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Hoechst 33342 azide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 521.63 |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 352 |
| Emission (nm) | 454 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| Hoechst 33342 amine |
| Hoechst 33342 maleimide |
| Hoechst 33342 alkyne |
| Overview |  SDSProtocol |
See also: Click Chemistry
Molecular weight 521.63 | Excitation (nm) 352 | Emission (nm) 454 |
Hoechst 33342 azide is an excellent building block for developing site-specific nucleic acid probes via the well-known click chemistry, i.e., Copper-Catalyzed Azide–Alkyne Cycloaddition (CuAAC). It can be readily conjugated to alkyne-containing biomolecules (such as DBCO-modified antibodies, alkynyl-modified peptides and alkynyl-modified oligos). Hoechst 33342 is a DNA-specific dye that binds to the minor groove of double-stranded DNA. It emits blue fluorescence when bound to DNA. Its excitation maximum is around 350 nm, and the emission maximum is approximately 460 nm. Its ability to readily penetrate live cell membranes makes it suitable for staining live cells. This property allows researchers to monitor nuclear morphology and DNA content in living cells. Hoechst 33342 has also been used in the identification and isolation of stem cells based on their ability to efflux the dye. This property is exploited in a technique called side population analysis, commonly used in stem cell research.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Hoechst 33342 azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 191.707 µL | 958.534 µL | 1.917 mL | 9.585 mL | 19.171 mL |
| 5 mM | 38.341 µL | 191.707 µL | 383.414 µL | 1.917 mL | 3.834 mL |
| 10 mM | 19.171 µL | 95.853 µL | 191.707 µL | 958.534 µL | 1.917 mL |
Molarity calculator
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Images
Figure 1. Hoechst 33342 azide is an excellent building block for developing site-specific nucleic acid probes via the well-known click chemistry, i.e., Copper-Catalyzed Azide–Alkyne Cycloaddition (CuAAC). It can be readily conjugated to alkyne-containing biomolecules (such as DBCO-modified antibodies, alkynyl-modified peptides and alkynyl-modified oligos).
References
View all 50 references: Citation Explorer
Investigating the antitumor effects of a novel ruthenium (II) complex on malignant melanoma cells: An NMR-based metabolomic approach.
Authors: Hussan, Ayesha and Moyo, Brenden and Amenuvor, Gershon and Meyer, Debra and Sitole, Lungile
Journal: Biochemical and biophysical research communications (2023): 149169
Authors: Hussan, Ayesha and Moyo, Brenden and Amenuvor, Gershon and Meyer, Debra and Sitole, Lungile
Journal: Biochemical and biophysical research communications (2023): 149169
Temporal relationship of the orphan receptor TR3 translocation and expression with zinc-induced apoptosis in prostate cancer cells.
Authors: Ma, Ding and Guo, Yuchen and Fu, Yongqiang and Wu, Jinfeng and Ren, Ruimin
Journal: Translational andrology and urology (2023): 444-454
Authors: Ma, Ding and Guo, Yuchen and Fu, Yongqiang and Wu, Jinfeng and Ren, Ruimin
Journal: Translational andrology and urology (2023): 444-454
SiR-DNA/SiR-Hoechst-induced chromosome entanglement generates severe anaphase bridges and DNA damage.
Authors: Rajendraprasad, Girish and Rodriguez-Calado, Sergi and Barisic, Marin
Journal: Life science alliance (2023)
Authors: Rajendraprasad, Girish and Rodriguez-Calado, Sergi and Barisic, Marin
Journal: Life science alliance (2023)
Fluorescence Spectroscopic Studies to Evaluate Binding Interaction between Hoechst 33258 and Bilirubin.
Authors: Singh Chauhan, Srishti and Mohan Murari, Bhaskar
Journal: Journal of fluorescence (2023)
Authors: Singh Chauhan, Srishti and Mohan Murari, Bhaskar
Journal: Journal of fluorescence (2023)
Vibsane-type diterpenoids from Viburnum odoratissimum inhibit hepatocellular carcinoma cells via the PI3K/AKT pathway.
Authors: Xue, Xiao-Bian and Lv, Tian-Ming and Hou, Jiao-Yang and Li, Dan-Qi and Huang, Xiao-Xiao and Song, Shao-Jiang and Yao, Guo-Dong
Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (2023): 154499
Authors: Xue, Xiao-Bian and Lv, Tian-Ming and Hou, Jiao-Yang and Li, Dan-Qi and Huang, Xiao-Xiao and Song, Shao-Jiang and Yao, Guo-Dong
Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology (2023): 154499
Interaction of Hoechst 33342 with POPC Membranes at Different pH Values.
Authors: Cordeiro, Margarida M and Filipe, Hugo A L and Santos, Patrícia Dos and Samelo, Jaime and Ramalho, João P Prates and Loura, Luís M S and Moreno, Maria J
Journal: Molecules (Basel, Switzerland) (2023)
Authors: Cordeiro, Margarida M and Filipe, Hugo A L and Santos, Patrícia Dos and Samelo, Jaime and Ramalho, João P Prates and Loura, Luís M S and Moreno, Maria J
Journal: Molecules (Basel, Switzerland) (2023)
Overexpression of the ABC Transporter BmrA Within Intracellular Caveolae in Escherichia coli.
Authors: Gobet, Alexia and Jaxel, Christine and Magnard, Sandrine and Garrigos, Manuel and Orlowski, Stéphane and Jamin, Nadège and Falson, Pierre and Chaptal, Vincent
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 79-89
Authors: Gobet, Alexia and Jaxel, Christine and Magnard, Sandrine and Garrigos, Manuel and Orlowski, Stéphane and Jamin, Nadège and Falson, Pierre and Chaptal, Vincent
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 79-89
Lingguizhugan decoction protects PC12 cells against Aβ25-35-induced oxidative stress and neuroinflammation by modulating NF-κB/MAPK signaling pathways.
Authors: Han, Jing and Zhang, Haotian and Zhang, Yu and Zhang, Zan and Yu, Maomao and Wang, Sijie and Han, Fei
Journal: Journal of ethnopharmacology (2022): 115194
Authors: Han, Jing and Zhang, Haotian and Zhang, Yu and Zhang, Zan and Yu, Maomao and Wang, Sijie and Han, Fei
Journal: Journal of ethnopharmacology (2022): 115194
A novel combined fluorescent probe staining method for circulating tumor cell identification.
Authors: Wang, Huansheng and Pei, Fajun and Li, Hao and Li, Bing and Han, Shili and Yu, Huajie and Li, Sheng
Journal: Annals of translational medicine (2022): 50
Authors: Wang, Huansheng and Pei, Fajun and Li, Hao and Li, Bing and Han, Shili and Yu, Huajie and Li, Sheng
Journal: Annals of translational medicine (2022): 50
Symmetry dual functional pyrimidine-BODIPY probes for imaging targeting and activity study.
Authors: Xu, Shuping and Liu, Ying and Wang, Zhou and He, Aolin and Jin, Guofan
Journal: Frontiers in chemistry (2022): 977008
Authors: Xu, Shuping and Liu, Ying and Wang, Zhou and He, Aolin and Jin, Guofan
Journal: Frontiers in chemistry (2022): 977008