alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	182.595 µL	912.975 µL	1.826 mL	9.13 mL	18.26 mL
5 mM	36.519 µL	182.595 µL	365.19 µL	1.826 mL	3.652 mL
10 mM	18.26 µL	91.298 µL	182.595 µL	912.975 µL	1.826 mL

Molarity calculator

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Images

Figure 1. Hoechst 33342 alkyne is an excellent building block for developing site-specific nucleic acid probes via the well-known click chemistry, i.e., Copper-Catalyzed Azide–Alkyne Cycloaddition (CuAAC). It can be readily conjugated to azido-containing biomolecules (such as azido-modified antibodies, peptide azides and azido-modified oligos).

References

View all 2 references: Citation Explorer

Multivariate analysis of apoptotic markers versus cell cycle phase in living human cancer cells by microfluidic cytometry.
Authors: Akagi, Jin and Skommer, Joanna and Matuszek, Anna and Takeda, Kazuo and Fujimura, Yuu and Khoshmanesh, Khashayar and Kalantar-Zadeh, Kourosh and Mitchell, Arnan and Errington, Rachel and Smith, Paul J and Darzynkiewicz, Zbigniew and Wlodkowic, Donald
Journal: Proceedings of SPIE--the International Society for Optical Engineering (2013)

Lens epithelial cell protection by aminothiol WR-1065 and anetholedithiolethione from ionizing radiation.
Authors: Belkacémi, Y and Rat, P and Piel, G and Christen, M O and Touboul, E and Warnet, J M
Journal: International journal of cancer (2001): 15-26