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hOVA *Optimized for Maximum Immunization Response*

Rabbits are immunized with BSA, BSA-AHD (Aminohydantoin), cBSA-AHD and hBSA-AHD conjugates. Antisera from immunized rabbits are tested for the immune response against AHD.   Data indicate  cBSA-AHD and hBSA-AHD exhibit a stronger immune response to AHD in comparison with BSA-AHD. Similar results are obtained for cOval and hOval conjugates (Mol Pharm. 2011 August 1; 8(4): 1174–1185).
Rabbits are immunized with BSA, BSA-AHD (Aminohydantoin), cBSA-AHD and hBSA-AHD conjugates. Antisera from immunized rabbits are tested for the immune response against AHD.   Data indicate  cBSA-AHD and hBSA-AHD exhibit a stronger immune response to AHD in comparison with BSA-AHD. Similar results are obtained for cOval and hOval conjugates (Mol Pharm. 2011 August 1; 8(4): 1174–1185).
Rabbits are immunized with BSA, BSA-AHD (Aminohydantoin), cBSA-AHD and hBSA-AHD conjugates. Antisera from immunized rabbits are tested for the immune response against AHD.   Data indicate  cBSA-AHD and hBSA-AHD exhibit a stronger immune response to AHD in comparison with BSA-AHD. Similar results are obtained for cOval and hOval conjugates (Mol Pharm. 2011 August 1; 8(4): 1174–1185).
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Physical properties
Molecular weight60000
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C)
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
60000
hOVA is the ovalbumin (OVA) modified with hexamethylenediamine to make the proteins bear more amine groups (thus more positive charges at neutral pH). It is proven to be a superior carrier protein for developing antibodies since hOVA conjugates have stronger imunological responses than the unmodifed OVA cinjugates.

Calculators


Common stock solution preparation

Table 1. Volume of appropriate solvent needed to reconstitute specific mass of hOVA *Optimized for Maximum Immunization Response* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM1.667 µL8.333 µL16.667 µL83.333 µL166.667 µL
5 mM333.333 nL1.667 µL3.333 µL16.667 µL33.333 µL
10 mM166.667 nL833.333 nL1.667 µL8.333 µL16.667 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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References


View all 29 references: Citation Explorer
Optimization of the preparation process of vinblastine sulfate (VBLS)-loaded folate-conjugated bovine serum albumin (BSA) nanoparticles for tumor-targeted drug delivery using response surface methodology (RSM)
Authors: Zu Y, Zhang Y, Zhao X, Zhang Q, Liu Y, Jiang R.
Journal: Int J Nanomedicine (2009): 321
Characterization of bovine serum albumin glycated with glucose, galactose and lactose
Authors: Ledesma-Osuna AI, Ramos-Clamont G, Vazquez-Moreno L.
Journal: Acta Biochim Pol (2008): 491
Improvement of functional properties of bovine serum albumin through phosphorylation by dry-heating in the presence of pyrophosphate
Authors: Enomoto H, Li CP, Morizane K, Ibrahim HR, Sugimoto Y, Ohki S, Ohtomo H, Aoki T.
Journal: J Food Sci (2008): C84
Polyethylene glycol improves conjugation of bovine hemoglobin and human serum albumin in a controlled ratio
Authors: Zheng C, Bi J, Ma G, Su Z.
Journal: Artif Cells Blood Substit Immobil Biotechnol (2007): 568
Purification of denatured bovine serum albumin coated CdTe quantum dots for sensitive detection of silver(I) ions
Authors: Wang JH, Wang HQ, Zhang HL, Li XQ, Hua XF, Cao YC, Huang ZL, Zhao YD.
Journal: Anal Bioanal Chem (2007): 969
Effect of lyophilization on the structure and phase changes of PEGylated-bovine serum albumin
Authors: Tattini V, Jr., Parra DF, Polakiewicz B, Pitombo RN.
Journal: Int J Pharm (2005): 124
Preparation of bovine serum albumin nanoparticles surface-modified with glycyrrhizin
Authors: Mao SJ, Hou SX, Zhang LK, Jin H, Bi YQ, Jiang B.
Journal: Yao Xue Xue Bao (2003): 787
Products of spontaneous conjugation of aflatoxins with bovine serum albumin: immunochemical properties
Authors: Burkin AA, Kononenko GP, Soboleva NA.
Journal: Prikl Biokhim Mikrobiol (2003): 228
Generation of polyclonal antibody against mu-conotoxin GIIIA using an immunogen of [Cys(5)]mu-conotoxin GIIIA site-specifically conjugated with bovine serum albumin
Authors: Nakamura M, Oba Y, Mori T, Sato K, Ishida Y, Matsuda T, Nakamura H.
Journal: Biochem Biophys Res Commun (2002): 1037
Mice vaccinated with the O-antigen of Francisella tularensis LVS lipopolysaccharide conjugated to bovine serum albumin develop varying degrees of protective immunity against systemic or aerosol challenge with virulent type A and type B strains of the patho
Authors: Conlan JW, Shen H, Webb A, Perry MB.
Journal: Vaccine (2002): 3465