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Hoechst 34580 *CAS 911004-45-0*
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The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Hoechst 34580 can be excited by violet laser at 405 nm, and emit blue/cyan fluorescence light around an emission maximum at ~440 nm. It is cell-permeable and provide an excellent tool for flow cytometers equiped with a 405 nm violet laser source.
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Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Hoechst 34580 *CAS 911004-45-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 178.269 µL | 891.345 µL | 1.783 mL | 8.913 mL | 17.827 mL |
| 5 mM | 35.654 µL | 178.269 µL | 356.538 µL | 1.783 mL | 3.565 mL |
| 10 mM | 17.827 µL | 89.135 µL | 178.269 µL | 891.345 µL | 1.783 mL |
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Hoechst 33258 *CAS 23491-45-4* | 352 | 454 | 460001 | 0.03401 |
| Hoechst 33258 *20 mM solution in water* | 352 | 454 | 460001 | 0.03401 |
| Hoechst 33342 *Ultrapure Grade* | 352 | 454 | - | - |
| Hoechst 33342 *20 mM solution in water* | 352 | 454 | - | - |
Citations
View all 5 citations: Citation Explorer
A circadian clock translational control mechanism targets specific mRNAs to cytoplasmic messenger ribonucleoprotein granules
Authors: Castillo, Kathrina D and Wu, Cheng and Ding, Zhaolan and Lopez-Garcia, Osiris K and Rowlinson, Emma and Sachs, Matthew S and Bell-Pedersen, Deborah
Journal: Cell Reports (2022): 111879
Authors: Castillo, Kathrina D and Wu, Cheng and Ding, Zhaolan and Lopez-Garcia, Osiris K and Rowlinson, Emma and Sachs, Matthew S and Bell-Pedersen, Deborah
Journal: Cell Reports (2022): 111879
MDA5 Induces a Stronger Interferon Response than RIG-I to GCRV Infection through a Mechanism Involving the Phosphorylation and Dimerization of IRF3 and IRF7 in CIK Cells
Authors: Wan, Quanyuan and Yang, Chunrong and Rao, Youliang and Liao, Zhiwei and Su, Jianguo
Journal: Frontiers in Immunology (2017)
Authors: Wan, Quanyuan and Yang, Chunrong and Rao, Youliang and Liao, Zhiwei and Su, Jianguo
Journal: Frontiers in Immunology (2017)
Grafting of Ring-Opened Cyclopropylamine thin films on Silicon (100) Hydride via UV Photoionization
Authors: Tung, Joline and Ching, Jing Yuan and Ng, Yoke Mooi and Tew, Lih Shin and Khung, Yit Lung
Journal: ACS Applied Materials & Interfaces (2017)
Authors: Tung, Joline and Ching, Jing Yuan and Ng, Yoke Mooi and Tew, Lih Shin and Khung, Yit Lung
Journal: ACS Applied Materials & Interfaces (2017)
Dual-functionalized graphene oxide for enhanced siRNA delivery to breast cancer cells
Authors: Imani, Rana and Shao, Wei and Taherkhani, Samira and Emami, Shahriar Hojjati and Prakash, Satya and Faghihi, Shahab
Journal: Colloids and Surfaces B: Biointerfaces (2016): 315--325
Authors: Imani, Rana and Shao, Wei and Taherkhani, Samira and Emami, Shahriar Hojjati and Prakash, Satya and Faghihi, Shahab
Journal: Colloids and Surfaces B: Biointerfaces (2016): 315--325
A C-terminal acidic domain regulates degradation of the transcriptional coactivator Bob1
Authors: Lindner, John M and Wong, Christina SF and Möller, Andreas and Nielsen, Peter J
Journal: Molecular and cellular biology (2013): 4628--4640
Authors: Lindner, John M and Wong, Christina SF and Möller, Andreas and Nielsen, Peter J
Journal: Molecular and cellular biology (2013): 4628--4640
References
View all 42 references: Citation Explorer
Usefulness of a triple fluorochrome combination Merocyanine 540/Yo-Pro 1/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls
Authors: Hallap T, Nagy S, Jaakma U, Johannisson A, Rodriguez-Martinez H.
Journal: Theriogenology (2006): 1122
Authors: Hallap T, Nagy S, Jaakma U, Johannisson A, Rodriguez-Martinez H.
Journal: Theriogenology (2006): 1122
Fatty acid synthase and its mRNA concentrations are decreased at different times following Hoechst 33342-induced apoptosis in BC3H-1 myocytes
Authors: Zhang X, Kiechle FL.
Journal: Ann Clin Lab Sci (2006): 185
Authors: Zhang X, Kiechle FL.
Journal: Ann Clin Lab Sci (2006): 185
Evidence for a qualitative hierarchy within the Hoechst-33342 'side population' (SP) of murine bone marrow cells
Authors: Robinson SN, Seina SM, Gohr JC, Kuszynski CA, Sharp JG.
Journal: Bone Marrow Transplant (2005): 807
Authors: Robinson SN, Seina SM, Gohr JC, Kuszynski CA, Sharp JG.
Journal: Bone Marrow Transplant (2005): 807
Proton motive force-dependent Hoechst 33342 transport by the ABC transporter LmrA of Lactococcus lactis
Authors: van den Berg van Saparoea HB, Lubelski J, van Merkerk R, Mazurkiewicz PS, Driessen AJ.
Journal: Biochemistry (2005): 16931
Authors: van den Berg van Saparoea HB, Lubelski J, van Merkerk R, Mazurkiewicz PS, Driessen AJ.
Journal: Biochemistry (2005): 16931
Role of topoisomerases in cytotoxicity induced by DNA ligand Hoechst-33342 and UV-C in a glioma cell line
Authors: Singh S, Dwarakanath BS, Lazar Mathew T.
Journal: Indian J Exp Biol (2005): 313
Authors: Singh S, Dwarakanath BS, Lazar Mathew T.
Journal: Indian J Exp Biol (2005): 313