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Hoechst 33342
Ultrapure Grade
Hoechst 33342 Ultrapure Grade is a cell-permeable blue fluorescent dye that binds to AT-rich regions in the minor groove of DNA, enabling live cell nuclear staining, cell cycle analysis, and identification of side population stem cells.
  • Optimal UV Brightness: Maximum fluorescence under UV or near-UV excitation for clear nuclear visualization
  • Low Background Noise: Minimal cytoplasmic staining ensures clean nuclear segmentation in imaging applications
  • Live-Cell Compatible: Cell-permeable design allows real-time nuclear morphology and cell cycle studies
  • Sigma/Invitrogen Equivalent: Industry-standard performance with enhanced purity and batch-to-batch consistency
Hoechst 33342 spectrum. Hoechst 33342 is a fluorescent compound with an excitation peak at 352 nm and an emission peak at 454 nm. Other spectra of interest include: Hoechst 33258, DAPI (4,6-Diamidino-2-phenylindole), and Hoechst 34580. Hoechst 33342 belongs to the following categories: Cell Cycle Assays, Nucleus, Fluorescence Activated Cell Sorting (FACS), Hoechst DNA Stains for Live and Fixed Cells, Immunohistochemistry (IHC), and Nucleic Acid Building Blocks.
Hoechst 33342 spectrum. Hoechst 33342 is a fluorescent compound with an excitation peak at 352 nm and an emission peak at 454 nm. Other spectra of interest include: Hoechst 33258, DAPI (4,6-Diamidino-2-phenylindole), and Hoechst 34580. Hoechst 33342 belongs to the following categories: Cell Cycle Assays, Nucleus, Fluorescence Activated Cell Sorting (FACS), Hoechst DNA Stains for Live and Fixed Cells, Immunohistochemistry (IHC), and Nucleic Acid Building Blocks.
CatalogSize
Price
Quantity
17530100 mg
Price
175331 g
Price
 
Physical properties

Molecular weight561.93
SolventWater
Spectral properties

Excitation (nm)352
Emission (nm)454
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
CAS875756-97-1
Instrument settings

Fluorescence microscope
Excitation350 nm
Emission461 nm
Recommended plateBlack wall/clear bottom
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Page updated on January 22, 2025