Hoechst 34580 *CAS 911004-45-0*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 560.95 |
| Solvent | Water |
Spectral properties
| Excitation (nm) | 371 |
| Emission (nm) | 438 |
Storage, safety and handling
| H-phrase | H303, H313, H340 |
| Hazard symbol | T |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R68 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 41116134 |
Alternative formats
| Hoechst 34580 *20 mM solution in water* |
| Overview |  SDSProtocol |
See also: Nucleus, DNA Methylation, Hoechst DNA Stains for Live and Fixed Cells, Immunohistochemistry (IHC)
CAS 911004-45-0 | Molecular weight 560.95 | Excitation (nm) 371 | Emission (nm) 438 |
The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Hoechst 34580 can be excited by violet laser at 405 nm, and emit blue/cyan fluorescence light around an emission maximum at ~440 nm. It is cell-permeable and provide an excellent tool for flow cytometers equiped with a 405 nm violet laser source.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Hoechst 34580 *CAS 911004-45-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 178.269 µL | 891.345 µL | 1.783 mL | 8.913 mL | 17.827 mL |
| 5 mM | 35.654 µL | 178.269 µL | 356.538 µL | 1.783 mL | 3.565 mL |
| 10 mM | 17.827 µL | 89.135 µL | 178.269 µL | 891.345 µL | 1.783 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| Hoechst 33258 *CAS 23491-45-4* | 352 | 454 | 460001 | 0.03401 |
| Hoechst 33258 *20 mM solution in water* | 352 | 454 | 460001 | 0.03401 |
| Hoechst 33342 *Ultrapure Grade* | 352 | 454 | - | - |
| Hoechst 33342 *20 mM solution in water* | 352 | 454 | - | - |
Images
Figure 1. Chemical structure for Hoechst 34580 *CAS 911004-45-0*
Citations
View all 5 citations: Citation Explorer
A circadian clock translational control mechanism targets specific mRNAs to cytoplasmic messenger ribonucleoprotein granules
Authors: Castillo, Kathrina D and Wu, Cheng and Ding, Zhaolan and Lopez-Garcia, Osiris K and Rowlinson, Emma and Sachs, Matthew S and Bell-Pedersen, Deborah
Journal: Cell Reports (2022): 111879
Authors: Castillo, Kathrina D and Wu, Cheng and Ding, Zhaolan and Lopez-Garcia, Osiris K and Rowlinson, Emma and Sachs, Matthew S and Bell-Pedersen, Deborah
Journal: Cell Reports (2022): 111879
MDA5 Induces a Stronger Interferon Response than RIG-I to GCRV Infection through a Mechanism Involving the Phosphorylation and Dimerization of IRF3 and IRF7 in CIK Cells
Authors: Wan, Quanyuan and Yang, Chunrong and Rao, Youliang and Liao, Zhiwei and Su, Jianguo
Journal: Frontiers in Immunology (2017)
Authors: Wan, Quanyuan and Yang, Chunrong and Rao, Youliang and Liao, Zhiwei and Su, Jianguo
Journal: Frontiers in Immunology (2017)
Grafting of Ring-Opened Cyclopropylamine thin films on Silicon (100) Hydride via UV Photoionization
Authors: Tung, Joline and Ching, Jing Yuan and Ng, Yoke Mooi and Tew, Lih Shin and Khung, Yit Lung
Journal: ACS Applied Materials & Interfaces (2017)
Authors: Tung, Joline and Ching, Jing Yuan and Ng, Yoke Mooi and Tew, Lih Shin and Khung, Yit Lung
Journal: ACS Applied Materials & Interfaces (2017)
Dual-functionalized graphene oxide for enhanced siRNA delivery to breast cancer cells
Authors: Imani, Rana and Shao, Wei and Taherkhani, Samira and Emami, Shahriar Hojjati and Prakash, Satya and Faghihi, Shahab
Journal: Colloids and Surfaces B: Biointerfaces (2016): 315--325
Authors: Imani, Rana and Shao, Wei and Taherkhani, Samira and Emami, Shahriar Hojjati and Prakash, Satya and Faghihi, Shahab
Journal: Colloids and Surfaces B: Biointerfaces (2016): 315--325
A C-terminal acidic domain regulates degradation of the transcriptional coactivator Bob1
Authors: Lindner, John M and Wong, Christina SF and Möller, Andreas and Nielsen, Peter J
Journal: Molecular and cellular biology (2013): 4628--4640
Authors: Lindner, John M and Wong, Christina SF and Möller, Andreas and Nielsen, Peter J
Journal: Molecular and cellular biology (2013): 4628--4640
References
View all 42 references: Citation Explorer
Usefulness of a triple fluorochrome combination Merocyanine 540/Yo-Pro 1/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls
Authors: Hallap T, Nagy S, Jaakma U, Johannisson A, Rodriguez-Martinez H.
Journal: Theriogenology (2006): 1122
Authors: Hallap T, Nagy S, Jaakma U, Johannisson A, Rodriguez-Martinez H.
Journal: Theriogenology (2006): 1122
Fatty acid synthase and its mRNA concentrations are decreased at different times following Hoechst 33342-induced apoptosis in BC3H-1 myocytes
Authors: Zhang X, Kiechle FL.
Journal: Ann Clin Lab Sci (2006): 185
Authors: Zhang X, Kiechle FL.
Journal: Ann Clin Lab Sci (2006): 185
The DNA minor groove binding agents Hoechst 33258 and 33342 enhance recombinant adeno-associated virus (rAAV) transgene expression
Authors: Li L, Yang L, Kotin RM.
Journal: J Gene Med (2005): 420
Authors: Li L, Yang L, Kotin RM.
Journal: J Gene Med (2005): 420
Resistance mechanism development to the topoisomerase-I inhibitor Hoechst 33342 by Leishmania donovani
Authors: Marquis JF, Hardy I, Olivier M.
Journal: Parasitology (2005): 197
Authors: Marquis JF, Hardy I, Olivier M.
Journal: Parasitology (2005): 197
Acid-base and electronic structure-dependent properties of Hoechst 33342
Authors: Aleman C, Namba AM, Casanovas J.
Journal: J Biomol Struct Dyn (2005): 29
Authors: Aleman C, Namba AM, Casanovas J.
Journal: J Biomol Struct Dyn (2005): 29
Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry
Authors: Cai K, Yang J, Guan M, Ji W, Li Y, Rens W.
Journal: Arch Androl (2005): 371
Authors: Cai K, Yang J, Guan M, Ji W, Li Y, Rens W.
Journal: Arch Androl (2005): 371
Evidence for a qualitative hierarchy within the Hoechst-33342 'side population' (SP) of murine bone marrow cells
Authors: Robinson SN, Seina SM, Gohr JC, Kuszynski CA, Sharp JG.
Journal: Bone Marrow Transplant (2005): 807
Authors: Robinson SN, Seina SM, Gohr JC, Kuszynski CA, Sharp JG.
Journal: Bone Marrow Transplant (2005): 807
Flow cytometric characterization of viable meiotic and postmeiotic cells by Hoechst 33342 in mouse spermatogenesis
Authors: Bastos H, Lassalle B, Chicheportiche A, Riou L, Testart J, Allem and I, Fouchet P.
Journal: Cytometry A (2005): 40
Authors: Bastos H, Lassalle B, Chicheportiche A, Riou L, Testart J, Allem and I, Fouchet P.
Journal: Cytometry A (2005): 40
An in vitro study of Hoechst 33342 redistribution and its effects on cell viability
Authors: Mohorko N, Kregar-Velikonja N, Repovs G, Gorensek M, Bresjanac M.
Journal: Hum Exp Toxicol (2005): 573
Authors: Mohorko N, Kregar-Velikonja N, Repovs G, Gorensek M, Bresjanac M.
Journal: Hum Exp Toxicol (2005): 573
Role of topoisomerases in cytotoxicity induced by DNA ligand Hoechst-33342 and UV-C in a glioma cell line
Authors: Singh S, Dwarakanath BS, Lazar Mathew T.
Journal: Indian J Exp Biol (2005): 313
Authors: Singh S, Dwarakanath BS, Lazar Mathew T.
Journal: Indian J Exp Biol (2005): 313