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Hoechst 34580 *CAS 911004-45-0*

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Physical properties
Molecular weight560.95
Spectral properties
Excitation (nm)371
Emission (nm)438
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Hoechst 34580 can be excited by violet laser at 405 nm, and emit blue/cyan fluorescence light around an emission maximum at ~440 nm. It is cell-permeable and provide an excellent tool for flow cytometers equiped with a 405 nm violet laser source.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Hoechst 34580 *CAS 911004-45-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM178.269 µL891.345 µL1.783 mL8.913 mL17.827 mL
5 mM35.654 µL178.269 µL356.538 µL1.783 mL3.565 mL
10 mM17.827 µL89.135 µL178.269 µL891.345 µL1.783 mL

Molarity calculator

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Spectral properties

Excitation (nm)371
Emission (nm)438

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yield
Hoechst 33258 *CAS 23491-45-4*3524544600010.03401
Hoechst 33258 *20 mM solution in water*3524544600010.03401
Hoechst 33342 *Ultrapure Grade*352454--
Hoechst 33342 *20 mM solution in water*352454--



View all 5 citations: Citation Explorer
A circadian clock translational control mechanism targets specific mRNAs to cytoplasmic messenger ribonucleoprotein granules
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Journal: Cell Reports (2022): 111879
MDA5 Induces a Stronger Interferon Response than RIG-I to GCRV Infection through a Mechanism Involving the Phosphorylation and Dimerization of IRF3 and IRF7 in CIK Cells
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Journal: Frontiers in Immunology (2017)
Grafting of Ring-Opened Cyclopropylamine thin films on Silicon (100) Hydride via UV Photoionization
Authors: Tung, Joline and Ching, Jing Yuan and Ng, Yoke Mooi and Tew, Lih Shin and Khung, Yit Lung
Journal: ACS Applied Materials &amp; Interfaces (2017)
Dual-functionalized graphene oxide for enhanced siRNA delivery to breast cancer cells
Authors: Imani, Rana and Shao, Wei and Taherkhani, Samira and Emami, Shahriar Hojjati and Prakash, Satya and Faghihi, Shahab
Journal: Colloids and Surfaces B: Biointerfaces (2016): 315--325
A C-terminal acidic domain regulates degradation of the transcriptional coactivator Bob1
Authors: Lindner, John M and Wong, Christina SF and M&ouml;ller, Andreas and Nielsen, Peter J
Journal: Molecular and cellular biology (2013): 4628--4640


View all 42 references: Citation Explorer
Usefulness of a triple fluorochrome combination Merocyanine 540/Yo-Pro 1/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls
Authors: Hallap T, Nagy S, Jaakma U, Johannisson A, Rodriguez-Martinez H.
Journal: Theriogenology (2006): 1122
Fatty acid synthase and its mRNA concentrations are decreased at different times following Hoechst 33342-induced apoptosis in BC3H-1 myocytes
Authors: Zhang X, Kiechle FL.
Journal: Ann Clin Lab Sci (2006): 185
Evidence for a qualitative hierarchy within the Hoechst-33342 'side population' (SP) of murine bone marrow cells
Authors: Robinson SN, Seina SM, Gohr JC, Kuszynski CA, Sharp JG.
Journal: Bone Marrow Transplant (2005): 807
Proton motive force-dependent Hoechst 33342 transport by the ABC transporter LmrA of Lactococcus lactis
Authors: van den Berg van Saparoea HB, Lubelski J, van Merkerk R, Mazurkiewicz PS, Driessen AJ.
Journal: Biochemistry (2005): 16931
Role of topoisomerases in cytotoxicity induced by DNA ligand Hoechst-33342 and UV-C in a glioma cell line
Authors: Singh S, Dwarakanath BS, Lazar Mathew T.
Journal: Indian J Exp Biol (2005): 313
An in vitro study of Hoechst 33342 redistribution and its effects on cell viability
Authors: Mohorko N, Kregar-Velikonja N, Repovs G, Gorensek M, Bresjanac M.
Journal: Hum Exp Toxicol (2005): 573
Flow cytometric characterization of viable meiotic and postmeiotic cells by Hoechst 33342 in mouse spermatogenesis
Authors: Bastos H, Lassalle B, Chicheportiche A, Riou L, Testart J, Allem and I, Fouchet P.
Journal: Cytometry A (2005): 40
Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry
Authors: Cai K, Yang J, Guan M, Ji W, Li Y, Rens W.
Journal: Arch Androl (2005): 371
Acid-base and electronic structure-dependent properties of Hoechst 33342
Authors: Aleman C, Namba AM, Casanovas J.
Journal: J Biomol Struct Dyn (2005): 29
Resistance mechanism development to the topoisomerase-I inhibitor Hoechst 33342 by Leishmania donovani
Authors: Marquis JF, Hardy I, Olivier M.
Journal: Parasitology (2005): 197