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Hoechst 33342 maleimide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 646.75 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| Hoechst 33342 amine |
| Hoechst 33342 azide |
| Hoechst 33342 alkyne |
| Overview |  SDSProtocol |
Molecular weight 646.75 |
Hoechst 33342 maleimide is an excellent building block for developing site-specific nucleic acid probes. It can be readily conjugated to thiol-containing biomolecules (such as antibodies, peptides and thiol-modified oligos). Hoechst 33342 is a DNA-specific dye that binds to the minor groove of double-stranded DNA. It emits blue fluorescence when bound to DNA. Its excitation maximum is around 350 nm, and the emission maximum is approximately 460 nm. Its ability to readily penetrate live cell membranes makes it suitable for staining live cells. Hoechst 33342 is commonly used in fluorescence microscopy to visualize the nucleus of cells. It selectively stains the DNA in the cell nucleus and is often used in combination with other fluorochromes to study cell structure and dynamics. Hoechst 33342 is used for cell cycle analysis in flow cytometry. By staining DNA, it allows researchers to distinguish between different phases of the cell cycle, such as G1, S, and G2/M.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Hoechst 33342 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 154.619 µL | 773.096 µL | 1.546 mL | 7.731 mL | 15.462 mL |
| 5 mM | 30.924 µL | 154.619 µL | 309.239 µL | 1.546 mL | 3.092 mL |
| 10 mM | 15.462 µL | 77.31 µL | 154.619 µL | 773.096 µL | 1.546 mL |
Molarity calculator
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Images
Figure 1. Hoechst 33342 maleimide is an excellent building block for developing site-specific nucleic acid probes. It can be readily conjugated to thiol-containing biomolecules (such as antibodies, peptides and thiol-modified oligos).
References
View all 50 references: Citation Explorer
AQP8 may affect glioma proliferation and growth by regulating GSK-3β phosphorylation and nuclear transport of β-catenin.
Authors: Cai, Ziling and Shen, Zihao and Zhao, Jing and Zhang, Hao and Guo, Zhen and Xia, Qingqian and Liang, Hang and Liu, Junnan and Tan, Lihao and Sheng, Huajun and Zhu, Shujuan
Journal: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuros (2023): 333-345
Authors: Cai, Ziling and Shen, Zihao and Zhao, Jing and Zhang, Hao and Guo, Zhen and Xia, Qingqian and Liang, Hang and Liu, Junnan and Tan, Lihao and Sheng, Huajun and Zhu, Shujuan
Journal: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuros (2023): 333-345
EXOSC10 is a novel hepatocellular carcinoma prognostic biomarker: a comprehensive bioinformatics analysis and experiment verification.
Authors: Meng, Zhi-Yong and Fan, Yu-Chun and Zhang, Chao-Sheng and Zhang, Lin-Li and Wu, Tong and Nong, Min-Yu and Wang, Tian and Chen, Chuang and Jiang, Li-He
Journal: PeerJ (2023): e15860
Authors: Meng, Zhi-Yong and Fan, Yu-Chun and Zhang, Chao-Sheng and Zhang, Lin-Li and Wu, Tong and Nong, Min-Yu and Wang, Tian and Chen, Chuang and Jiang, Li-He
Journal: PeerJ (2023): e15860
Antimycin A induced apoptosis in HCT-116 colorectal cancer cells through the up- and downregulation of multiple signaling pathways.
Authors: Kabir, Syed Rashel and Islam, Tofazzal
Journal: Medical oncology (Northwood, London, England) (2022): 51
Authors: Kabir, Syed Rashel and Islam, Tofazzal
Journal: Medical oncology (Northwood, London, England) (2022): 51
Hypnea musciformis-mediated Ag/AgCl-NPs inhibit pathogenic bacteria, HCT-116 and MCF-7 cells' growth in vitro and Ehrlich ascites carcinoma cells in vivo in mice.
Authors: Ghose, Rita and Asaduzzaman, A K M and Hasan, Imtiaj and Kabir, Syed Rashel
Journal: IET nanobiotechnology (2022): 49-60
Authors: Ghose, Rita and Asaduzzaman, A K M and Hasan, Imtiaj and Kabir, Syed Rashel
Journal: IET nanobiotechnology (2022): 49-60
Role of ORAI calcium release-activated calcium modulator 1 (ORAI1) on neutrophil extracellular trap formation in dairy cows with subclinical hypocalcemia.
Authors: Zhang, Bingbing and Ma, Xinru and Loor, Juan J and Jiang, Qianming and Guo, Han and Zhang, Wei and Li, Ming and Lv, Xinquan and Yin, Yufeng and Wen, Jianan and Wang, Jingjing and Xu, Chuang and Yang, Wei
Journal: Journal of dairy science (2022): 3394-3404
Authors: Zhang, Bingbing and Ma, Xinru and Loor, Juan J and Jiang, Qianming and Guo, Han and Zhang, Wei and Li, Ming and Lv, Xinquan and Yin, Yufeng and Wen, Jianan and Wang, Jingjing and Xu, Chuang and Yang, Wei
Journal: Journal of dairy science (2022): 3394-3404
Collection and in vitro maturation of Mazama gouazoubira (brown brocket deer) oocytes obtained after ovarian stimulation.
Authors: Rola, Luciana Diniz and Zanetti, Eveline Dos Santos and Del Collado, Maite and Peroni, Ellen de Fátima Carvalho and Duarte, José Maurício Barbanti
Journal: Zygote (Cambridge, England) (2021): 216-222
Authors: Rola, Luciana Diniz and Zanetti, Eveline Dos Santos and Del Collado, Maite and Peroni, Ellen de Fátima Carvalho and Duarte, José Maurício Barbanti
Journal: Zygote (Cambridge, England) (2021): 216-222
Phytochemicals from Ajwa dates pulp extract induce apoptosis in human triple-negative breast cancer by inhibiting AKT/mTOR pathway and modulating Bcl-2 family proteins.
Authors: Khan, Mohsin Ali and Siddiqui, Sahabjada and Ahmad, Imran and Singh, Romila and Mishra, Durga Prasad and Srivastava, Anand Narain and Ahmad, Rumana
Journal: Scientific reports (2021): 10322
Authors: Khan, Mohsin Ali and Siddiqui, Sahabjada and Ahmad, Imran and Singh, Romila and Mishra, Durga Prasad and Srivastava, Anand Narain and Ahmad, Rumana
Journal: Scientific reports (2021): 10322
Clinical significance of cancer stem cell markers in lung carcinoma.
Authors: Zhang, Liang and Shen, Lianglan and Wu, Dijun
Journal: Acta biochimica Polonica (2021): 187-191
Authors: Zhang, Liang and Shen, Lianglan and Wu, Dijun
Journal: Acta biochimica Polonica (2021): 187-191
Association of ALDH1A1-NEK-2 axis in cisplatin resistance in ovarian cancer cells.
Authors: Uddin, Md Hafiz and Kim, Boyun and Cho, Untack and Azmi, Asfar S and Song, Yong Sang
Journal: Heliyon (2020): e05442
Authors: Uddin, Md Hafiz and Kim, Boyun and Cho, Untack and Azmi, Asfar S and Song, Yong Sang
Journal: Heliyon (2020): e05442
Differentiation of CD45‑/CD31+ lung side population cells into endothelial and smooth muscle cells in vitro.
Authors: Xu, Yang and Sun, Ping and Wang, Jian-Yu and Li, Zong-Ze and Gao, Rui-Lan and Wang, Xue-Zhe and Phillips, William D and Liang, Simon X
Journal: International journal of molecular medicine (2019): 1128-1138
Authors: Xu, Yang and Sun, Ping and Wang, Jian-Yu and Li, Zong-Ze and Gao, Rui-Lan and Wang, Xue-Zhe and Phillips, William D and Liang, Simon X
Journal: International journal of molecular medicine (2019): 1128-1138