Hoechst 33342 amine

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Physical properties

Molecular weight	495.63
Solvent	DMSO

Spectral properties

Excitation (nm)	352
Emission (nm)	454

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

Overview SDS Protocol

Molecular weight

495.63

Excitation (nm)

352

Emission (nm)

454

Hoechst 33342 amine is an excellent building block for developing site-specific nucleic acid probes. It can be readily conjugated to carbonyl-containing biomolecules (such as antibodies, peptides and oligos). Hoechst 33342 is a DNA-specific dye that binds to the minor groove of double-stranded DNA. It emits blue fluorescence when bound to DNA. Its excitation maximum is around 350 nm, and the emission maximum is approximately 460 nm. Its ability to readily penetrate live cell membranes makes it suitable for staining live cells. This property allows researchers to monitor nuclear morphology and DNA content in living cells. Hoechst 33342 is commonly used in fluorescence microscopy to visualize the nucleus of cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Hoechst 33342 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	201.763 µL	1.009 mL	2.018 mL	10.088 mL	20.176 mL
5 mM	40.353 µL	201.763 µL	403.527 µL	2.018 mL	4.035 mL
10 mM	20.176 µL	100.882 µL	201.763 µL	1.009 mL	2.018 mL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	352
Emission (nm)	454

Images

Figure 1. Hoechst 33342 amine is an excellent building block for developing site-specific nucleic acid probes. It can be readily conjugated to carbonyl-containing biomolecules (such as antibodies, peptides and oligos). Hoechst 33342 is a DNA-specific dye that binds to the minor groove of double-stranded DNA.

References

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Journal: Biomedicines (2022)

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Journal: Biochemical and biophysical research communications (2021): 167-173

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Authors: Gębczak, Katarzyna and Wiatrak, Benita and Fortuna, Wojciech
Journal: Materials (Basel, Switzerland) (2021)

Improvements in Flow Cytometry-Based Cytotoxicity Assay.
Authors: Wu, Xiaolong and Zhang, Ying and Li, Yutao and Schmidt-Wolf, Ingo G H
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2021): 680-688

Glembatumumab vedotin for patients with metastatic, gpNMB overexpressing, triple-negative breast cancer ("METRIC"): a randomized multicenter study.
Authors: Vahdat, Linda T and Schmid, Peter and Forero-Torres, Andres and Blackwell, Kimberly and Telli, Melinda L and Melisko, Michelle and Möbus, Volker and Cortes, Javier and Montero, Alberto J and Ma, Cynthia and Nanda, Rita and Wright, Gail S and He, Yi and Hawthorne, Thomas and Bagley, Rebecca G and Halim, Abdel-Baset and Turner, Christopher D and Yardley, Denise A
Journal: NPJ breast cancer (2021): 57

Phlogiellus bundokalbo spider venom: cytotoxic fractions against human lung adenocarcinoma (A549) cells.
Authors: Mayor, Anna Beatriz R and Guevarra, Leonardo A and Santiago-Bautista, Myla R and Santiago, Librado A
Journal: The journal of venomous animals and toxins including tropical diseases (2020): e20190104

Targeted cell imaging properties of a deep red luminescent iridium(iii) complex conjugated with a c-Myc signal peptide.
Authors: Day, Adam H and Übler, Martin H and Best, Hannah L and Lloyd-Evans, Emyr and Mart, Robert J and Fallis, Ian A and Allemann, Rudolf K and Al-Wattar, Eman A H and Keymer, Nathaniel I and Buurma, Niklaas J and Pope, Simon J A
Journal: Chemical science (2020): 1599-1606