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Horseradish Peroxidase *Optimized for conjugation*

Horseradish peroxidase (HRP) is a glycoprotein with a molecular weight of ~44 KD. It is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine. HRP readily combines with hydrogen peroxide (H2O2), and the resultant [HRP–H2O2] complex can oxidize a wide variety of chromogenic hydrogen donors. HRP is widely used as a label for immunoglobulins in many different immunochemistry applications, including ELISA, immunoblotting and immunohistochemistry. AAT Bioquest offers the largest collection of HRP substrates and conjugates.
Horseradish peroxidase (HRP) is a glycoprotein with a molecular weight of ~44 KD. It is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine. HRP readily combines with hydrogen peroxide (H2O2), and the resultant [HRP–H2O2] complex can oxidize a wide variety of chromogenic hydrogen donors. HRP is widely used as a label for immunoglobulins in many different immunochemistry applications, including ELISA, immunoblotting and immunohistochemistry. AAT Bioquest offers the largest collection of HRP substrates and conjugates.
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Price ()
Catalog Number11021
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventWater
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Horseradish peroxidase (HRP) is a glycoprotein with a molecular weight of ~44 KD. It is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine. HRP readily combines with hydrogen peroxide (H2O2), and the resultant [HRP–H2O2] complex can oxidize a wide variety of chromogenic hydrogen donors. AAT Bioquest offers the largest collection of HRP substrates and conjugates. HRP is widely used as a label for immunoglobulins in many different immunochemistry applications, including ELISA, immunoblotting and immunohistochemistry. HRP can be conjugated to antibodies by several different methods, including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-inkers. Our Buccutite™ HRP conjugation technique is by far the most effective method. HRP is the most desired label for antibodies since it is the smallest and most stable of the three most popular enzyme labels (HRP, alkaline phosphatase, and B-galactosidase), and its glycosylation leads to lower non-specific binding. This purified horseradish peroxidase (HRP) is supplied lyophilized as a salt-free powder for reconstitution and use in HRP detections or conjugations.

References


View all 50 references: Citation Explorer
Nanocapsulation of horseradish peroxidase (HRP) enhances enzymatic performance in removing phenolic compounds.
Authors: Liu, Shan and Huang, Biyan and Zheng, Guiqin and Zhang, Peng and Li, Juying and Yang, Bo and Chen, Yantao and Liang, Li
Journal: International journal of biological macromolecules (2020): 814-822
Antibody-biotin-streptavidin-horseradish peroxidase (HRP) sensor for rapid and ultra-sensitive detection of fumonisins.
Authors: Yang, Hualin and Zhang, Qi and Liu, Xiaolei and Yang, Yuying and Yang, Yong and Liu, Mingyuan and Li, Peiwu and Zhou, Yu
Journal: Food chemistry (2020): 126356
An efficient methodology for the purification of date palm peroxidase: Stability comparison with horseradish peroxidase (HRP).
Authors: Saud Al-Bagmi, Moneera and Shahnawaz Khan, Mohd and Alhasan Ismael, Mohamad and Al-Senaidy, Abdulrahman M and Ben Bacha, Abir and Mabood Husain, Fohad and Alamery, Salman Freeh
Journal: Saudi journal of biological sciences (2019): 301-307
Sensitive sulfide ion detection by optofluidic catalytic laser using horseradish peroxidase (HRP) enzyme.
Authors: Gong, Chaoyang and Gong, Yuan and Khaing Oo, Maung Kyaw and Wu, Yu and Rao, Yunjiang and Tan, Xiaotian and Fan, Xudong
Journal: Biosensors & bioelectronics (2017): 351-357
Ultrasensitive electrochemical immunosensor based on horseradish peroxidase (HRP)-loaded silica-poly(acrylic acid) brushes for protein biomarker detection.
Authors: Zhao, Yan and Zheng, Yiqun and Kong, Rongmei and Xia, Lian and Qu, Fengli
Journal: Biosensors & bioelectronics (2016): 383-8
Bioconjugation of Antibodies to Horseradish Peroxidase (HRP).
Authors: Hnasko, Robert M
Journal: Methods in molecular biology (Clifton, N.J.) (2015): 43-50
Pb(2+)-introduced activation of horseradish peroxidase (HRP)-mimicking DNAzyme.
Authors: Zhu, Xi and Gao, Xiaoyao and Liu, Qida and Lin, Zhenyu and Qiu, Bin and Chen, Guonan
Journal: Chemical communications (Cambridge, England) (2011): 7437-9
Toxicity of textile dyes and their degradation by the enzyme horseradish peroxidase (HRP).
Authors: Ulson de Souza, Selene Maria Arruda Guelli and Forgiarini, Eliane and Ulson de Souza, Antônio Augusto
Journal: Journal of hazardous materials (2007): 1073-8
Studies on the oxidation reaction of tyrosine (Tyr) with H2O2 catalyzed by horseradish peroxidase (HRP) in alcohol-water medium by spectrofluorimetry and differential spectrophotometry.
Authors: Tang, Bo and Wang, Yan and Liang, Huiling and Chen, Zhenzhen and He, Xiwen and Shen, Hanxi
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2006): 609-13
FIA-near-infrared spectrofluorimetric trace determination of hydrogen peroxide using tricarchlorobocyanine dye (Cy.7.Cl) and horseradish peroxidase (HRP).
Authors: Tang, Bo and Zhang, Li and Xu, Ke-hua
Journal: Talanta (2006): 876-82