Hoechst 34580 *CAS 911004-45-0*

Hoechst 34580

CAS 911004-45-0

The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Hoechst 34580 can be excited by violet laser at 405 nm, and emit blue/cyan fluorescence light around an emission maximum at ~440 nm. It is cell-permeable and provide an excellent tool for flow cytometers equiped with a 405 nm violet laser source.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17537	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Hoechst 34580 *CAS 911004-45-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	178.269 µL	891.345 µL	1.783 mL	8.913 mL	17.827 mL
5 mM	35.654 µL	178.269 µL	356.538 µL	1.783 mL	3.565 mL
10 mM	17.827 µL	89.135 µL	178.269 µL	891.345 µL	1.783 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	560.95 g/mol	=	mL	x	mM	=	mmol

Citations

View all 5 citations: Citation Explorer

A circadian clock translational control mechanism targets specific mRNAs to cytoplasmic messenger ribonucleoprotein granules

Authors:

Castillo, Kathrina D and Wu, Cheng and Ding, Zhaolan and Lopez-Garcia, Osiris K and Rowlinson, Emma and Sachs, Matthew S and Bell-Pedersen, Deborah

Journal:

Cell Reports (2022): 111879

MDA5 Induces a Stronger Interferon Response than RIG-I to GCRV Infection through a Mechanism Involving the Phosphorylation and Dimerization of IRF3 and IRF7 in CIK Cells

Authors:

Wan, Quanyuan and Yang, Chunrong and Rao, Youliang and Liao, Zhiwei and Su, Jianguo

Journal:

Frontiers in Immunology (2017)

Grafting of Ring-Opened Cyclopropylamine thin films on Silicon (100) Hydride via UV Photoionization

Authors:

Tung, Joline and Ching, Jing Yuan and Ng, Yoke Mooi and Tew, Lih Shin and Khung, Yit Lung

Journal:

ACS Applied Materials & Interfaces (2017)

Dual-functionalized graphene oxide for enhanced siRNA delivery to breast cancer cells

Authors:

Imani, Rana and Shao, Wei and Taherkhani, Samira and Emami, Shahriar Hojjati and Prakash, Satya and Faghihi, Shahab

Journal:

Colloids and Surfaces B: Biointerfaces (2016): 315--325

A C-terminal acidic domain regulates degradation of the transcriptional coactivator Bob1

Authors:

Lindner, John M and Wong, Christina SF and Möller, Andreas and Nielsen, Peter J

Journal:

Molecular and cellular biology (2013): 4628--4640

References

View all 42 references: Citation Explorer

Usefulness of a triple fluorochrome combination Merocyanine 540/Yo-Pro 1/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls

Authors:

Hallap T, Nagy S, Jaakma U, Johannisson A, Rodriguez-Martinez H.

Journal:

Theriogenology (2006): 1122

Fatty acid synthase and its mRNA concentrations are decreased at different times following Hoechst 33342-induced apoptosis in BC3H-1 myocytes

Authors:

Zhang X, Kiechle FL.

Journal:

Ann Clin Lab Sci (2006): 185

Evidence for a qualitative hierarchy within the Hoechst-33342 'side population' (SP) of murine bone marrow cells

Authors:

Robinson SN, Seina SM, Gohr JC, Kuszynski CA, Sharp JG.

Journal:

Bone Marrow Transplant (2005): 807

Proton motive force-dependent Hoechst 33342 transport by the ABC transporter LmrA of Lactococcus lactis

Authors:

van den Berg van Saparoea HB, Lubelski J, van Merkerk R, Mazurkiewicz PS, Driessen AJ.

Journal:

Biochemistry (2005): 16931

Role of topoisomerases in cytotoxicity induced by DNA ligand Hoechst-33342 and UV-C in a glioma cell line

Authors:

Singh S, Dwarakanath BS, Lazar Mathew T.

Journal:

Indian J Exp Biol (2005): 313

Physical properties

Molecular weight	560.95
Solvent	Water

Spectral properties

Excitation (nm)	371
Emission (nm)	438

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134
CAS	911004-45-0

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